LOX-1 mediates lysophosphatidylcholine-induced oxidized LDL uptake in smooth muscle cells

Takuma Aoyama, Mingyi Chen, Hisayoshi Fujiwara, Tomoh Masaki, Tatsuya Sawamura

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

A novel receptor for oxidized low-density lipoprotein (OxLDL), lectin-like OxLDL receptor (LOX-1), was cloned from endothelial cells. Since OxLDL is taken up by vascular smooth muscle cells (VSMC) in atheroma, we analyzed the inducible expression of LOX-1 in VSMC in the present study. Incubation of cultured bovine VSMC with lysophosphatidylcholine (LPC), an atherogenic component of OxLDL, increased the level of mRNA for LOX-1 in a dose- and time-dependent manner. Since LPC did not significantly change the half-life of LOX-1 mRNA, the induction seemed to occur at the transcriptional level. The induction accompanied an increase in the protein level of LOX-1 and activity of OxLDL uptake. Blocking antibody against LOX-1 significantly suppressed the enhanced uptake of OxLDL. Thus, LOX-1 is a major receptor for OxLDL in VSMC as in endothelial cells. The enhanced expression of LOX-1 by LPC suggests that OxLDL and LPC would progressively change the function of VSMC and accelerate atherogenesis in vivo. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)217-220
Number of pages4
JournalFEBS Letters
Volume467
Issue number2-3
DOIs
StatePublished - Feb 11 2000
Externally publishedYes

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Keywords

  • Atherosclerosis
  • Lectin-like oxidized low-density lipoprotein receptor
  • Lysophosphatidylcholine
  • Oxidized LDL
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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