Long term exposure effect of a unique metabolic nutrition system containing a diverse group of phytochemicals on serum chemistry and genomic and non-genomic changes in the liver of female B6C3F1 mice

Sidhartha D. Ray, Mayur Parmar, Ismail Syed, Jasmine Rathod, Daniel Zinkovsky, Elida Bulku, Joseph Gigliorti, Robert M. Hackman, Sidney J. Stohs

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The nutritional value and therapeutic benefits coupled with presumed safety have heightened interest in the use of custom designed dietary supplements. Their use has increased substantially since the passage of the 1994 Dietary Supplement Health Education Act. However, few well-controlled studies have been conducted to assess the safety and potential adverse effects of dietary supplements. MNSO (MNS Orange-AdvoCare®) is a unique combination of vitamins, minerals, omega-3 fatty acids and herbal extracts designed to provide a strong foundation of nutritional support, enhance thermogenesis, heighten energy levels and improve immune status. This investigation was designed to explore the safety and toxic effects, if any, of 12 months of continuous exposure to the ephedra and caffeine containing MNSO on serum chemistry and histopathology of seven vital target organs in female B6C3F1 mice. MNSO is enriched with extracts of citrus, Ephedra, Ginkgo, green tea and Ocimum. In this study, mice were fed control (-MNSO) or MNSO (1x-10x, 1x = daily human dose) diets. Blood was collected from all groups including the control every 4 months for serum chemistry analysis (enzyme, lipid, carbohydrate, electrolyte profiles), and liver tissue was collected for tissue biochemistry and histopathology. Multiple biochemical parameters included: (i) determination of oxidative stress via lipid peroxidation and (ii) assessment of genomic integrity via DNA fragmentation. In addition, food consumption and body weight changes were also monitored biweekly. The data showed that 12 months ingestion of 10x-MNSO did not significantly influence organ histopathology, alter the normal serum chemistry profile or any of the crucial tissue biochemistry. MNSO-exposed animals were more active, consumed more food and were relatively leaner compared with the controls. This study indicates that a caffeine and ephedra containing metabolic nutrition system is non-toxic and non-hepatotoxic in mice at up to 10x the human consumption dose of ephedra.

Original languageEnglish (US)
Pages (from-to)458-471
Number of pages14
JournalPhytotherapy Research
Volume22
Issue number4
DOIs
StatePublished - Apr 2008

Fingerprint

Dietary supplements
Phytochemicals
Nutrition
Liver
Ephedra
Biochemistry
Tissue
Caffeine
Serum
Dietary Supplements
Lipids
Oxidative stress
Poisons
Omega-3 Fatty Acids
Vitamins
Electron energy levels
Electrolytes
Minerals
Safety
Animals

Keywords

  • Cholesterol
  • DNA fragmentation
  • HDL
  • LDL
  • Liver
  • Metabolic nutrition system
  • MNSO
  • Nutraceutical
  • Oxidative stress
  • Phytochemical

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Long term exposure effect of a unique metabolic nutrition system containing a diverse group of phytochemicals on serum chemistry and genomic and non-genomic changes in the liver of female B6C3F1 mice. / Ray, Sidhartha D.; Parmar, Mayur; Syed, Ismail; Rathod, Jasmine; Zinkovsky, Daniel; Bulku, Elida; Gigliorti, Joseph; Hackman, Robert M.; Stohs, Sidney J.

In: Phytotherapy Research, Vol. 22, No. 4, 04.2008, p. 458-471.

Research output: Contribution to journalArticle

Ray, Sidhartha D. ; Parmar, Mayur ; Syed, Ismail ; Rathod, Jasmine ; Zinkovsky, Daniel ; Bulku, Elida ; Gigliorti, Joseph ; Hackman, Robert M. ; Stohs, Sidney J. / Long term exposure effect of a unique metabolic nutrition system containing a diverse group of phytochemicals on serum chemistry and genomic and non-genomic changes in the liver of female B6C3F1 mice. In: Phytotherapy Research. 2008 ; Vol. 22, No. 4. pp. 458-471.
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