Long-term maintenance of a microfluidic 3D human liver sinusoid

Ljupcho Prodanov, Rohit Jindal, Shyam Sundhar Bale, Manjunath Hegde, William J. Mccarty, Inna Golberg, Abhinav Bhushan, Martin L. Yarmush, Osman Berk Usta

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

The development of long-term human organotypic liver-on-a-chip models for successful prediction of toxic response is one of the most important and urgent goals of the NIH/DARPA's initiative to replicate and replace chronic and acute drug testing in animals. For this purpose, we developed a microfluidic chip that consists of two microfluidic chambers separated by a porous membrane. The aim of this communication is to demonstrate the recapitulation of a liver sinusoid-on-a-chip, using human cells only for a period of 28 days. Using a step-by-step method for building a 3D microtissue on-a-chip, we demonstrate that an organotypic in vitro model that reassembles the liver sinusoid microarchitecture can be maintained successfully for a period of 28 days. In addition, higher albumin synthesis (synthetic) and urea excretion (detoxification) were observed under flow compared to static cultures. This human liver-on-a-chip should be further evaluated in drug-related studies.

Original languageEnglish (US)
Pages (from-to)241-246
Number of pages6
JournalBiotechnology and Bioengineering
Volume113
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Keywords

  • 3D microtissue
  • Hepatocytes
  • Liver sinusoid
  • Liver-on-a-chip
  • Microfludics

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • Cite this

    Prodanov, L., Jindal, R., Bale, S. S., Hegde, M., Mccarty, W. J., Golberg, I., Bhushan, A., Yarmush, M. L., & Usta, O. B. (2016). Long-term maintenance of a microfluidic 3D human liver sinusoid. Biotechnology and Bioengineering, 113(1), 241-246. https://doi.org/10.1002/bit.25700