Localized Release of Serotonin (5-Hydroxytryptamine) by a Fecal Pellet Regulates Migrating Motor Complexes in Murine Colon

Dante J. Heredia, Eamonn J Dickson, Peter O. Bayguinov, Grant W. Hennig, Terence K. Smith

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Background & Aims: The colonic migrating motor complex (CMMC) is a motor pattern that regulates the movement of fecal matter through a rhythmic sequence of electrical activity and/or contractions along the large bowel. CMMCs have largely been studied in empty preparations; we investigated whether local reflexes generated by a fecal pellet modify the CMMC to initiate propulsive activity. Methods: Recordings of CMMCs were made from the isolated murine large bowel, with or without a fecal pellet. Transducers were placed along the colon to record muscle tension and propulsive force on the pellet and microelectrodes were used to record electrical activity from either side of a fecal pellet, circular muscle cells oral and anal of a pellet, and in colons without the mucosa. Results: Spontaneous CMMCs propagated in both an oral or anal direction. When a pellet was inserted, CMMCs increased in frequency and propagated anally, exerting propulsive force on the pellet. The amplitude of slow waves increased during the CMMC. Localized mucosal stimulation/circumferential stretch evoked a CMMC, regardless of stimulus strength. The serotonin (5-hydroxytryptamine-3) receptor antagonist ondansetron reduced the amplitude of the CMMC, the propulsive force on the pellet, and the response to mucosal stroking, but increased the apparent conduction velocity of the CMMC. Removing the mucosa abolished spontaneous CMMCs, which still could be evoked by electrical stimulation. Conclusions: The fecal pellet activates local mucosal reflexes, which release serotonin (5-hydroxytryptamine) from enterochromaffin cells, and stretch reflexes that determine the site of origin and propagation of the CMMC, facilitating propulsion.

Original languageEnglish (US)
Pages (from-to)1328-1338
Number of pages11
JournalGastroenterology
Volume136
Issue number4
DOIs
StatePublished - Apr 2009
Externally publishedYes

Fingerprint

Migrating Myoelectric Complexes
Serotonin
Colon
Reflex
Mucous Membrane
Enterochromaffin Cells
Receptors, Serotonin, 5-HT3
Ondansetron
Stretch Reflex
Muscle Tonus
Microelectrodes
Transducers
Muscle Cells
Electric Stimulation
6-cyano-5-methoxy-12-methylindolo(2,3-a)carbazole

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Localized Release of Serotonin (5-Hydroxytryptamine) by a Fecal Pellet Regulates Migrating Motor Complexes in Murine Colon. / Heredia, Dante J.; Dickson, Eamonn J; Bayguinov, Peter O.; Hennig, Grant W.; Smith, Terence K.

In: Gastroenterology, Vol. 136, No. 4, 04.2009, p. 1328-1338.

Research output: Contribution to journalArticle

Heredia, Dante J. ; Dickson, Eamonn J ; Bayguinov, Peter O. ; Hennig, Grant W. ; Smith, Terence K. / Localized Release of Serotonin (5-Hydroxytryptamine) by a Fecal Pellet Regulates Migrating Motor Complexes in Murine Colon. In: Gastroenterology. 2009 ; Vol. 136, No. 4. pp. 1328-1338.
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