Local Regulation of L-Type Ca2+ Channel Sparklets in Arterial Smooth Muscle

Manuel F Navedo, Gregory C. Amberg

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This review addresses the latest advances in our understanding of the regulation of a novel Ca2+ signal called L-type Ca2+ channel sparklets in arterial smooth muscle. L-type Ca2+ channel sparklets are elementary Ca2+ influx events produced by the opening of a single or a small cluster of L-type Ca2+ channels. These Ca2+ signals were first visualized in the vasculature in arterial smooth muscle cells. In these cells, L-type Ca2+ channel sparklets display two functionally distinct gating modalities that regulate local and global [Ca2+]i. The activity of L-type Ca2+ channel sparklets varies regionally within a cell depending on the dynamic activity of a cohort of protein kinases and phosphatases recruited to L-type Ca2+ channels in the arterial smooth muscle sarcolemma in a complex coordinated by the scaffolding molecule AKAP150. We also described a mechanism whereby clusters of L-type Ca2+ channels gate cooperatively to amplify intracellular Ca2+ signals with likely pathological consequences.

Original languageEnglish (US)
Pages (from-to)290-298
Number of pages9
JournalMicrocirculation
Volume20
Issue number4
DOIs
StatePublished - May 2013

Fingerprint

Smooth Muscle
Sarcolemma
Phosphoprotein Phosphatases
Protein Kinases
Smooth Muscle Myocytes

Keywords

  • AKAP150
  • Coupled gating
  • Hypertension
  • TIRF microscopy
  • Type II diabetes

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Local Regulation of L-Type Ca2+ Channel Sparklets in Arterial Smooth Muscle. / Navedo, Manuel F; Amberg, Gregory C.

In: Microcirculation, Vol. 20, No. 4, 05.2013, p. 290-298.

Research output: Contribution to journalArticle

@article{1d382f839477406d8aac014a93e5b8d8,
title = "Local Regulation of L-Type Ca2+ Channel Sparklets in Arterial Smooth Muscle",
abstract = "This review addresses the latest advances in our understanding of the regulation of a novel Ca2+ signal called L-type Ca2+ channel sparklets in arterial smooth muscle. L-type Ca2+ channel sparklets are elementary Ca2+ influx events produced by the opening of a single or a small cluster of L-type Ca2+ channels. These Ca2+ signals were first visualized in the vasculature in arterial smooth muscle cells. In these cells, L-type Ca2+ channel sparklets display two functionally distinct gating modalities that regulate local and global [Ca2+]i. The activity of L-type Ca2+ channel sparklets varies regionally within a cell depending on the dynamic activity of a cohort of protein kinases and phosphatases recruited to L-type Ca2+ channels in the arterial smooth muscle sarcolemma in a complex coordinated by the scaffolding molecule AKAP150. We also described a mechanism whereby clusters of L-type Ca2+ channels gate cooperatively to amplify intracellular Ca2+ signals with likely pathological consequences.",
keywords = "AKAP150, Coupled gating, Hypertension, TIRF microscopy, Type II diabetes",
author = "Navedo, {Manuel F} and Amberg, {Gregory C.}",
year = "2013",
month = "5",
doi = "10.1111/micc.12021",
language = "English (US)",
volume = "20",
pages = "290--298",
journal = "Microcirculation",
issn = "1073-9688",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Local Regulation of L-Type Ca2+ Channel Sparklets in Arterial Smooth Muscle

AU - Navedo, Manuel F

AU - Amberg, Gregory C.

PY - 2013/5

Y1 - 2013/5

N2 - This review addresses the latest advances in our understanding of the regulation of a novel Ca2+ signal called L-type Ca2+ channel sparklets in arterial smooth muscle. L-type Ca2+ channel sparklets are elementary Ca2+ influx events produced by the opening of a single or a small cluster of L-type Ca2+ channels. These Ca2+ signals were first visualized in the vasculature in arterial smooth muscle cells. In these cells, L-type Ca2+ channel sparklets display two functionally distinct gating modalities that regulate local and global [Ca2+]i. The activity of L-type Ca2+ channel sparklets varies regionally within a cell depending on the dynamic activity of a cohort of protein kinases and phosphatases recruited to L-type Ca2+ channels in the arterial smooth muscle sarcolemma in a complex coordinated by the scaffolding molecule AKAP150. We also described a mechanism whereby clusters of L-type Ca2+ channels gate cooperatively to amplify intracellular Ca2+ signals with likely pathological consequences.

AB - This review addresses the latest advances in our understanding of the regulation of a novel Ca2+ signal called L-type Ca2+ channel sparklets in arterial smooth muscle. L-type Ca2+ channel sparklets are elementary Ca2+ influx events produced by the opening of a single or a small cluster of L-type Ca2+ channels. These Ca2+ signals were first visualized in the vasculature in arterial smooth muscle cells. In these cells, L-type Ca2+ channel sparklets display two functionally distinct gating modalities that regulate local and global [Ca2+]i. The activity of L-type Ca2+ channel sparklets varies regionally within a cell depending on the dynamic activity of a cohort of protein kinases and phosphatases recruited to L-type Ca2+ channels in the arterial smooth muscle sarcolemma in a complex coordinated by the scaffolding molecule AKAP150. We also described a mechanism whereby clusters of L-type Ca2+ channels gate cooperatively to amplify intracellular Ca2+ signals with likely pathological consequences.

KW - AKAP150

KW - Coupled gating

KW - Hypertension

KW - TIRF microscopy

KW - Type II diabetes

UR - http://www.scopus.com/inward/record.url?scp=84877648743&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877648743&partnerID=8YFLogxK

U2 - 10.1111/micc.12021

DO - 10.1111/micc.12021

M3 - Article

C2 - 23116449

AN - SCOPUS:84877648743

VL - 20

SP - 290

EP - 298

JO - Microcirculation

JF - Microcirculation

SN - 1073-9688

IS - 4

ER -