Localization of Osteogenic Protein-1 (Bone Morphogenetic Protein-7) during Human Embryonic Development: High Affinity Binding to Basement Membranes

S. Vukicevic, V. Latin, P. Chen, R. Batorsky, A Hari Reddi, T. K. Sampath

Research output: Contribution to journalArticlepeer-review

177 Scopus citations

Abstract

Osteogenic protein-1 (OP-1) is a member of the bone morphogenetic protein subfamily of the transforming growth factor-beta (TGF-β) superfamily. Since members of the TGF-β superfamily have a role in tissue development the distribution of OP-1 in developing human embryos (gestational age 5-14 weeks) was examined by immunohistochemical methods. Positive staining for OP-1 was observed in: sclerotome, hypertrophied chondrocytes, osteoblasts, periosteum, epithelial cells of the adrenal "provisional cortex" and the convoluted tubules of developing kidneys. In the developing lungs, pancreas and skin, OP-1 was localized in basement membranes underlying the epitheliurn. In vitro binding studies of 125I-OP-1 to various extracellular matrix components revealed high affinity of OP-1 for type IV collagen and less for heparin, collagen types I and VI. Present findings suggest that, in addition to bone formation, OP-1 could have other important regulatory roles in human embryogenesis with high binding affinity to a basement membrane component.

Original languageEnglish (US)
Pages (from-to)693-700
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume198
Issue number2
DOIs
StatePublished - Jan 31 1994
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

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