The chicken phosphoglycerate kinase (PgK) gene has been mapped to the short arm of chromosome 4 by fluorescence in situ hybridization. Biotinylated probes of cloned chicken PgK DNA were hybridized to metaphase chromosomes prepared from MSB-1 lymphocytes and chick embryo fibroblasts. This technique used one layer of signal amplification, which allowed DNA sequences of 2 Kb to be detected. Mapping PgK to an autosome in chickens provides further evidence that the avian Z chromosome and the mammalian X chromosome are not homologous.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Heredity|
|State||Published - 1994|
ASJC Scopus subject areas
- Molecular Biology
- Agricultural and Biological Sciences(all)