Long-term morphologic and biochemical features of experimentally induced lung fibrosis in the mouse

W. M. Haschek, A. J P Klein-Szanto, Jerold A Last, K. M. Reiser, H. Witschi

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Mice were injected with 400 mg per kg of butylated hydroxytoluene (BHT) and exposed to an atmosphere of 70 per cent oxygen for 6 days. Control groups were animals treated with BHT alone or injected with vehicle and kept for 6 days in either O2 or air. Animals were killed at various time intervals after BHT treatment, and the lung changes were evaluated with biochemical and morphologic techniques. In animals exposed to BHT + O2, there was initially a diffuse interstitial pneumonitis, increased levels of interstitial collagen, and a decreased ratio of type III to type I collagen. With time the inflammatory component subsided, and degenerative changes primarily consisting of dilation of terminal airways and of alveoli became apparent. Elevated levels of lung hydroxyproline persisted until the termination of the study at 1 year, although ratios of type III to type I collagen returned to normal. In animals treated with BHT alone, collagen accumulation and morphologic changes were similar, although much less severe. It is concluded that potentiation of acute lung injury by oxygen produces long-lasting morphologic and biochemical alterations in lung parenchymal.

Original languageEnglish (US)
Pages (from-to)438-449
Number of pages12
JournalLaboratory Investigation
Volume46
Issue number4
StatePublished - 1982
Externally publishedYes

Fingerprint

Butylated Hydroxytoluene
Fibrosis
Lung
Collagen Type I
Collagen
Oxygen
Acute Lung Injury
Hydroxyproline
Interstitial Lung Diseases
Atmosphere
Dilatation
Air
Control Groups

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Haschek, W. M., Klein-Szanto, A. J. P., Last, J. A., Reiser, K. M., & Witschi, H. (1982). Long-term morphologic and biochemical features of experimentally induced lung fibrosis in the mouse. Laboratory Investigation, 46(4), 438-449.

Long-term morphologic and biochemical features of experimentally induced lung fibrosis in the mouse. / Haschek, W. M.; Klein-Szanto, A. J P; Last, Jerold A; Reiser, K. M.; Witschi, H.

In: Laboratory Investigation, Vol. 46, No. 4, 1982, p. 438-449.

Research output: Contribution to journalArticle

Haschek, WM, Klein-Szanto, AJP, Last, JA, Reiser, KM & Witschi, H 1982, 'Long-term morphologic and biochemical features of experimentally induced lung fibrosis in the mouse', Laboratory Investigation, vol. 46, no. 4, pp. 438-449.
Haschek, W. M. ; Klein-Szanto, A. J P ; Last, Jerold A ; Reiser, K. M. ; Witschi, H. / Long-term morphologic and biochemical features of experimentally induced lung fibrosis in the mouse. In: Laboratory Investigation. 1982 ; Vol. 46, No. 4. pp. 438-449.
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