Abstract
The functionality of vascular networks within implanted prevascularized tissues is difficult to assess using traditional analysis techniques, such as histology. This is largely due to the inability to visualize hemodynamics in a longitudinal fashion. To address this limitation, we developed dynamic imaging methods to measure blood flow and hemoglobin oxygen saturation in implanted prevascularized tissues noninvasively and longitudinally. Using laser speckle imaging, multispectral imaging, and intravital microscopy, we demonstrate that fibrin-based tissue implants anastomose with the host (severe combined immunodeficient mice) in as short as 20 h. Anastomosis results in initial perfusion with highly oxygenated blood, and an increase in average hemoglobin oxygenation of 53%. However, shear rates in the preformed vessels were low (20.8 ± 12.8 s -1), and flow did not persist in the vast majority of preformed vessels due to thrombus formation. These findings suggest that designing an appropriate vascular network structure in prevascularized tissues to maintain shear rates above the threshold for thrombosis may be necessary to maintain flow following implantation. We conclude that wide-field and microscopic functional imaging together enables longitudinal assessment of hemodynamics in prevascularized tissues, and rapid evaluation and refinement of vascularization strategies used in tissue engineering.
| Original language | English (US) |
|---|---|
| Title of host publication | Frontiers in Optics, FIO 2012 |
| State | Published - Dec 1 2012 |
| Event | Frontiers in Optics, FIO 2012 - Rochester, NY, United States Duration: Oct 14 2012 → Oct 18 2012 |
Other
| Other | Frontiers in Optics, FIO 2012 |
|---|---|
| Country | United States |
| City | Rochester, NY |
| Period | 10/14/12 → 10/18/12 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Longitudinal in vivo imaging to assess blood flow and oxygenation in implantable engineered tissues. / White, Sean M.; Hingorani, Ryan; Arora, Rajan; Hughes, Christopher C.W.; George, Steven; Choi, Bernard.
Frontiers in Optics, FIO 2012. 2012.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Longitudinal in vivo imaging to assess blood flow and oxygenation in implantable engineered tissues
AU - White, Sean M.
AU - Hingorani, Ryan
AU - Arora, Rajan
AU - Hughes, Christopher C.W.
AU - George, Steven
AU - Choi, Bernard
PY - 2012/12/1
Y1 - 2012/12/1
N2 - The functionality of vascular networks within implanted prevascularized tissues is difficult to assess using traditional analysis techniques, such as histology. This is largely due to the inability to visualize hemodynamics in a longitudinal fashion. To address this limitation, we developed dynamic imaging methods to measure blood flow and hemoglobin oxygen saturation in implanted prevascularized tissues noninvasively and longitudinally. Using laser speckle imaging, multispectral imaging, and intravital microscopy, we demonstrate that fibrin-based tissue implants anastomose with the host (severe combined immunodeficient mice) in as short as 20 h. Anastomosis results in initial perfusion with highly oxygenated blood, and an increase in average hemoglobin oxygenation of 53%. However, shear rates in the preformed vessels were low (20.8 ± 12.8 s -1), and flow did not persist in the vast majority of preformed vessels due to thrombus formation. These findings suggest that designing an appropriate vascular network structure in prevascularized tissues to maintain shear rates above the threshold for thrombosis may be necessary to maintain flow following implantation. We conclude that wide-field and microscopic functional imaging together enables longitudinal assessment of hemodynamics in prevascularized tissues, and rapid evaluation and refinement of vascularization strategies used in tissue engineering.
AB - The functionality of vascular networks within implanted prevascularized tissues is difficult to assess using traditional analysis techniques, such as histology. This is largely due to the inability to visualize hemodynamics in a longitudinal fashion. To address this limitation, we developed dynamic imaging methods to measure blood flow and hemoglobin oxygen saturation in implanted prevascularized tissues noninvasively and longitudinally. Using laser speckle imaging, multispectral imaging, and intravital microscopy, we demonstrate that fibrin-based tissue implants anastomose with the host (severe combined immunodeficient mice) in as short as 20 h. Anastomosis results in initial perfusion with highly oxygenated blood, and an increase in average hemoglobin oxygenation of 53%. However, shear rates in the preformed vessels were low (20.8 ± 12.8 s -1), and flow did not persist in the vast majority of preformed vessels due to thrombus formation. These findings suggest that designing an appropriate vascular network structure in prevascularized tissues to maintain shear rates above the threshold for thrombosis may be necessary to maintain flow following implantation. We conclude that wide-field and microscopic functional imaging together enables longitudinal assessment of hemodynamics in prevascularized tissues, and rapid evaluation and refinement of vascularization strategies used in tissue engineering.
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M3 - Conference contribution
AN - SCOPUS:84893073374
SN - 9781557529565
BT - Frontiers in Optics, FIO 2012
ER -