Long-term calorie restriction reduces proton leak and hydrogen peroxide production in liver mitochondria

Kevork Hagopian, Mary Ellen Harper, Jesmon J. Ram, Stephen J. Humble, Richard Weindruch, Jon J Ramsey

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Calorie restriction (CR) without malnutrition increases maximal life span in diverse species. It has been proposed that reduction in energy expenditure and reactive oxygen species (ROS) production could be a mechanism for life span extension with CR. As a step toward testing this theory, mitochondrial proton leak, H2O2 production, and markers of oxidative stress were measured in liver from FBNF1 rats fed control or 40% CR diets for 12 or 18 mo. CR was initiated at 6 mo of age. Proton leak kinetics curves, generated from simultaneous measures of oxygen consumption and membrane potential, indicated a decrease in proton leak after 18 mo of CR, while only a trend toward a proton leak decrease was observed after 12 mo. Significant shifts in phosphorylation and substrate oxidation curves also occurred with CR; however, these changes occurred in concert with the proton leak changes. Metabolic control analysis indicated no difference in the overall pattern of control of the oxidative phosphorylation system between control and CR animals. At 12 mo, no significant differences were observed between groups for H 2O2 production or markers of oxidative stress. However, at 18 mo, protein carbonyl content was lower in CR animals, as was H 2O2 production when mitochondria were respiring on either succinate alone or pyruvate plus malate in the presence of rotenone. These results indicate that long-term CR lowers mitochondrial proton leak and H 2O2 production, and this is consistent with the idea that CR may act by decreasing energy expenditure and ROS production.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume288
Issue number4 51-4
DOIs
StatePublished - Apr 2005

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Mitochondria
Liver Mitochondrion
Liver
Hydrogen Peroxide
Protons
Oxidative stress
Energy Metabolism
Reactive Oxygen Species
Animals
Oxidative Stress
Rotenone
Phosphorylation
Oxidative Phosphorylation
Succinic Acid
Nutrition
Life Expectancy
Pyruvic Acid
Oxygen Consumption
Malnutrition
Membrane Potentials

Keywords

  • Aging
  • Energy expenditure
  • Food restriction
  • Oxidative stress

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Long-term calorie restriction reduces proton leak and hydrogen peroxide production in liver mitochondria. / Hagopian, Kevork; Harper, Mary Ellen; Ram, Jesmon J.; Humble, Stephen J.; Weindruch, Richard; Ramsey, Jon J.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 288, No. 4 51-4, 04.2005.

Research output: Contribution to journalArticle

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