Long-term cultures of human adult Schwann cells isolated from autopsy materials

G. Moretto, S. U. Kim, D. H. Shin, David E Pleasure, N. Rizzuro

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Enriched populations of adult human Schwann cells were otained from trigeminal ganglia and roots of autopsy material. The cells, isolated by enzymatic procedure, were seeded on rat tail collagencoated coverslips. Subcultures were established several weeks later, and secondary cells were grown on polylysine-coated coverslips and maintained in vitro for as long as 5 months. The Schwann cells in culture displayed the same light- and electron-microscopic features and arrangement as those cells observed in vivo. The addition of bovine pituitary glial growth factor in the medium induced a 3-5-fold increase in Schwann cell division.

Original languageEnglish (US)
Pages (from-to)15-21
Number of pages7
JournalActa Neuropathologica
Volume64
Issue number1
DOIs
StatePublished - Mar 1984
Externally publishedYes

Keywords

  • Autopsy
  • Glial growth factor
  • Human
  • Morphology
  • Schwann cells
  • Tissue culture
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pathology and Forensic Medicine
  • Clinical Neurology

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