Long-chain fatty acid combustion rate is associated with unique metabolite profiles in skeletal muscle mitochondria

Erin L. Seifert, Oliver Fiehn, Véronic Bezaire, David R. Bickel, Gert Wohlgemuth, Sean H. Adams, Mary Ellen Harper

Research output: Contribution to journalArticle

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Abstract

Background/Aim: Incomplete or limited long-chain fatty acid (LCFA) combustion in skeletal muscle has been associated with insulin resistance. Signals that are responsive to shifts in LCFA ß-oxidation rate or degree of intramitochondrial catabolism are hypothesized to regulate second messenger systems downstream of the insulin receptor. Recent evidence supports a causal link between mitochondrial LCFA combustion in skeletal muscle and insulin resistance. We have used unbiased metabolite profiling of mouse muscle mitochondria with the aim of identifying candidate metabolites within or effluxed from mitochondria and that are shifted with LCFA combustion rate. Methodology/Principal Findings: Large-scale unbiased metabolomics analysis was performed using GC/TOF-MS on buffer and mitochondrial matrix fractions obtained prior to and after 20 min of palmitate catabolism (n = 7 mice/condition). Three palmitate concentrations (2, 9 and 19 mM; corresponding to low, intermediate and high oxidation rates) and 9 μM palmitate plus tricarboxylic acid (TCA) cycle and electron transport chain inhibitors were each tested and compared to zero palmitate control incubations. Paired comparisons of the 0 and 20 min samples were made by Student's t-test. False discovery rate were estimated and Type I error rates assigned. Major metabolite groups were organic acids, amines and amino acids, free fatty acids and sugar phosphates. Palmitate oxidation was associated with unique profiles of metabolites, a subset of which correlated to palmitate oxidation rate. In particular, palmitate oxidation rate was associated with distinct changes in the levels of TCA cycle intermediates within and effluxed from mitochondria. Conclusions/Significance: This proof-of-principle study establishes that large-scale metabolomics methods can be applied to organelle-level models to discover metabolite patterns reflective of LCFA combustion, which may lead to identification of molecules linking muscle fat metabolism and insulin signaling. Our results suggest that future studies should focus on the fate of effluxed TCA cycle intermediates and on mechanisms ensuring their replenishment during LCFA metabolism in skeletal muscle.

Original languageEnglish (US)
Article numbere9834
JournalPLoS One
Volume5
Issue number3
DOIs
StatePublished - 2010

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Muscle Mitochondrion
Mitochondria
Palmitates
palmitates
long chain fatty acids
Metabolites
combustion
Muscle
skeletal muscle
Skeletal Muscle
mitochondria
Fatty Acids
metabolites
Citric Acid Cycle
tricarboxylic acid cycle
Oxidation
oxidation
Metabolomics
metabolomics
Insulin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Seifert, E. L., Fiehn, O., Bezaire, V., Bickel, D. R., Wohlgemuth, G., Adams, S. H., & Harper, M. E. (2010). Long-chain fatty acid combustion rate is associated with unique metabolite profiles in skeletal muscle mitochondria. PLoS One, 5(3), [e9834]. https://doi.org/10.1371/journal.pone.0009834

Long-chain fatty acid combustion rate is associated with unique metabolite profiles in skeletal muscle mitochondria. / Seifert, Erin L.; Fiehn, Oliver; Bezaire, Véronic; Bickel, David R.; Wohlgemuth, Gert; Adams, Sean H.; Harper, Mary Ellen.

In: PLoS One, Vol. 5, No. 3, e9834, 2010.

Research output: Contribution to journalArticle

Seifert, EL, Fiehn, O, Bezaire, V, Bickel, DR, Wohlgemuth, G, Adams, SH & Harper, ME 2010, 'Long-chain fatty acid combustion rate is associated with unique metabolite profiles in skeletal muscle mitochondria', PLoS One, vol. 5, no. 3, e9834. https://doi.org/10.1371/journal.pone.0009834
Seifert, Erin L. ; Fiehn, Oliver ; Bezaire, Véronic ; Bickel, David R. ; Wohlgemuth, Gert ; Adams, Sean H. ; Harper, Mary Ellen. / Long-chain fatty acid combustion rate is associated with unique metabolite profiles in skeletal muscle mitochondria. In: PLoS One. 2010 ; Vol. 5, No. 3.
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