Localization of the Chicken Pgk Gene to Chromosome 4p by Fluorescence in situ Hybridization

Katherine A Rauen, C. D S Le Ciel, U. K. Abbott, N. J. Hutchison

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The chicken phosphoglycerate kinase (PgK) gene has been mapped to the short arm of chromosome 4 by fluorescence in situ hybridization. Biotinylated probes of cloned chicken PgK DNA were hybridized to metaphase chromosomes prepared from MSB-1 lymphocytes and chick embryo fibroblasts. This technique used one layer of signal amplification, which allowed DNA sequences of 2 Kb to be detected. Mapping PgK to an autosome in chickens provides further evidence that the avian Z chromosome and the mammalian X chromosome are not homologous.

Original languageEnglish (US)
Pages (from-to)147-150
Number of pages4
JournalJournal of Heredity
Issue number2
StatePublished - 1994


ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Biotechnology
  • Molecular Biology
  • Agricultural and Biological Sciences(all)

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