Long-term culture of human oligodendrocytes. Isolation, growth and identification

Seung U. Kim, Yuji Sato, Donald H. Silberberg, David E Pleasure, Lucy B. Rorke

Research output: Contribution to journalArticlepeer-review

82 Scopus citations


Oligodendrocytes were isolated from adult human brains obtained at autopsy. The cells were prepared by Percoll density gradient centrifugation, seeded on plastic coverslips and were cultured for a period up to 6 months. The oligodendrocytes in culture expressed cell-type specific markers, galactocerebroside and myelin basic protein and revealed the ultrastructure of mature oligodendrocytes.

Original languageEnglish (US)
Pages (from-to)295-301
Number of pages7
JournalJournal of the Neurological Sciences
Issue number1-3
StatePublished - 1983
Externally publishedYes


  • Galactocerebroside
  • Human brains
  • Myelin basic protein
  • Oligodendrocytes

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology


Dive into the research topics of 'Long-term culture of human oligodendrocytes. Isolation, growth and identification'. Together they form a unique fingerprint.

Cite this