Long term culture of bovine oligodendroglia isolated with a percoll gradient

Robert P. Lisak, David E Pleasure, Donald H. Silberberg, Margaret C. Manning, Takahiko Saida

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Oligodendroglia were isolated from calf central nervous system (CNS) white matter by trypsinization in phosphate buffered saline and separation by centrifugation through Percoll. Using antisera to phenotypic markers and double labelling experiments we were able to identify essentially all cells in the cultures. The cells obtained were: (1) viable; (2) had intact plasma membranes and well preserved organelles, ribosomes and mitochondria; and (3) were ≥95% oligodendroglia 16-20 h after isolation as determined by ability to bind antigalactocerebroside antibodies (anti-GalC). Oligodendroglia could be cultured for several weeks to months. Oligodendroglia established and maintained processes which bound anti-GalC. Myelin basic protein could be demonstrated in the cytoplasm of 40-60% of oligodendroglia cell bodies but not in the processes.

Original languageEnglish (US)
Pages (from-to)107-122
Number of pages16
JournalBrain Research
Volume223
Issue number1
DOIs
StatePublished - Oct 26 1981
Externally publishedYes

Fingerprint

Oligodendroglia
Myelin Basic Protein
Antibodies
Centrifugation
Ribosomes
Organelles
Immune Sera
Mitochondria
Cytoplasm
Central Nervous System
Cell Culture Techniques
Phosphates
Cell Membrane
Percoll

Keywords

  • astrocytes
  • basic protein
  • galactocerebroside
  • glial fibrillary acidic protein
  • long-term culture
  • oligodendroglia
  • Percoll
  • phenotypic markers

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Long term culture of bovine oligodendroglia isolated with a percoll gradient. / Lisak, Robert P.; Pleasure, David E; Silberberg, Donald H.; Manning, Margaret C.; Saida, Takahiko.

In: Brain Research, Vol. 223, No. 1, 26.10.1981, p. 107-122.

Research output: Contribution to journalArticle

Lisak, Robert P. ; Pleasure, David E ; Silberberg, Donald H. ; Manning, Margaret C. ; Saida, Takahiko. / Long term culture of bovine oligodendroglia isolated with a percoll gradient. In: Brain Research. 1981 ; Vol. 223, No. 1. pp. 107-122.
@article{5dfd885063ae426da2c1386ba6de88a8,
title = "Long term culture of bovine oligodendroglia isolated with a percoll gradient",
abstract = "Oligodendroglia were isolated from calf central nervous system (CNS) white matter by trypsinization in phosphate buffered saline and separation by centrifugation through Percoll. Using antisera to phenotypic markers and double labelling experiments we were able to identify essentially all cells in the cultures. The cells obtained were: (1) viable; (2) had intact plasma membranes and well preserved organelles, ribosomes and mitochondria; and (3) were ≥95{\%} oligodendroglia 16-20 h after isolation as determined by ability to bind antigalactocerebroside antibodies (anti-GalC). Oligodendroglia could be cultured for several weeks to months. Oligodendroglia established and maintained processes which bound anti-GalC. Myelin basic protein could be demonstrated in the cytoplasm of 40-60{\%} of oligodendroglia cell bodies but not in the processes.",
keywords = "astrocytes, basic protein, galactocerebroside, glial fibrillary acidic protein, long-term culture, oligodendroglia, Percoll, phenotypic markers",
author = "Lisak, {Robert P.} and Pleasure, {David E} and Silberberg, {Donald H.} and Manning, {Margaret C.} and Takahiko Saida",
year = "1981",
month = "10",
day = "26",
doi = "10.1016/0006-8993(81)90809-X",
language = "English (US)",
volume = "223",
pages = "107--122",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Long term culture of bovine oligodendroglia isolated with a percoll gradient

AU - Lisak, Robert P.

AU - Pleasure, David E

AU - Silberberg, Donald H.

AU - Manning, Margaret C.

AU - Saida, Takahiko

PY - 1981/10/26

Y1 - 1981/10/26

N2 - Oligodendroglia were isolated from calf central nervous system (CNS) white matter by trypsinization in phosphate buffered saline and separation by centrifugation through Percoll. Using antisera to phenotypic markers and double labelling experiments we were able to identify essentially all cells in the cultures. The cells obtained were: (1) viable; (2) had intact plasma membranes and well preserved organelles, ribosomes and mitochondria; and (3) were ≥95% oligodendroglia 16-20 h after isolation as determined by ability to bind antigalactocerebroside antibodies (anti-GalC). Oligodendroglia could be cultured for several weeks to months. Oligodendroglia established and maintained processes which bound anti-GalC. Myelin basic protein could be demonstrated in the cytoplasm of 40-60% of oligodendroglia cell bodies but not in the processes.

AB - Oligodendroglia were isolated from calf central nervous system (CNS) white matter by trypsinization in phosphate buffered saline and separation by centrifugation through Percoll. Using antisera to phenotypic markers and double labelling experiments we were able to identify essentially all cells in the cultures. The cells obtained were: (1) viable; (2) had intact plasma membranes and well preserved organelles, ribosomes and mitochondria; and (3) were ≥95% oligodendroglia 16-20 h after isolation as determined by ability to bind antigalactocerebroside antibodies (anti-GalC). Oligodendroglia could be cultured for several weeks to months. Oligodendroglia established and maintained processes which bound anti-GalC. Myelin basic protein could be demonstrated in the cytoplasm of 40-60% of oligodendroglia cell bodies but not in the processes.

KW - astrocytes

KW - basic protein

KW - galactocerebroside

KW - glial fibrillary acidic protein

KW - long-term culture

KW - oligodendroglia

KW - Percoll

KW - phenotypic markers

UR - http://www.scopus.com/inward/record.url?scp=0019407735&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019407735&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(81)90809-X

DO - 10.1016/0006-8993(81)90809-X

M3 - Article

C2 - 6269698

AN - SCOPUS:0019407735

VL - 223

SP - 107

EP - 122

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -