Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell-cell adhesion

Soichiro Yamada, W. James Nelson

Research output: Contribution to journalArticle

318 Citations (Scopus)

Abstract

Spatiotemporal coordination of cell-cell adhesion involving lamellipodial interactions, cadherin engagement, and the lateral expansion of the contact is poorly understood. Using high-resolution live-cell imaging, biosensors, and small molecule inhibitors, we investigate how Rac1 and RhoA regulate actin dynamics during de novo contact formation between pairs of epithelial cells. Active Rac1, the Arp2/3 complex, and lamellipodia are initially localized to de novo contacts but rapidly diminish as E-cadherin accumulates; further rounds of activation and down-regulation of Rac1 and Arp2/3 occur at the contacting membrane periphery, and this cycle repeats as a restricted membrane zone that moves outward with the expanding contact. The cortical bundle of actin filaments dissolves beneath the expanding contacts, leaving actin bundles at the contact edges. RhoA and actomyosin contractility are activated at the contact edges and are required to drive expansion and completion of cell-cell adhesion. We show that zones of Rac1 and lamellipodia activity and of RhoA and actomyosin contractility are restricted to the periphery of contacting membranes and together drive initiation, expansion, and completion of cell-cell adhesion.

Original languageEnglish (US)
Pages (from-to)517-527
Number of pages11
JournalJournal of Cell Biology
Volume178
Issue number3
DOIs
StatePublished - Jul 30 2007

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Cell Adhesion
Epithelial Cells
Actomyosin
Pseudopodia
Cadherins
Membranes
Actins
Actin-Related Protein 2-3 Complex
Biosensing Techniques
Actin Cytoskeleton
Down-Regulation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell-cell adhesion. / Yamada, Soichiro; Nelson, W. James.

In: Journal of Cell Biology, Vol. 178, No. 3, 30.07.2007, p. 517-527.

Research output: Contribution to journalArticle

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