Long-term culture of bovine trophoblastic cells

Linda Munson, Suzanne K. Chandler, Donald H. Schlafer

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Procedures for the isolation and cultivation of bovine cotyledonary trophoblastic cells from early second trimester placentas are described. Collagenase digestion yielded a single cell suspension of both uninucleate and binucleate cells with minimal contamination with other cell types. Optimal growth conditions were obtained through supplementation of medium with epidermal growth factor, insulin, transferrin, and selenium. Trophoblastic cells survived multiple passages, cryopreservation, and serum deprivation and have been maintained in culture for more than 14 mo. Two cell types were identified by phase microscopy: uninucleated trophoblastic cells and trophoblastic giant cells, which were predominantly binucleate cells. Binucleate cells were present in small numbers through all passages. This procedure provides a reliable method to obtain trophoblastic cell lines for studies of trophoblastic cell physiology and susceptibility to infectious and toxic agents.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalJournal of Tissue Culture Methods
Volume11
Issue number3
DOIs
StatePublished - Sep 1988
Externally publishedYes

Fingerprint

Poisons
Insulin
Physiology
Selenium
Collagenases
Transferrin
Epidermal Growth Factor
Suspensions
Microscopic examination
Contamination
Cells
Cell Physiological Phenomena
Cryopreservation
Second Pregnancy Trimester
Giant Cells
Placenta
Digestion
Microscopy
Cell Line
Growth

Keywords

  • bovine
  • placenta
  • tissue culture
  • trophoblastic cell

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Cite this

Long-term culture of bovine trophoblastic cells. / Munson, Linda; Chandler, Suzanne K.; Schlafer, Donald H.

In: Journal of Tissue Culture Methods, Vol. 11, No. 3, 09.1988, p. 123-128.

Research output: Contribution to journalArticle

Munson, L, Chandler, SK & Schlafer, DH 1988, 'Long-term culture of bovine trophoblastic cells', Journal of Tissue Culture Methods, vol. 11, no. 3, pp. 123-128. https://doi.org/10.1007/BF01404264
Munson, Linda ; Chandler, Suzanne K. ; Schlafer, Donald H. / Long-term culture of bovine trophoblastic cells. In: Journal of Tissue Culture Methods. 1988 ; Vol. 11, No. 3. pp. 123-128.
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