Loss of superfbial femoral artery relaxation following ischemia-reperfusion

Philip C. Chiang, David K. Traul, Michael M. Farooq, Robert J. Lesniak, Gary R. Seabrook, Jonathan B. Towne, Julie A. Freischlag

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Acute ischemia followed by reperfusion in skeletal muscle is associated with tissue edema and necrosis. The purpose of this study was to demonstrate superficial femoral artery endothelial injury following complete ischemia with reperfusion. New Zealand white rabbits underwent total devascularization of one hindlimb for 3 hr followed by 0, 1, and 2 hr of reperfusion. Control rabbits underwent a sham operation. Superficial femoral artery rings were then studied for acetylcholine induced relaxation in vitro. The response to acetylcholine was measured as percentage relaxation at three incremental doses (1 × 10 7, 3 × 10 7, and 5 × 10 -7 M). The ischemia-only (26.30 ± 7.07, 62.63 ± 8.64, 88.08 ± 5.25%) and the 1-hr reperfusion group (19.35 ± 12.99, 39.24 ± 15.78, 62.01 ± 14.03%) showed no significant difference (P ≥ 0.05, Student's t test) in relaxation as compared to the control group (13.73 ± 2.11, 47.88 ± 7.23, 72.44 ± 9.00%). The 2-hr reperfusion group (6.10 ± 1.02, 15.33 ± 2.56, 34.67 ± 6.31%), however, had a significant loss of relaxation at all three doses of acetylcholine compared to that seen in the control group (P ≤ 0.05, Student's t test). In this model of complete ischemia, superficial femoral artery rings lose their ability to relax in response to acetylcholine following 3 hr of ischemia and 2 hr of reperfusion, demonstrating endothelial injury. However, immediately after 3 hr of ischemia or ischemia followed by only 1 hr of reperfusion, superficial femoral artery rings did not lose their ability to relax in response to acetylcholine. This study identifies a window of opportunity for therapeutic intervention after ischemia and prior to endothelial injury from reperfusion.

Original languageEnglish (US)
Pages (from-to)361-364
Number of pages4
JournalJournal of Surgical Research
Volume60
Issue number2
DOIs
StatePublished - Feb 1 1996
Externally publishedYes

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Femoral Artery
Reperfusion
Ischemia
Acetylcholine
Students
Rabbits
Control Groups
Wounds and Injuries
Hindlimb
Reperfusion Injury
Edema
Skeletal Muscle
Necrosis

ASJC Scopus subject areas

  • Surgery

Cite this

Chiang, P. C., Traul, D. K., Farooq, M. M., Lesniak, R. J., Seabrook, G. R., Towne, J. B., & Freischlag, J. A. (1996). Loss of superfbial femoral artery relaxation following ischemia-reperfusion. Journal of Surgical Research, 60(2), 361-364. https://doi.org/10.1006/jsre.1996.0058

Loss of superfbial femoral artery relaxation following ischemia-reperfusion. / Chiang, Philip C.; Traul, David K.; Farooq, Michael M.; Lesniak, Robert J.; Seabrook, Gary R.; Towne, Jonathan B.; Freischlag, Julie A.

In: Journal of Surgical Research, Vol. 60, No. 2, 01.02.1996, p. 361-364.

Research output: Contribution to journalArticle

Chiang, PC, Traul, DK, Farooq, MM, Lesniak, RJ, Seabrook, GR, Towne, JB & Freischlag, JA 1996, 'Loss of superfbial femoral artery relaxation following ischemia-reperfusion', Journal of Surgical Research, vol. 60, no. 2, pp. 361-364. https://doi.org/10.1006/jsre.1996.0058
Chiang PC, Traul DK, Farooq MM, Lesniak RJ, Seabrook GR, Towne JB et al. Loss of superfbial femoral artery relaxation following ischemia-reperfusion. Journal of Surgical Research. 1996 Feb 1;60(2):361-364. https://doi.org/10.1006/jsre.1996.0058
Chiang, Philip C. ; Traul, David K. ; Farooq, Michael M. ; Lesniak, Robert J. ; Seabrook, Gary R. ; Towne, Jonathan B. ; Freischlag, Julie A. / Loss of superfbial femoral artery relaxation following ischemia-reperfusion. In: Journal of Surgical Research. 1996 ; Vol. 60, No. 2. pp. 361-364.
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abstract = "Acute ischemia followed by reperfusion in skeletal muscle is associated with tissue edema and necrosis. The purpose of this study was to demonstrate superficial femoral artery endothelial injury following complete ischemia with reperfusion. New Zealand white rabbits underwent total devascularization of one hindlimb for 3 hr followed by 0, 1, and 2 hr of reperfusion. Control rabbits underwent a sham operation. Superficial femoral artery rings were then studied for acetylcholine induced relaxation in vitro. The response to acetylcholine was measured as percentage relaxation at three incremental doses (1 × 10 7, 3 × 10 7, and 5 × 10 -7 M). The ischemia-only (26.30 ± 7.07, 62.63 ± 8.64, 88.08 ± 5.25{\%}) and the 1-hr reperfusion group (19.35 ± 12.99, 39.24 ± 15.78, 62.01 ± 14.03{\%}) showed no significant difference (P ≥ 0.05, Student's t test) in relaxation as compared to the control group (13.73 ± 2.11, 47.88 ± 7.23, 72.44 ± 9.00{\%}). The 2-hr reperfusion group (6.10 ± 1.02, 15.33 ± 2.56, 34.67 ± 6.31{\%}), however, had a significant loss of relaxation at all three doses of acetylcholine compared to that seen in the control group (P ≤ 0.05, Student's t test). In this model of complete ischemia, superficial femoral artery rings lose their ability to relax in response to acetylcholine following 3 hr of ischemia and 2 hr of reperfusion, demonstrating endothelial injury. However, immediately after 3 hr of ischemia or ischemia followed by only 1 hr of reperfusion, superficial femoral artery rings did not lose their ability to relax in response to acetylcholine. This study identifies a window of opportunity for therapeutic intervention after ischemia and prior to endothelial injury from reperfusion.",
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