Loss of innate host defense following unprotected vaginal sex

Natasha Nakra, Rebecca Pellett Madan, Niall Buckley, Ashley M. Huber, Jamie L. Freiermuth, Lilia Espinoza, Jennifer Walsh, Urvi M. Parikh, Kerri J. Penrose, Marla J. Keller, Betsy C. Herold

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background. Multiple host defense mechanisms protect the female genital tract from pathogens, but the impact of sexual intercourse on defense is unknown. Methods. As part of a hypothesis-generating study, 17 women provided cervicovaginal lavage (CVL) specimens at baseline (all had abstained from sexual intercourse, masturbation, and vaginal product use for 72 hours prior to screening), 2-6 hours and 10-14 hours after vaginal intercourse with a male condom, and 2-6 hours and 10-14 hours after vaginal intercourse without a male condom (5 visits total, including the baseline visit). Vaginal pH, concentrations of immune molecules, and antimicrobial activity at postcoital visits were compared to baseline values. Results. Vaginal pH and the transforming growth factor β1 level increased, but human beta-defensin 2 (HBD-2), HBD-3, and interleukin 8 levels decreased after unprotected sex. Median Escherichia coli inhibitory activity in CVL specimens decreased significantly from baseline at the visit 2-6 hours after unprotected sex (63% [range, -34% to 99%] vs 5% [range, -51% to 100%]; P = .02) and remained low at the visit 10-14 hours after unprotected sex (6% [range, -19% to 92%]; P = .02). Pooled human seminal plasma enhanced E. coli growth in vitro in a dose-dependent manner and, when added to CVL samples with high anti-E. coli activity, reversed the inhibition. Conclusions. Unprotected vaginal sex results in a reduction in endogenous anti-E. coli activity, which may reflect, in part, enhancement of bacterial growth by seminal plasma. This finding may contribute to the risk of E. coli vaginal colonization following sexual intercourse.

Original languageEnglish (US)
Pages (from-to)840-847
Number of pages8
JournalJournal of Infectious Diseases
Volume212
Issue number11
DOIs
StatePublished - 2016
Externally publishedYes

Fingerprint

Unsafe Sex
Escherichia coli
Coitus
Therapeutic Irrigation
Condoms
Semen
Masturbation
Transforming Growth Factors
Defense Mechanisms
Growth
Interleukin-8

Keywords

  • E. coli
  • Female genital tract
  • Human beta defensins
  • Mucosal immunity
  • Semen
  • Sexual intercourse

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Nakra, N., Madan, R. P., Buckley, N., Huber, A. M., Freiermuth, J. L., Espinoza, L., ... Herold, B. C. (2016). Loss of innate host defense following unprotected vaginal sex. Journal of Infectious Diseases, 212(11), 840-847. https://doi.org/10.1093/infdis/jiv488

Loss of innate host defense following unprotected vaginal sex. / Nakra, Natasha; Madan, Rebecca Pellett; Buckley, Niall; Huber, Ashley M.; Freiermuth, Jamie L.; Espinoza, Lilia; Walsh, Jennifer; Parikh, Urvi M.; Penrose, Kerri J.; Keller, Marla J.; Herold, Betsy C.

In: Journal of Infectious Diseases, Vol. 212, No. 11, 2016, p. 840-847.

Research output: Contribution to journalArticle

Nakra, N, Madan, RP, Buckley, N, Huber, AM, Freiermuth, JL, Espinoza, L, Walsh, J, Parikh, UM, Penrose, KJ, Keller, MJ & Herold, BC 2016, 'Loss of innate host defense following unprotected vaginal sex', Journal of Infectious Diseases, vol. 212, no. 11, pp. 840-847. https://doi.org/10.1093/infdis/jiv488
Nakra N, Madan RP, Buckley N, Huber AM, Freiermuth JL, Espinoza L et al. Loss of innate host defense following unprotected vaginal sex. Journal of Infectious Diseases. 2016;212(11):840-847. https://doi.org/10.1093/infdis/jiv488
Nakra, Natasha ; Madan, Rebecca Pellett ; Buckley, Niall ; Huber, Ashley M. ; Freiermuth, Jamie L. ; Espinoza, Lilia ; Walsh, Jennifer ; Parikh, Urvi M. ; Penrose, Kerri J. ; Keller, Marla J. ; Herold, Betsy C. / Loss of innate host defense following unprotected vaginal sex. In: Journal of Infectious Diseases. 2016 ; Vol. 212, No. 11. pp. 840-847.
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abstract = "Background. Multiple host defense mechanisms protect the female genital tract from pathogens, but the impact of sexual intercourse on defense is unknown. Methods. As part of a hypothesis-generating study, 17 women provided cervicovaginal lavage (CVL) specimens at baseline (all had abstained from sexual intercourse, masturbation, and vaginal product use for 72 hours prior to screening), 2-6 hours and 10-14 hours after vaginal intercourse with a male condom, and 2-6 hours and 10-14 hours after vaginal intercourse without a male condom (5 visits total, including the baseline visit). Vaginal pH, concentrations of immune molecules, and antimicrobial activity at postcoital visits were compared to baseline values. Results. Vaginal pH and the transforming growth factor β1 level increased, but human beta-defensin 2 (HBD-2), HBD-3, and interleukin 8 levels decreased after unprotected sex. Median Escherichia coli inhibitory activity in CVL specimens decreased significantly from baseline at the visit 2-6 hours after unprotected sex (63{\%} [range, -34{\%} to 99{\%}] vs 5{\%} [range, -51{\%} to 100{\%}]; P = .02) and remained low at the visit 10-14 hours after unprotected sex (6{\%} [range, -19{\%} to 92{\%}]; P = .02). Pooled human seminal plasma enhanced E. coli growth in vitro in a dose-dependent manner and, when added to CVL samples with high anti-E. coli activity, reversed the inhibition. Conclusions. Unprotected vaginal sex results in a reduction in endogenous anti-E. coli activity, which may reflect, in part, enhancement of bacterial growth by seminal plasma. This finding may contribute to the risk of E. coli vaginal colonization following sexual intercourse.",
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