Long term culture of bovine oligodendroglia isolated with a percoll gradient

Robert P. Lisak, David E Pleasure, Donald H. Silberberg, Margaret C. Manning, Takahiko Saida

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Oligodendroglia were isolated from calf central nervous system (CNS) white matter by trypsinization in phosphate buffered saline and separation by centrifugation through Percoll. Using antisera to phenotypic markers and double labelling experiments we were able to identify essentially all cells in the cultures. The cells obtained were: (1) viable; (2) had intact plasma membranes and well preserved organelles, ribosomes and mitochondria; and (3) were ≥95% oligodendroglia 16-20 h after isolation as determined by ability to bind antigalactocerebroside antibodies (anti-GalC). Oligodendroglia could be cultured for several weeks to months. Oligodendroglia established and maintained processes which bound anti-GalC. Myelin basic protein could be demonstrated in the cytoplasm of 40-60% of oligodendroglia cell bodies but not in the processes.

Original languageEnglish (US)
Pages (from-to)107-122
Number of pages16
JournalBrain Research
Volume223
Issue number1
DOIs
StatePublished - Oct 26 1981
Externally publishedYes

Keywords

  • astrocytes
  • basic protein
  • galactocerebroside
  • glial fibrillary acidic protein
  • long-term culture
  • oligodendroglia
  • Percoll
  • phenotypic markers

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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