Longitudinal in vivo imaging to assess blood flow and oxygenation in implantable engineered tissues

Sean M. White, Ryan Hingorani, Rajan Arora, Christopher C.W. Hughes, Steven George, Bernard Choi

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The functionality of vascular networks within implanted prevascularized tissues is difficult to assess using traditional analysis techniques, such as histology. This is largely due to the inability to visualize hemodynamics in a longitudinal fashion. To address this limitation, we developed dynamic imaging methods to measure blood flow and hemoglobin oxygen saturation in implanted prevascularized tissues noninvasively and longitudinally. Using laser speckle imaging, multispectral imaging, and intravital microscopy, we demonstrate that fibrin-based tissue implants anastomose with the host (severe combined immunodeficient mice) in as short as 20 h. Anastomosis results in initial perfusion with highly oxygenated blood, and an increase in average hemoglobin oxygenation of 53%. However, shear rates in the preformed vessels were low (20.8 ± 12.8 s -1), and flow did not persist in the vast majority of preformed vessels due to thrombus formation. These findings suggest that designing an appropriate vascular network structure in prevascularized tissues to maintain shear rates above the threshold for thrombosis may be necessary to maintain flow following implantation. We conclude that wide-field and microscopic functional imaging together enables longitudinal assessment of hemodynamics in prevascularized tissues, and rapid evaluation and refinement of vascularization strategies used in tissue engineering.

Original languageEnglish (US)
Title of host publicationFrontiers in Optics, FIO 2012
StatePublished - Dec 1 2012
EventFrontiers in Optics, FIO 2012 - Rochester, NY, United States
Duration: Oct 14 2012Oct 18 2012


OtherFrontiers in Optics, FIO 2012
Country/TerritoryUnited States
CityRochester, NY

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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