Abstract
Oligodendroglia were isolated from calf central nervous system (CNS) white matter by trypsinization in phosphate buffered saline and separation by centrifugation through Percoll. Using antisera to phenotypic markers and double labelling experiments we were able to identify essentially all cells in the cultures. The cells obtained were: (1) viable; (2) had intact plasma membranes and well preserved organelles, ribosomes and mitochondria; and (3) were ≥95% oligodendroglia 16-20 h after isolation as determined by ability to bind antigalactocerebroside antibodies (anti-GalC). Oligodendroglia could be cultured for several weeks to months. Oligodendroglia established and maintained processes which bound anti-GalC. Myelin basic protein could be demonstrated in the cytoplasm of 40-60% of oligodendroglia cell bodies but not in the processes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 107-122 |
| Number of pages | 16 |
| Journal | Brain Research |
| Volume | 223 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 26 1981 |
| Externally published | Yes |
Fingerprint
Keywords
- astrocytes
- basic protein
- galactocerebroside
- glial fibrillary acidic protein
- long-term culture
- oligodendroglia
- Percoll
- phenotypic markers
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Long term culture of bovine oligodendroglia isolated with a percoll gradient. / Lisak, Robert P.; Pleasure, David E; Silberberg, Donald H.; Manning, Margaret C.; Saida, Takahiko.
In: Brain Research, Vol. 223, No. 1, 26.10.1981, p. 107-122.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Long term culture of bovine oligodendroglia isolated with a percoll gradient
AU - Lisak, Robert P.
AU - Pleasure, David E
AU - Silberberg, Donald H.
AU - Manning, Margaret C.
AU - Saida, Takahiko
PY - 1981/10/26
Y1 - 1981/10/26
N2 - Oligodendroglia were isolated from calf central nervous system (CNS) white matter by trypsinization in phosphate buffered saline and separation by centrifugation through Percoll. Using antisera to phenotypic markers and double labelling experiments we were able to identify essentially all cells in the cultures. The cells obtained were: (1) viable; (2) had intact plasma membranes and well preserved organelles, ribosomes and mitochondria; and (3) were ≥95% oligodendroglia 16-20 h after isolation as determined by ability to bind antigalactocerebroside antibodies (anti-GalC). Oligodendroglia could be cultured for several weeks to months. Oligodendroglia established and maintained processes which bound anti-GalC. Myelin basic protein could be demonstrated in the cytoplasm of 40-60% of oligodendroglia cell bodies but not in the processes.
AB - Oligodendroglia were isolated from calf central nervous system (CNS) white matter by trypsinization in phosphate buffered saline and separation by centrifugation through Percoll. Using antisera to phenotypic markers and double labelling experiments we were able to identify essentially all cells in the cultures. The cells obtained were: (1) viable; (2) had intact plasma membranes and well preserved organelles, ribosomes and mitochondria; and (3) were ≥95% oligodendroglia 16-20 h after isolation as determined by ability to bind antigalactocerebroside antibodies (anti-GalC). Oligodendroglia could be cultured for several weeks to months. Oligodendroglia established and maintained processes which bound anti-GalC. Myelin basic protein could be demonstrated in the cytoplasm of 40-60% of oligodendroglia cell bodies but not in the processes.
KW - astrocytes
KW - basic protein
KW - galactocerebroside
KW - glial fibrillary acidic protein
KW - long-term culture
KW - oligodendroglia
KW - Percoll
KW - phenotypic markers
UR - http://www.scopus.com/inward/record.url?scp=0019407735&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019407735&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(81)90809-X
DO - 10.1016/0006-8993(81)90809-X
M3 - Article
C2 - 6269698
AN - SCOPUS:0019407735
VL - 223
SP - 107
EP - 122
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -