Localization and targeting of voltage-dependent ion channels in mammalian central neurons

Helene Vacher, Durga P. Mohapatra, James Trimmer

Research output: Contribution to journalArticle

302 Citations (Scopus)

Abstract

The intrinsic electrical properties and the synaptic input-output relationships of neurons are governed by the action of voltagedependent ion channels. The localization of specific populations of ion channels with distinct functional properties at discrete sites in neurons dramatically impacts excitability and synaptic transmission. Molecular cloning studies have revealed a large family of genes encoding voltage-dependent ion channel principal and auxiliary subunits, most of which are expressed in mammalian central neurons. Much recent effort has focused on determining which of these subunits coassemble into native neuronal channel complexes, and the cellular and subcellular distributions of these complexes, as a crucial step in understanding the contribution of these channels to specific aspects of neuronal function. Here we review progress made on recent studies aimed to determine the cellular and subcellular distribution of specific ion channel subunits in mammalian brain neurons using in situ hybridization and immunohistochemistry. We also discuss the repertoire of ion channel subunits in specific neuronal compartments and implications for neuronal physiology. Finally, we discuss the emerging mechanisms for determining the discrete subcellular distributions observed for many neuronal ion channels.

Original languageEnglish (US)
Pages (from-to)1407-1447
Number of pages41
JournalPhysiological Reviews
Volume88
Issue number4
DOIs
StatePublished - Oct 2008

Fingerprint

Ion Channels
Neurons
Molecular Cloning
Synaptic Transmission
In Situ Hybridization
Immunohistochemistry
Brain
Population
Genes

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Physiology (medical)

Cite this

Localization and targeting of voltage-dependent ion channels in mammalian central neurons. / Vacher, Helene; Mohapatra, Durga P.; Trimmer, James.

In: Physiological Reviews, Vol. 88, No. 4, 10.2008, p. 1407-1447.

Research output: Contribution to journalArticle

Vacher, Helene ; Mohapatra, Durga P. ; Trimmer, James. / Localization and targeting of voltage-dependent ion channels in mammalian central neurons. In: Physiological Reviews. 2008 ; Vol. 88, No. 4. pp. 1407-1447.
@article{d319ac60753c4abc85af6bee7f866541,
title = "Localization and targeting of voltage-dependent ion channels in mammalian central neurons",
abstract = "The intrinsic electrical properties and the synaptic input-output relationships of neurons are governed by the action of voltagedependent ion channels. The localization of specific populations of ion channels with distinct functional properties at discrete sites in neurons dramatically impacts excitability and synaptic transmission. Molecular cloning studies have revealed a large family of genes encoding voltage-dependent ion channel principal and auxiliary subunits, most of which are expressed in mammalian central neurons. Much recent effort has focused on determining which of these subunits coassemble into native neuronal channel complexes, and the cellular and subcellular distributions of these complexes, as a crucial step in understanding the contribution of these channels to specific aspects of neuronal function. Here we review progress made on recent studies aimed to determine the cellular and subcellular distribution of specific ion channel subunits in mammalian brain neurons using in situ hybridization and immunohistochemistry. We also discuss the repertoire of ion channel subunits in specific neuronal compartments and implications for neuronal physiology. Finally, we discuss the emerging mechanisms for determining the discrete subcellular distributions observed for many neuronal ion channels.",
author = "Helene Vacher and Mohapatra, {Durga P.} and James Trimmer",
year = "2008",
month = "10",
doi = "10.1152/physrev.00002.2008",
language = "English (US)",
volume = "88",
pages = "1407--1447",
journal = "Physiological Reviews",
issn = "0031-9333",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - Localization and targeting of voltage-dependent ion channels in mammalian central neurons

AU - Vacher, Helene

AU - Mohapatra, Durga P.

AU - Trimmer, James

PY - 2008/10

Y1 - 2008/10

N2 - The intrinsic electrical properties and the synaptic input-output relationships of neurons are governed by the action of voltagedependent ion channels. The localization of specific populations of ion channels with distinct functional properties at discrete sites in neurons dramatically impacts excitability and synaptic transmission. Molecular cloning studies have revealed a large family of genes encoding voltage-dependent ion channel principal and auxiliary subunits, most of which are expressed in mammalian central neurons. Much recent effort has focused on determining which of these subunits coassemble into native neuronal channel complexes, and the cellular and subcellular distributions of these complexes, as a crucial step in understanding the contribution of these channels to specific aspects of neuronal function. Here we review progress made on recent studies aimed to determine the cellular and subcellular distribution of specific ion channel subunits in mammalian brain neurons using in situ hybridization and immunohistochemistry. We also discuss the repertoire of ion channel subunits in specific neuronal compartments and implications for neuronal physiology. Finally, we discuss the emerging mechanisms for determining the discrete subcellular distributions observed for many neuronal ion channels.

AB - The intrinsic electrical properties and the synaptic input-output relationships of neurons are governed by the action of voltagedependent ion channels. The localization of specific populations of ion channels with distinct functional properties at discrete sites in neurons dramatically impacts excitability and synaptic transmission. Molecular cloning studies have revealed a large family of genes encoding voltage-dependent ion channel principal and auxiliary subunits, most of which are expressed in mammalian central neurons. Much recent effort has focused on determining which of these subunits coassemble into native neuronal channel complexes, and the cellular and subcellular distributions of these complexes, as a crucial step in understanding the contribution of these channels to specific aspects of neuronal function. Here we review progress made on recent studies aimed to determine the cellular and subcellular distribution of specific ion channel subunits in mammalian brain neurons using in situ hybridization and immunohistochemistry. We also discuss the repertoire of ion channel subunits in specific neuronal compartments and implications for neuronal physiology. Finally, we discuss the emerging mechanisms for determining the discrete subcellular distributions observed for many neuronal ion channels.

UR - http://www.scopus.com/inward/record.url?scp=55949121591&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=55949121591&partnerID=8YFLogxK

U2 - 10.1152/physrev.00002.2008

DO - 10.1152/physrev.00002.2008

M3 - Article

C2 - 18923186

AN - SCOPUS:55949121591

VL - 88

SP - 1407

EP - 1447

JO - Physiological Reviews

JF - Physiological Reviews

SN - 0031-9333

IS - 4

ER -