Long-term study of cell-mediated responses to Borrelia burgdorferi in the laboratory mouse

M. S. De Souza, A. L. Smith, D. S. Beck, G. A. Terwilliger, E. Fikrig, Stephen W Barthold

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Borrelia burgdorferi infection of disease-susceptible (C3H) and -resistant (BALB) mice resulted in impaired proliferation to both T- and B-cell mitogens up to 30 days after inoculation. Interleukin-2 and -4 production was also impaired, paralleling the T-cell response to concanavalin A. Impaired lymphocyte proliferation could not be attributed to diminished numbers of T or B cells and was found to depend on the lymphoid organ (spleen or lymph node) examined. Prostaglandin production accounted for part of this immune dysfunction. Attempts to assess antigen-specific proliferation to B. burgdorferi were inconsistent, and delayed-type hypersensitivity responses were not detected. Adoptive transfer of T-enriched cells from chronically infected donors failed to prevent infection and disease development in recipient C3H mice. The current study emphasizes caution in the study of B. burgdorferi antigen-specific assays and argues against the role of a vigorous T-cell response in Lyme borreliosis in infected laboratory mice.

Original languageEnglish (US)
Pages (from-to)1814-1822
Number of pages9
JournalInfection and Immunity
Volume61
Issue number5
StatePublished - 1993
Externally publishedYes

Fingerprint

Borrelia burgdorferi
T-Lymphocytes
B-Lymphocytes
Borrelia Infections
Antigens
Lyme Disease
Adoptive Transfer
Inbred C3H Mouse
Delayed Hypersensitivity
Concanavalin A
Mitogens
Interleukin-4
Prostaglandins
Interleukin-2
Spleen
Lymph Nodes
Lymphocytes
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

De Souza, M. S., Smith, A. L., Beck, D. S., Terwilliger, G. A., Fikrig, E., & Barthold, S. W. (1993). Long-term study of cell-mediated responses to Borrelia burgdorferi in the laboratory mouse. Infection and Immunity, 61(5), 1814-1822.

Long-term study of cell-mediated responses to Borrelia burgdorferi in the laboratory mouse. / De Souza, M. S.; Smith, A. L.; Beck, D. S.; Terwilliger, G. A.; Fikrig, E.; Barthold, Stephen W.

In: Infection and Immunity, Vol. 61, No. 5, 1993, p. 1814-1822.

Research output: Contribution to journalArticle

De Souza, MS, Smith, AL, Beck, DS, Terwilliger, GA, Fikrig, E & Barthold, SW 1993, 'Long-term study of cell-mediated responses to Borrelia burgdorferi in the laboratory mouse', Infection and Immunity, vol. 61, no. 5, pp. 1814-1822.
De Souza, M. S. ; Smith, A. L. ; Beck, D. S. ; Terwilliger, G. A. ; Fikrig, E. ; Barthold, Stephen W. / Long-term study of cell-mediated responses to Borrelia burgdorferi in the laboratory mouse. In: Infection and Immunity. 1993 ; Vol. 61, No. 5. pp. 1814-1822.
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