Low plasma leptin levels contribute to diabetic hyperphagia in rats

Dana K. Sindelar, Peter J Havel, Randy J. Seeley, Charles W. Wilkinson, Stephen C. Woods, Michael W. Schwartz

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The adipocyte hormone leptin reduces food intake in normal animals. During uncontrolled type 1 diabetes, plasma leptin levels fall, whereas food intake increases. To test the hypothesis that low leptin levels contribute to diabetic hyperphagia, we investigated the effect on food intake of replacement of leptin at basal plasma concentrations for 7 days in Long- Evans rats with uncontrolled diabetes induced by streptozotocin (STZ). One group of STZ diabetic rats received saline (STZ + Sal) (n = 11), while the other group (STZ + Lep) (n = 15) received a subcutaneous infusion of recombinant rat leptin (100 μg · kg-1 · day-1) via osmotic minipumps. A nondiabetic control group (Con) (n = 11) received saline only. In the STZ + Sal group, plasma leptin levels decreased by 75% (P < 0.05) from 2.4 ± 0.5 on the day before STZ/citrate buffer vehicle (Veh) injection (day 0) to 0.6 ± 0.2 ng/ml on day 7. In contrast, plasma leptin levels on days 3-7 were comparable to pretreatment values in both the STZ + Lep group (day 0: 2.6 ± 0.4 vs. day 7: 2.5 ± 0.3 ng/ml, NS) and the Con group (day 0: 3.8 ± 0.4 vs. day 7: 2.9 ± 1.0 ng/ml, NS). In the STZ + Sal group, daily food intake increased gradually to values 43% above basal by day 7 (day 0: 24 ± 2 to day 7: 33 ± 3 g, P < 0.05), whereas food intake did not increase in either the STZ + Lep group (day 0: 24 ± 1 vs. day 7: 21 ± 2 g, NS), or the Con group (day 0: 23 ± 1 vs. day 7: 23 ± 2 g). Plasma glucose levels exceeded nondiabetic control values (7.7 ± 0.2 mmol/l) in both diabetic groups, but were lower in the STZ + Lep group (17.2 ± 1.8 mmol/l) than in the STZ + Sal group (24.3 ± 1.1 mmol/l, P < 0.05). To determine if sensitivity to leptin- induced anorexia was affected by STZ treatment, a second experiment was performed in which the effect of intracerebroventricular leptin injection (at doses of 0.35, 1.0, or 3.5 μg) on food intake was measured 10 days after STZ or Veh treatment. Leptin suppressed both 4- and 24-h food intake in the two groups to an equal extent at every dose (by 152 22, and 35%, respectively). These findings support the hypothesis that the effect of uncontrolled diabetes to lower leptin levels contributes to diabetic hyperphagia and that this effect is not due to altered leptin sensitivity.

Original languageEnglish (US)
Pages (from-to)1275-1280
Number of pages6
JournalDiabetes
Volume48
Issue number6
DOIs
StatePublished - Jun 2000

Fingerprint

Hyperphagia
Streptozocin
Leptin
Eating
Subcutaneous Infusions
Long Evans Rats
Injections
Experimental Diabetes Mellitus
Anorexia
Type 1 Diabetes Mellitus
Adipocytes
Citric Acid
Buffers

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Sindelar, D. K., Havel, P. J., Seeley, R. J., Wilkinson, C. W., Woods, S. C., & Schwartz, M. W. (2000). Low plasma leptin levels contribute to diabetic hyperphagia in rats. Diabetes, 48(6), 1275-1280. https://doi.org/10.2337/diabetes.48.6.1275

Low plasma leptin levels contribute to diabetic hyperphagia in rats. / Sindelar, Dana K.; Havel, Peter J; Seeley, Randy J.; Wilkinson, Charles W.; Woods, Stephen C.; Schwartz, Michael W.

In: Diabetes, Vol. 48, No. 6, 06.2000, p. 1275-1280.

Research output: Contribution to journalArticle

Sindelar, DK, Havel, PJ, Seeley, RJ, Wilkinson, CW, Woods, SC & Schwartz, MW 2000, 'Low plasma leptin levels contribute to diabetic hyperphagia in rats', Diabetes, vol. 48, no. 6, pp. 1275-1280. https://doi.org/10.2337/diabetes.48.6.1275
Sindelar DK, Havel PJ, Seeley RJ, Wilkinson CW, Woods SC, Schwartz MW. Low plasma leptin levels contribute to diabetic hyperphagia in rats. Diabetes. 2000 Jun;48(6):1275-1280. https://doi.org/10.2337/diabetes.48.6.1275
Sindelar, Dana K. ; Havel, Peter J ; Seeley, Randy J. ; Wilkinson, Charles W. ; Woods, Stephen C. ; Schwartz, Michael W. / Low plasma leptin levels contribute to diabetic hyperphagia in rats. In: Diabetes. 2000 ; Vol. 48, No. 6. pp. 1275-1280.
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N2 - The adipocyte hormone leptin reduces food intake in normal animals. During uncontrolled type 1 diabetes, plasma leptin levels fall, whereas food intake increases. To test the hypothesis that low leptin levels contribute to diabetic hyperphagia, we investigated the effect on food intake of replacement of leptin at basal plasma concentrations for 7 days in Long- Evans rats with uncontrolled diabetes induced by streptozotocin (STZ). One group of STZ diabetic rats received saline (STZ + Sal) (n = 11), while the other group (STZ + Lep) (n = 15) received a subcutaneous infusion of recombinant rat leptin (100 μg · kg-1 · day-1) via osmotic minipumps. A nondiabetic control group (Con) (n = 11) received saline only. In the STZ + Sal group, plasma leptin levels decreased by 75% (P < 0.05) from 2.4 ± 0.5 on the day before STZ/citrate buffer vehicle (Veh) injection (day 0) to 0.6 ± 0.2 ng/ml on day 7. In contrast, plasma leptin levels on days 3-7 were comparable to pretreatment values in both the STZ + Lep group (day 0: 2.6 ± 0.4 vs. day 7: 2.5 ± 0.3 ng/ml, NS) and the Con group (day 0: 3.8 ± 0.4 vs. day 7: 2.9 ± 1.0 ng/ml, NS). In the STZ + Sal group, daily food intake increased gradually to values 43% above basal by day 7 (day 0: 24 ± 2 to day 7: 33 ± 3 g, P < 0.05), whereas food intake did not increase in either the STZ + Lep group (day 0: 24 ± 1 vs. day 7: 21 ± 2 g, NS), or the Con group (day 0: 23 ± 1 vs. day 7: 23 ± 2 g). Plasma glucose levels exceeded nondiabetic control values (7.7 ± 0.2 mmol/l) in both diabetic groups, but were lower in the STZ + Lep group (17.2 ± 1.8 mmol/l) than in the STZ + Sal group (24.3 ± 1.1 mmol/l, P < 0.05). To determine if sensitivity to leptin- induced anorexia was affected by STZ treatment, a second experiment was performed in which the effect of intracerebroventricular leptin injection (at doses of 0.35, 1.0, or 3.5 μg) on food intake was measured 10 days after STZ or Veh treatment. Leptin suppressed both 4- and 24-h food intake in the two groups to an equal extent at every dose (by 152 22, and 35%, respectively). These findings support the hypothesis that the effect of uncontrolled diabetes to lower leptin levels contributes to diabetic hyperphagia and that this effect is not due to altered leptin sensitivity.

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