Localized populations of CD8low/- MHC class I tetramer+ SIV-specific T cells in lymphoid follicles and genital epithelium

Jung Joo Hong, Matthew R. Reynolds, Teresa L. Mattila, Aaron Hage, David I. Watkins, Chris J Miller, Pamela J. Skinner

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

CD8 T cells play an important role in controlling viral infections. We investigated the in situ localization of simian immunodeficiency virus (SIV)-specific T cells in lymph and genital tissues from SIV-infected macaques using MHC-class I tetramers. The majority of tetramer-binding cells localized in T cell zones and were CD8+. Curiously, small subpopulations of tetramer-binding cells that had little to no surface CD8 were detected in situ both early and late post-infection, and in both vaginally and rectally inoculated macaques. These tetramer+CD8low/- cells were more often localized in apparent B cell follicles relative to T cell zones and more often found near or within the genital epithelium than the submucosa. Cells analyzed by flow cytometry showed similar populations of cells. Further immunohistological characterization revealed small populations of tetramer+CD20- cells inside B cell follicles and that tetramer+ cells did not stain with γδ-TCR nor CD4 antibodies. Negative control tetramer staining indicated that tetramer+CD8low/- cells were not likely NK cells non-specifically binding to MHC tetramers. These findings have important implications for SIV-specific and other antigen-specific T cell function in these specific tissue locations, and suggest a model in which antigen-specific CD8+ T cells down modulate CD8 upon entering B cell follicles or the epithelial layer of tissues, or alternatively a model in which only antigen-specific CD8 T cells that down-modulate CD8 can enter B cell follicles or the epithelium.

Original languageEnglish (US)
Article numbere4131
JournalPLoS One
Volume4
Issue number1
DOIs
StatePublished - Jan 5 2009

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Simian immunodeficiency virus
Simian Immunodeficiency Virus
T-cells
Viruses
genitalia
epithelium
Epithelium
T-lymphocytes
T-Lymphocytes
Cells
Population
B-lymphocytes
B-Lymphocytes
cells
CD8 Antigens
Tissue
Antigens
Macaca
antigens
Flow cytometry

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Localized populations of CD8low/- MHC class I tetramer+ SIV-specific T cells in lymphoid follicles and genital epithelium. / Hong, Jung Joo; Reynolds, Matthew R.; Mattila, Teresa L.; Hage, Aaron; Watkins, David I.; Miller, Chris J; Skinner, Pamela J.

In: PLoS One, Vol. 4, No. 1, e4131, 05.01.2009.

Research output: Contribution to journalArticle

Hong, Jung Joo ; Reynolds, Matthew R. ; Mattila, Teresa L. ; Hage, Aaron ; Watkins, David I. ; Miller, Chris J ; Skinner, Pamela J. / Localized populations of CD8low/- MHC class I tetramer+ SIV-specific T cells in lymphoid follicles and genital epithelium. In: PLoS One. 2009 ; Vol. 4, No. 1.
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