Long-term culture of human oligodendrocytes. Isolation, growth and identification

Seung U. Kim, Yuji Sato, Donald H. Silberberg, David E Pleasure, Lucy B. Rorke

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

Oligodendrocytes were isolated from adult human brains obtained at autopsy. The cells were prepared by Percoll density gradient centrifugation, seeded on plastic coverslips and were cultured for a period up to 6 months. The oligodendrocytes in culture expressed cell-type specific markers, galactocerebroside and myelin basic protein and revealed the ultrastructure of mature oligodendrocytes.

Original languageEnglish (US)
Pages (from-to)295-301
Number of pages7
JournalJournal of the Neurological Sciences
Volume62
Issue number1-3
DOIs
StatePublished - 1983
Externally publishedYes

Keywords

  • Galactocerebroside
  • Human brains
  • Myelin basic protein
  • Oligodendrocytes

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

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