Long experimental durations are required for double label [14C]- and [3H]2-deoxyglucose autoradiographic methods

Samuel F. Ciricillo, Mary P. Jasper, Manuel F. Gonzalez, Frank R Sharp

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Double-label 2-deoxyglucose (2-DG) studies using sequential [14C]- and [3H]2-DG injections demonstrate increased [14C]2-DG uptake durating the first and second stimulation periods. To understand why this occurs, the rat mystacial vibrissae were stimulated at various times following [14C]2-DG injection. Local cerebral glucose utilization (LCGU) increased when whisker stimulation was performed at 0-90 min following [14C]2-DG injection. LCGU did not increase when whisker stimulation was performed at 90-150 min following [14C]2-DG injection. To minimize contamination of the two tracers in double label 2-DG mapping studies, the time between [14C]- and [3H]2-DG administration should be increased to 90 min.

Original languageEnglish (US)
Pages (from-to)171-175
Number of pages5
JournalBrain Research
Volume564
Issue number1
DOIs
StatePublished - Nov 8 1991
Externally publishedYes

Keywords

  • 2-Deoxyglucose
  • Autoradiography
  • Brain glucose utilization
  • Double-label
  • Rat vibrissa

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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