Abstract
The understanding of pathways associated with differentiated function in human epithelial cells has been enhanced by the development of methods for the short-term culture of human epithelial cells. In general these methods involve the use of serum. The subculture and maintenance of epithelial cells in long-term culture has been more problematic. A serum-free medium developed for human bronchial epithelial cells was slightly modified and found to be useful for the subculture and long-term maintenance of not only bronchial epithelial cells, but also tracheal, nasal polyp, and sweat gland epithelial cells from either normal or cystic fibrosis individuals. The cells maintained epithelial-specific characteristics after multiple subcultures. Monolayers of epithelial cells showed junctional complex formation, the presence of keratin, and micro villi. Functional studies with Ussing chambers showed short circuit current (Isc) responses to isoproterenol, bradykinin, or calcium ionophore (A23187) in subcultured tracheal and bronchial cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 411-418 |
| Number of pages | 8 |
| Journal | In Vitro Cellular & Developmental Biology |
| Volume | 26 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1990 |
| Externally published | Yes |
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Keywords
- cystic fibrosis
- human epithelial differentiation
- subculture
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
- Clinical Biochemistry
Cite this
Long-term culture of normal and cystic fibrosis epithelial cells grown under serum-free conditions. / Gruenert, Dieter C.; Basbaum, Carol B.; Widdicombe, Jonathan.
In: In Vitro Cellular & Developmental Biology, Vol. 26, No. 4, 04.1990, p. 411-418.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Long-term culture of normal and cystic fibrosis epithelial cells grown under serum-free conditions
AU - Gruenert, Dieter C.
AU - Basbaum, Carol B.
AU - Widdicombe, Jonathan
PY - 1990/4
Y1 - 1990/4
N2 - The understanding of pathways associated with differentiated function in human epithelial cells has been enhanced by the development of methods for the short-term culture of human epithelial cells. In general these methods involve the use of serum. The subculture and maintenance of epithelial cells in long-term culture has been more problematic. A serum-free medium developed for human bronchial epithelial cells was slightly modified and found to be useful for the subculture and long-term maintenance of not only bronchial epithelial cells, but also tracheal, nasal polyp, and sweat gland epithelial cells from either normal or cystic fibrosis individuals. The cells maintained epithelial-specific characteristics after multiple subcultures. Monolayers of epithelial cells showed junctional complex formation, the presence of keratin, and micro villi. Functional studies with Ussing chambers showed short circuit current (Isc) responses to isoproterenol, bradykinin, or calcium ionophore (A23187) in subcultured tracheal and bronchial cells.
AB - The understanding of pathways associated with differentiated function in human epithelial cells has been enhanced by the development of methods for the short-term culture of human epithelial cells. In general these methods involve the use of serum. The subculture and maintenance of epithelial cells in long-term culture has been more problematic. A serum-free medium developed for human bronchial epithelial cells was slightly modified and found to be useful for the subculture and long-term maintenance of not only bronchial epithelial cells, but also tracheal, nasal polyp, and sweat gland epithelial cells from either normal or cystic fibrosis individuals. The cells maintained epithelial-specific characteristics after multiple subcultures. Monolayers of epithelial cells showed junctional complex formation, the presence of keratin, and micro villi. Functional studies with Ussing chambers showed short circuit current (Isc) responses to isoproterenol, bradykinin, or calcium ionophore (A23187) in subcultured tracheal and bronchial cells.
KW - cystic fibrosis
KW - human epithelial differentiation
KW - subculture
UR - http://www.scopus.com/inward/record.url?scp=0025346025&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025346025&partnerID=8YFLogxK
U2 - 10.1007/BF02623833
DO - 10.1007/BF02623833
M3 - Article
C2 - 1693142
AN - SCOPUS:0025346025
VL - 26
SP - 411
EP - 418
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
SN - 1054-5476
IS - 4
ER -