Long-term kinetic study of β-carotene, using accelerator mass spectrometry in an adult volunteer

S. R. Dueker, Y. Lin, B. A. Buchholz, Philip D Schneider, M. W. Lame, H. J. Segall, J. S. Vogel, A. J. Clifford

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

We present a sensitive tracer method, suitable for in vivo human research, that uses β-[14C]carotene coupled with accelerator mass spectrometry (AMS) detection. Using this approach, the concentration-time course of a physiological (306 μg; 200 nCi) oral dose of β-[14C]carotene was determined for 209 days in plasma. Analytes included β-[14C]carotene, [14C]retinyl esters, [14C]retinol, and several [14C]retinoic acids. There was a 5.5-h lag between dosing and the appearance of 14C in plasma. Labeled β-carotene and [14C]retinyl esters rose and displayed several maxima with virtually identical kinetic profiles over the first 24-h period; elevated [14C]retinyl ester concentrations were sustained in the plasma compartment for >21 h postdosing. The appearance of [14C]retinol in plasma was also delayed 5.5 h postdosing and its concentration rose linearly for 28 h before declining. Cumulative urine and stool were collected for 17 and 10 days, respectively, and 57.4% of the dose was recovered in the stool within 48 h postdosing. The stool was the major excretion route for the absorbed dose. The turnover times (1/k(el)) for β-carotene and retinol were 58 and 302 days, respectively. Area under the curve analysis of the plasma response curves suggested a molar vitamin A value of 0.53 for β-carotene, with a minimum of 62% of the absorbed β-carotene being cleaved to vitamin A. In summary, AMS is an excellent tool for defining the in vivo metabolic behavior of β-carotene and related compounds at physiological concentrations. Further, our data suggest that retinyl esters derived from β-carotene may undergo hepatic resecretion with VLDL in a process similar to that observed for β-carotene.

Original languageEnglish (US)
Pages (from-to)1790-1800
Number of pages11
JournalJournal of Lipid Research
Volume41
Issue number11
StatePublished - 2000

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Carotenoids
Particle accelerators
Mass spectrometry
Volunteers
Mass Spectrometry
Kinetics
Vitamin A
Plasmas
Esters
Tretinoin
Area Under Curve
Urine
Liver

Keywords

  • Human
  • Isotope
  • Vitamin A

ASJC Scopus subject areas

  • Endocrinology

Cite this

Dueker, S. R., Lin, Y., Buchholz, B. A., Schneider, P. D., Lame, M. W., Segall, H. J., ... Clifford, A. J. (2000). Long-term kinetic study of β-carotene, using accelerator mass spectrometry in an adult volunteer. Journal of Lipid Research, 41(11), 1790-1800.

Long-term kinetic study of β-carotene, using accelerator mass spectrometry in an adult volunteer. / Dueker, S. R.; Lin, Y.; Buchholz, B. A.; Schneider, Philip D; Lame, M. W.; Segall, H. J.; Vogel, J. S.; Clifford, A. J.

In: Journal of Lipid Research, Vol. 41, No. 11, 2000, p. 1790-1800.

Research output: Contribution to journalArticle

Dueker, SR, Lin, Y, Buchholz, BA, Schneider, PD, Lame, MW, Segall, HJ, Vogel, JS & Clifford, AJ 2000, 'Long-term kinetic study of β-carotene, using accelerator mass spectrometry in an adult volunteer', Journal of Lipid Research, vol. 41, no. 11, pp. 1790-1800.
Dueker, S. R. ; Lin, Y. ; Buchholz, B. A. ; Schneider, Philip D ; Lame, M. W. ; Segall, H. J. ; Vogel, J. S. ; Clifford, A. J. / Long-term kinetic study of β-carotene, using accelerator mass spectrometry in an adult volunteer. In: Journal of Lipid Research. 2000 ; Vol. 41, No. 11. pp. 1790-1800.
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abstract = "We present a sensitive tracer method, suitable for in vivo human research, that uses β-[14C]carotene coupled with accelerator mass spectrometry (AMS) detection. Using this approach, the concentration-time course of a physiological (306 μg; 200 nCi) oral dose of β-[14C]carotene was determined for 209 days in plasma. Analytes included β-[14C]carotene, [14C]retinyl esters, [14C]retinol, and several [14C]retinoic acids. There was a 5.5-h lag between dosing and the appearance of 14C in plasma. Labeled β-carotene and [14C]retinyl esters rose and displayed several maxima with virtually identical kinetic profiles over the first 24-h period; elevated [14C]retinyl ester concentrations were sustained in the plasma compartment for >21 h postdosing. The appearance of [14C]retinol in plasma was also delayed 5.5 h postdosing and its concentration rose linearly for 28 h before declining. Cumulative urine and stool were collected for 17 and 10 days, respectively, and 57.4{\%} of the dose was recovered in the stool within 48 h postdosing. The stool was the major excretion route for the absorbed dose. The turnover times (1/k(el)) for β-carotene and retinol were 58 and 302 days, respectively. Area under the curve analysis of the plasma response curves suggested a molar vitamin A value of 0.53 for β-carotene, with a minimum of 62{\%} of the absorbed β-carotene being cleaved to vitamin A. In summary, AMS is an excellent tool for defining the in vivo metabolic behavior of β-carotene and related compounds at physiological concentrations. Further, our data suggest that retinyl esters derived from β-carotene may undergo hepatic resecretion with VLDL in a process similar to that observed for β-carotene.",
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AU - Schneider, Philip D

AU - Lame, M. W.

AU - Segall, H. J.

AU - Vogel, J. S.

AU - Clifford, A. J.

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