Long-term culture of oligodendrocytes isolated from rat corpus callosum by percoll density gradient. Lysis by polyclonal antigalactocerebroside serum

M. Hirayama, D. H. Silberberg, R. P. Lisak, David E Pleasure

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Abstract

Oligodendrocytes isolated from the corpus callosum of four-week-old rats by trypsinization and Percoll density gradient centrifugation were cultured on poly-1-lysine coated coverslips. Some cells extended short processes within 24 hours (h), and at that time up to 95% of the cells showed surface binding of rabbit antiserum to galactocerebroside (anti-GalC) as demonstrated by indirect immunofluorescence. Oligodendrocytes survived up to two months in culture, extending processes with membranous elaborations. Exposure of living oligodendrocytes to varying dilutions of rabbit anti-GalC serum in the presence of complement produced cytotoxicity which was directly proportional to the concentration of antiserum and duration of exposure, as assessed by a nigrosin dye exclusion test. This system of isolating and culturing rat oligodendrocytes will permit further development and immunologic studies related to demyelinating diseases.

Original languageEnglish (US)
Pages (from-to)16-28
Number of pages13
JournalJournal of Neuropathology and Experimental Neurology
Volume42
Issue number1
StatePublished - 1983
Externally publishedYes

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

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