Abstract
Binding of [3H]ouabain by the dog's tracheal epithelium shows a nonspecific component depending linearly on ouabain concentration, and a specific saturable component with a K(m) of 10-7 M. Control experiments showed that the tracer taken up was not trapped within the extracellular space nor bound to tissue collagen. Inhibition of the saturable uptake by high K, metabolic inhibition, low Na, and low temperature indicated that binding was to Na/K ATPase. One-sided exposure of tissue sheets to tracer showed that the submucosal side took up 10 x as much tracer as the luminal. Autoradiography localized tracer uptake under all conditions to the cells' basolateral membranes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 380-390 |
| Number of pages | 11 |
| Journal | Journal of Cell Biology |
| Volume | 82 |
| Issue number | 2 |
| State | Published - 1979 |
| Externally published | Yes |
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Cite this
Localization of Na pumps in the tracheal epithelium of the dog. / Widdicombe, Jonathan; Basbaum, C. B.; Yee, J. Y.
In: Journal of Cell Biology, Vol. 82, No. 2, 1979, p. 380-390.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Localization of Na pumps in the tracheal epithelium of the dog
AU - Widdicombe, Jonathan
AU - Basbaum, C. B.
AU - Yee, J. Y.
PY - 1979
Y1 - 1979
N2 - Binding of [3H]ouabain by the dog's tracheal epithelium shows a nonspecific component depending linearly on ouabain concentration, and a specific saturable component with a K(m) of 10-7 M. Control experiments showed that the tracer taken up was not trapped within the extracellular space nor bound to tissue collagen. Inhibition of the saturable uptake by high K, metabolic inhibition, low Na, and low temperature indicated that binding was to Na/K ATPase. One-sided exposure of tissue sheets to tracer showed that the submucosal side took up 10 x as much tracer as the luminal. Autoradiography localized tracer uptake under all conditions to the cells' basolateral membranes.
AB - Binding of [3H]ouabain by the dog's tracheal epithelium shows a nonspecific component depending linearly on ouabain concentration, and a specific saturable component with a K(m) of 10-7 M. Control experiments showed that the tracer taken up was not trapped within the extracellular space nor bound to tissue collagen. Inhibition of the saturable uptake by high K, metabolic inhibition, low Na, and low temperature indicated that binding was to Na/K ATPase. One-sided exposure of tissue sheets to tracer showed that the submucosal side took up 10 x as much tracer as the luminal. Autoradiography localized tracer uptake under all conditions to the cells' basolateral membranes.
UR - http://www.scopus.com/inward/record.url?scp=0018634521&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018634521&partnerID=8YFLogxK
M3 - Article
C2 - 225337
AN - SCOPUS:0018634521
VL - 82
SP - 380
EP - 390
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 2
ER -