Abstract
Background - Although diabetes confers an increased propensity toward accelerated atherogenesis, data are lacking on monocyte activity in type 2 diabetic patients with (DM2-MV) and without (DM2) macrovascular disease compared with control subjects. Thus, we tested whether (1) postsecretory modifications of LDL (glycation and oxidation), monocyte proatherogenic activity, and circulating levels of soluble cell adhesion molecules (sCAMs) are more pronounced in DM2-MV than in DM2 and control subjects and (2) RRR- α-tocopherol (AT) therapy, 1200 IU/d for 3 months, has a similar effect in the 3 groups (n=25 per group). Methods and Results - Although LDL glycation was increased in both diabetic groups compared with control subjects. AT therapy had no significant effect on glycation. AT therapy significantly decreased LDL oxidizability in all 3 groups. Diabetic monocytes released significantly more superoxide anion (O2 -) and interleukin-1β (IL-1β) and exhibited greater adhesion to endothelium than control subjects. AT therapy significantly decreased the release of O2 -, IL-1β, tumor necrosis factor- α, and monocyte-endothelium adhesion in all 3 groups. There was no significant difference between the 2 diabetic groups for any of the above parameters. sICAM levels were significantly elevated in both diabetic groups compared with controls. AT therapy resulted in a significant decrease in sCAMs. Conclusions - This is the first demonstration of increased IL-1β secretion and increased adhesion of monocytes to endothelium from normotriglyceridemic diabetic subjects and of decreased monocyte activity and sCAMs with AT therapy in diabetic subjects with and without macrovasculopathy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 191-196 |
| Number of pages | 6 |
| Journal | Circulation |
| Volume | 102 |
| Issue number | 2 |
| State | Published - Jul 11 2000 |
| Externally published | Yes |
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Keywords
- Cell adhesion molecules
- Cells
- Diabetes
- Proteins
- Vitamins
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
Cite this
Low-density lipoprotein postsecretory modification, monocyte function, and circulating adhesion molecules in type 2 diabetic patients with and without macrovascular complications : The effect of α-tocopherol supplementation. / Devaraj, S.; Jialal, I.
In: Circulation, Vol. 102, No. 2, 11.07.2000, p. 191-196.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Low-density lipoprotein postsecretory modification, monocyte function, and circulating adhesion molecules in type 2 diabetic patients with and without macrovascular complications
T2 - The effect of α-tocopherol supplementation
AU - Devaraj, S.
AU - Jialal, I.
PY - 2000/7/11
Y1 - 2000/7/11
N2 - Background - Although diabetes confers an increased propensity toward accelerated atherogenesis, data are lacking on monocyte activity in type 2 diabetic patients with (DM2-MV) and without (DM2) macrovascular disease compared with control subjects. Thus, we tested whether (1) postsecretory modifications of LDL (glycation and oxidation), monocyte proatherogenic activity, and circulating levels of soluble cell adhesion molecules (sCAMs) are more pronounced in DM2-MV than in DM2 and control subjects and (2) RRR- α-tocopherol (AT) therapy, 1200 IU/d for 3 months, has a similar effect in the 3 groups (n=25 per group). Methods and Results - Although LDL glycation was increased in both diabetic groups compared with control subjects. AT therapy had no significant effect on glycation. AT therapy significantly decreased LDL oxidizability in all 3 groups. Diabetic monocytes released significantly more superoxide anion (O2 -) and interleukin-1β (IL-1β) and exhibited greater adhesion to endothelium than control subjects. AT therapy significantly decreased the release of O2 -, IL-1β, tumor necrosis factor- α, and monocyte-endothelium adhesion in all 3 groups. There was no significant difference between the 2 diabetic groups for any of the above parameters. sICAM levels were significantly elevated in both diabetic groups compared with controls. AT therapy resulted in a significant decrease in sCAMs. Conclusions - This is the first demonstration of increased IL-1β secretion and increased adhesion of monocytes to endothelium from normotriglyceridemic diabetic subjects and of decreased monocyte activity and sCAMs with AT therapy in diabetic subjects with and without macrovasculopathy.
AB - Background - Although diabetes confers an increased propensity toward accelerated atherogenesis, data are lacking on monocyte activity in type 2 diabetic patients with (DM2-MV) and without (DM2) macrovascular disease compared with control subjects. Thus, we tested whether (1) postsecretory modifications of LDL (glycation and oxidation), monocyte proatherogenic activity, and circulating levels of soluble cell adhesion molecules (sCAMs) are more pronounced in DM2-MV than in DM2 and control subjects and (2) RRR- α-tocopherol (AT) therapy, 1200 IU/d for 3 months, has a similar effect in the 3 groups (n=25 per group). Methods and Results - Although LDL glycation was increased in both diabetic groups compared with control subjects. AT therapy had no significant effect on glycation. AT therapy significantly decreased LDL oxidizability in all 3 groups. Diabetic monocytes released significantly more superoxide anion (O2 -) and interleukin-1β (IL-1β) and exhibited greater adhesion to endothelium than control subjects. AT therapy significantly decreased the release of O2 -, IL-1β, tumor necrosis factor- α, and monocyte-endothelium adhesion in all 3 groups. There was no significant difference between the 2 diabetic groups for any of the above parameters. sICAM levels were significantly elevated in both diabetic groups compared with controls. AT therapy resulted in a significant decrease in sCAMs. Conclusions - This is the first demonstration of increased IL-1β secretion and increased adhesion of monocytes to endothelium from normotriglyceridemic diabetic subjects and of decreased monocyte activity and sCAMs with AT therapy in diabetic subjects with and without macrovasculopathy.
KW - Cell adhesion molecules
KW - Cells
KW - Diabetes
KW - Proteins
KW - Vitamins
UR - http://www.scopus.com/inward/record.url?scp=0034636751&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034636751&partnerID=8YFLogxK
M3 - Article
C2 - 10889130
AN - SCOPUS:0034636751
VL - 102
SP - 191
EP - 196
JO - Circulation
JF - Circulation
SN - 0009-7322
IS - 2
ER -