LOXL null mice demonstrate selective dentate structural changes but maintain dentate granule cell and CA1 pyramidal cell potentiation in the hippocampus

Nathan W. Bronson, Jock S. Hamilton, Mei Han, Ping An Li, Ian Hornstra, John M. Horowitz, Barbara A Horwitz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Lysyl oxidase-like protein (LOXL), part of the lysyl oxidase copper-dependent amine oxidase family, is expressed in the extracellular matrix and in the nucleus. It likely plays a role in cross-linking collagen and elastin, possibly modulating cellular functions. Immunohistochemical studies show the presence of LOXL in the pyramidal cell layer of the hippocampus; and in this study, we report that cells in the granule cell layer have significantly smaller somas in LOXL -/- compared to LOXL +/+ mice. In addition we tested the hypothesis that these structural alterations in the dentate granule layer were associated with synaptic efficacy and thus muted long-term potentiation in mice lacking the protein. Electrical recordings were obtained in 300-μm hippocampal slices in dentate and CA1 pyramidal cell layers in age-matched wild type and LOXL null mice. Potentiation in the CA1 cell layer of 10 LOXL -/- and 8 LOXL +/+ mice was 191.0 ± 9.3% and 181.6 ± 9.1%, respectively (mean ± S.E.M.). Dentate potentiation was 120.8 ± 7.0% and 121.0 ± 3.4% in 11 LOXL -/- and 11 LOXL +/+ mice, respectively. No phenotypic difference in potentiation of population spike amplitude (or in EPSP slope) in either layer was observed. Thus, contrary to expectation, structural changes in the hippocampus of LOXL -/- mice did not affect synaptic remodeling in a manner that impaired the establishment of LTP.

Original languageEnglish (US)
Pages (from-to)118-122
Number of pages5
JournalNeuroscience Letters
Volume390
Issue number2
DOIs
StatePublished - Dec 23 2005

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Protein-Lysine 6-Oxidase
Pyramidal Cells
Hippocampus
Proteins
Amine Oxidase (Copper-Containing)
Hippocampal CA1 Region
Elastin
Long-Term Potentiation
Excitatory Postsynaptic Potentials
Carisoprodol
Extracellular Matrix

Keywords

  • Dentate granular layer
  • Hippocampus
  • Long term potentiation
  • Lysyl oxidase-like protein
  • Mice
  • Morphology

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

LOXL null mice demonstrate selective dentate structural changes but maintain dentate granule cell and CA1 pyramidal cell potentiation in the hippocampus. / Bronson, Nathan W.; Hamilton, Jock S.; Han, Mei; Li, Ping An; Hornstra, Ian; Horowitz, John M.; Horwitz, Barbara A.

In: Neuroscience Letters, Vol. 390, No. 2, 23.12.2005, p. 118-122.

Research output: Contribution to journalArticle

Bronson, Nathan W. ; Hamilton, Jock S. ; Han, Mei ; Li, Ping An ; Hornstra, Ian ; Horowitz, John M. ; Horwitz, Barbara A. / LOXL null mice demonstrate selective dentate structural changes but maintain dentate granule cell and CA1 pyramidal cell potentiation in the hippocampus. In: Neuroscience Letters. 2005 ; Vol. 390, No. 2. pp. 118-122.
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AU - Li, Ping An

AU - Hornstra, Ian

AU - Horowitz, John M.

AU - Horwitz, Barbara A

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