Low levels of physiological interstitial flow eliminate morphogen gradients and guide angiogenesis

Venktesh S. Shirure, Andrew Lezia, Arnold Tao, Luis F. Alonzo, Steven George

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Convective transport can significantly distort spatial concentration gradients. Interstitial flow is ubiquitous throughout living tissue, but our understanding of how interstitial flow affects concentration gradients in biological processes is limited. Interstitial flow is of particular interest for angiogenesis because pathological and physiological angiogenesis is associated with altered interstitial flow, and both interstitial flow and morphogen gradients (e.g., vascular endothelial growth factor, VEGF) can potentially stimulate and guide new blood vessel growth. We designed an in vitro microfluidic platform to simulate 3D angiogenesis in a tissue microenvironment that precisely controls interstitial flow and spatial morphogen gradients. The microvascular tissue was developed from endothelial colony forming cell-derived endothelial cells extracted from cord blood and stromal fibroblasts in a fibrin extracellular matrix. Pressure in the microfluidic lines was manipulated to control the interstitial flow. A mathematical model of mass and momentum transport, and experimental studies with fluorescently labeled dextran were performed to validate the platform. Our data demonstrate that at physiological interstitial flow (0.1–10 μm/s), morphogen gradients were eliminated within hours, and angiogenesis demonstrated a striking bias in the opposite direction of interstitial flow. The interstitial flow-directed angiogenesis was dependent on the presence of VEGF, and the effect was mediated by αvβ3 integrin. We conclude that under physiological conditions, growth factors such as VEGF and fluid forces work together to initiate and spatially guide angiogenesis.

Original languageEnglish (US)
Pages (from-to)493-504
Number of pages12
JournalAngiogenesis
Volume20
Issue number4
DOIs
StatePublished - Nov 1 2017
Externally publishedYes

Fingerprint

Vascular Endothelial Growth Factor A
Microfluidics
Tissue
Physiologic Neovascularization
Pathologic Neovascularization
Biological Phenomena
Endothelial cells
Blood vessels
Fibroblasts
Dextrans
Fibrin
Fetal Blood
Integrins
Extracellular Matrix
Blood Vessels
Intercellular Signaling Peptides and Proteins
Momentum
Blood
Theoretical Models
Endothelial Cells

Keywords

  • Concentration gradients
  • Microphysiological systems
  • Organ-on-a-chip
  • αvβ3 integrin

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cancer Research

Cite this

Low levels of physiological interstitial flow eliminate morphogen gradients and guide angiogenesis. / Shirure, Venktesh S.; Lezia, Andrew; Tao, Arnold; Alonzo, Luis F.; George, Steven.

In: Angiogenesis, Vol. 20, No. 4, 01.11.2017, p. 493-504.

Research output: Contribution to journalArticle

Shirure, Venktesh S. ; Lezia, Andrew ; Tao, Arnold ; Alonzo, Luis F. ; George, Steven. / Low levels of physiological interstitial flow eliminate morphogen gradients and guide angiogenesis. In: Angiogenesis. 2017 ; Vol. 20, No. 4. pp. 493-504.
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