Localization of Na pumps in the tracheal epithelium of the dog

Jonathan Widdicombe, C. B. Basbaum, J. Y. Yee

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Binding of [3H]ouabain by the dog's tracheal epithelium shows a nonspecific component depending linearly on ouabain concentration, and a specific saturable component with a K(m) of 10-7 M. Control experiments showed that the tracer taken up was not trapped within the extracellular space nor bound to tissue collagen. Inhibition of the saturable uptake by high K, metabolic inhibition, low Na, and low temperature indicated that binding was to Na/K ATPase. One-sided exposure of tissue sheets to tracer showed that the submucosal side took up 10 x as much tracer as the luminal. Autoradiography localized tracer uptake under all conditions to the cells' basolateral membranes.

Original languageEnglish (US)
Pages (from-to)380-390
Number of pages11
JournalJournal of Cell Biology
Volume82
Issue number2
StatePublished - 1979
Externally publishedYes

Fingerprint

Ouabain
Epithelium
Dogs
Extracellular Space
Autoradiography
Collagen
Cell Membrane
Temperature
sodium-translocating ATPase

ASJC Scopus subject areas

  • Cell Biology

Cite this

Localization of Na pumps in the tracheal epithelium of the dog. / Widdicombe, Jonathan; Basbaum, C. B.; Yee, J. Y.

In: Journal of Cell Biology, Vol. 82, No. 2, 1979, p. 380-390.

Research output: Contribution to journalArticle

Widdicombe, Jonathan ; Basbaum, C. B. ; Yee, J. Y. / Localization of Na pumps in the tracheal epithelium of the dog. In: Journal of Cell Biology. 1979 ; Vol. 82, No. 2. pp. 380-390.
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