Localization of the Chicken Pgk Gene to Chromosome 4p by Fluorescence in situ Hybridization

Katherine A Rauen, C. D S Le Ciel, U. K. Abbott, N. J. Hutchison

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The chicken phosphoglycerate kinase (PgK) gene has been mapped to the short arm of chromosome 4 by fluorescence in situ hybridization. Biotinylated probes of cloned chicken PgK DNA were hybridized to metaphase chromosomes prepared from MSB-1 lymphocytes and chick embryo fibroblasts. This technique used one layer of signal amplification, which allowed DNA sequences of 2 Kb to be detected. Mapping PgK to an autosome in chickens provides further evidence that the avian Z chromosome and the mammalian X chromosome are not homologous.

Original languageEnglish (US)
Pages (from-to)147-150
Number of pages4
JournalJournal of Heredity
Volume85
Issue number2
DOIs
StatePublished - 1994

Fingerprint

phosphoglycerate kinase
Phosphoglycerate Kinase
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
Chickens
Chromosomes
chickens
chromosomes
Mammalian Chromosomes
Genes
Z chromosome
Chromosomes, Human, Pair 4
genes
autosomes
X Chromosome
X chromosome
Chick Embryo
Metaphase
metaphase
fibroblasts

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Biotechnology
  • Molecular Biology
  • Agricultural and Biological Sciences(all)

Cite this

Localization of the Chicken Pgk Gene to Chromosome 4p by Fluorescence in situ Hybridization. / Rauen, Katherine A; Le Ciel, C. D S; Abbott, U. K.; Hutchison, N. J.

In: Journal of Heredity, Vol. 85, No. 2, 1994, p. 147-150.

Research output: Contribution to journalArticle

Rauen, Katherine A ; Le Ciel, C. D S ; Abbott, U. K. ; Hutchison, N. J. / Localization of the Chicken Pgk Gene to Chromosome 4p by Fluorescence in situ Hybridization. In: Journal of Heredity. 1994 ; Vol. 85, No. 2. pp. 147-150.
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