Low-Z polymer sample supports for fixed-target serial femtosecond X-ray crystallography

Geoffrey K. Feld, Michael Heymann, W. Henry Benner, Tommaso Pardini, Ching Ju Tsai, Sébastien Boutet, Matthew A Coleman, Mark S. Hunter, Xiaodan Li, Marc Messerschmidt, Achini Opathalage, Bill Pedrini, Garth J. Williams, Bryan A. Krantz, Seth Fraden, Stefan Hau-Riege, James E. Evans, Brent W. Segelke, Matthias Frank

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

X-ray free-electron lasers (XFELs) offer a new avenue to the structural probing of complex materials, including biomolecules. Delivery of precious sample to the XFEL beam is a key consideration, as the sample of interest must be serially replaced after each destructive pulse. The fixed-target approach to sample delivery involves depositing samples on a thin-film support and subsequent serial introduction via a translating stage. Some classes of biological materials, including two-dimensional protein crystals, must be introduced on fixed-target supports, as they require a flat surface to prevent sample wrinkling. A series of wafer and transmission electron microscopy (TEM)-style grid supports constructed of low-Z plastic have been custom-designed and produced. Aluminium TEM grid holders were engineered, capable of delivering up to 20 different conventional or plastic TEM grids using fixed-target stages available at the Linac Coherent Light Source (LCLS). As proof-of-principle, X-ray diffraction has been demonstrated from two-dimensional crystals of bacteriorhodopsin and three-dimensional crystals of anthrax toxin protective antigen mounted on these supports at the LCLS. The benefits and limitations of these low-Z fixed-target supports are discussed; it is the authors' belief that they represent a viable and efficient alternative to previously reported fixed-target supports for conducting diffraction studies with XFELs.

Original languageEnglish (US)
Pages (from-to)1072-1079
Number of pages8
JournalJournal of Applied Crystallography
Volume48
DOIs
StatePublished - Aug 1 2015
Externally publishedYes

Fingerprint

X ray lasers
X ray crystallography
Free electron lasers
X Ray Crystallography
Transmission Electron Microscopy
Polymers
Lasers
X-Rays
Electrons
Transmission electron microscopy
Crystals
Plastics
Light sources
Bacteriorhodopsins
Light
Biomolecules
Aluminum
X-Ray Diffraction
Biological materials
Laser beams

Keywords

  • biological crystallography
  • microcrystallography
  • serial femtosecond crystallography
  • two-dimensional crystallography
  • X-ray free-electron lasers

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Low-Z polymer sample supports for fixed-target serial femtosecond X-ray crystallography. / Feld, Geoffrey K.; Heymann, Michael; Benner, W. Henry; Pardini, Tommaso; Tsai, Ching Ju; Boutet, Sébastien; Coleman, Matthew A; Hunter, Mark S.; Li, Xiaodan; Messerschmidt, Marc; Opathalage, Achini; Pedrini, Bill; Williams, Garth J.; Krantz, Bryan A.; Fraden, Seth; Hau-Riege, Stefan; Evans, James E.; Segelke, Brent W.; Frank, Matthias.

In: Journal of Applied Crystallography, Vol. 48, 01.08.2015, p. 1072-1079.

Research output: Contribution to journalArticle

Feld, GK, Heymann, M, Benner, WH, Pardini, T, Tsai, CJ, Boutet, S, Coleman, MA, Hunter, MS, Li, X, Messerschmidt, M, Opathalage, A, Pedrini, B, Williams, GJ, Krantz, BA, Fraden, S, Hau-Riege, S, Evans, JE, Segelke, BW & Frank, M 2015, 'Low-Z polymer sample supports for fixed-target serial femtosecond X-ray crystallography', Journal of Applied Crystallography, vol. 48, pp. 1072-1079. https://doi.org/10.1107/S1600576715010493
Feld, Geoffrey K. ; Heymann, Michael ; Benner, W. Henry ; Pardini, Tommaso ; Tsai, Ching Ju ; Boutet, Sébastien ; Coleman, Matthew A ; Hunter, Mark S. ; Li, Xiaodan ; Messerschmidt, Marc ; Opathalage, Achini ; Pedrini, Bill ; Williams, Garth J. ; Krantz, Bryan A. ; Fraden, Seth ; Hau-Riege, Stefan ; Evans, James E. ; Segelke, Brent W. ; Frank, Matthias. / Low-Z polymer sample supports for fixed-target serial femtosecond X-ray crystallography. In: Journal of Applied Crystallography. 2015 ; Vol. 48. pp. 1072-1079.
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abstract = "X-ray free-electron lasers (XFELs) offer a new avenue to the structural probing of complex materials, including biomolecules. Delivery of precious sample to the XFEL beam is a key consideration, as the sample of interest must be serially replaced after each destructive pulse. The fixed-target approach to sample delivery involves depositing samples on a thin-film support and subsequent serial introduction via a translating stage. Some classes of biological materials, including two-dimensional protein crystals, must be introduced on fixed-target supports, as they require a flat surface to prevent sample wrinkling. A series of wafer and transmission electron microscopy (TEM)-style grid supports constructed of low-Z plastic have been custom-designed and produced. Aluminium TEM grid holders were engineered, capable of delivering up to 20 different conventional or plastic TEM grids using fixed-target stages available at the Linac Coherent Light Source (LCLS). As proof-of-principle, X-ray diffraction has been demonstrated from two-dimensional crystals of bacteriorhodopsin and three-dimensional crystals of anthrax toxin protective antigen mounted on these supports at the LCLS. The benefits and limitations of these low-Z fixed-target supports are discussed; it is the authors' belief that they represent a viable and efficient alternative to previously reported fixed-target supports for conducting diffraction studies with XFELs.",
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AU - Feld, Geoffrey K.

AU - Heymann, Michael

AU - Benner, W. Henry

AU - Pardini, Tommaso

AU - Tsai, Ching Ju

AU - Boutet, Sébastien

AU - Coleman, Matthew A

AU - Hunter, Mark S.

AU - Li, Xiaodan

AU - Messerschmidt, Marc

AU - Opathalage, Achini

AU - Pedrini, Bill

AU - Williams, Garth J.

AU - Krantz, Bryan A.

AU - Fraden, Seth

AU - Hau-Riege, Stefan

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