Localization of glucocorticoid receptors at postsynaptic membranes in the lateral amygdala

L. R. Johnson, C. Farb, John Morrison, B. S. McEwen, J. E. LeDoux

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Glucocorticoids, released in high concentrations from the adrenal cortex during stressful experiences, bind to glucocorticoid receptors in nuclear and peri-nuclear sites in neuronal somata. Their classically known mode of action is to induce gene promoter receptors to alter gene transcription. Nuclear glucocorticoid receptors are particularly dense in brain regions crucial for memory, including memory of stressful experiences, such as the hippocampus and amygdala. While it has been proposed that glucocorticoids may also act via membrane bound receptors, the existence of the latter remains controversial. Using electron microscopy, we found glucocorticoid receptors localized to non-genomic sites in rat lateral amygdala, glia processes, presynaptic terminals, neuronal dendrites, and dendritic spines including spine organelles and postsynaptic membrane densities. The lateral nucleus of the amygdala is a region specifically implicated in the formation of memories for stressful experiences. These newly observed glucocorticoid receptor immunoreactive sites were in addition to glucocorticoid receptor immunoreactive signals observed using electron and confocal microscopy in lateral amygdala principal neuron and GABA neuron soma and nuclei, cellular domains traditionally associated with glucocorticoid immunoreactivity. In lateral amygdala, glucocorticoid receptors are thus also localized to non-nuclear-membrane translocation sites, particularly dendritic spines, where they show an affinity for postsynaptic membrane densities, and may have a specialized role in modulating synaptic transmission plasticity related to fear and emotional memory.

Original languageEnglish (US)
Pages (from-to)289-299
Number of pages11
JournalNeuroscience
Volume136
Issue number1
DOIs
StatePublished - Oct 31 2005
Externally publishedYes

Fingerprint

Glucocorticoid Receptors
Amygdala
Membranes
Glucocorticoids
Post-Synaptic Density
Dendritic Spines
Carisoprodol
Electron Microscopy
GABAergic Neurons
Neuronal Plasticity
Adrenal Cortex
Presynaptic Terminals
Cytoplasmic and Nuclear Receptors
Dendrites
Confocal Microscopy
Synaptic Transmission
Neuroglia
Organelles
Genes
Fear

Keywords

  • Fear
  • Non-genomic
  • Plasticity
  • Postsynaptic density

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Localization of glucocorticoid receptors at postsynaptic membranes in the lateral amygdala. / Johnson, L. R.; Farb, C.; Morrison, John; McEwen, B. S.; LeDoux, J. E.

In: Neuroscience, Vol. 136, No. 1, 31.10.2005, p. 289-299.

Research output: Contribution to journalArticle

Johnson, L. R. ; Farb, C. ; Morrison, John ; McEwen, B. S. ; LeDoux, J. E. / Localization of glucocorticoid receptors at postsynaptic membranes in the lateral amygdala. In: Neuroscience. 2005 ; Vol. 136, No. 1. pp. 289-299.
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