Long term Raman spectral study of power-dependent photodamage in red blood cells

Marcos A S De Oliveira, Zachary J. Smith, Florian Knorr, Renato E. De Araujo, Sebastian Wachsmann-Hogiu

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We monitored time-dependent changes in the Raman spectra of optically trapped red blood cells. By fitting the Raman peaks of individual spectra over time, high-precision time evolutions of peak positions and intensities were obtained. These changes are dependent on the trapping laser power. Characteristic times for these changes were determined for each laser power by fitting the time courses with multi-exponential curves. Raman spectral dynamics showed significant and irreversible changes as a function of trapping duration that we attribute to a combination of photodamage of hemoglobin at short times followed by diffusion of hemoglobin out of the cell at longer times.

Original languageEnglish (US)
Article number103702
JournalApplied Physics Letters
Volume104
Issue number10
DOIs
StatePublished - Mar 10 2014

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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