Abstract
Objective-We previously demonstrated that upregulation of intermediate-conductance Ca 2+-activated K+ channels (K Ca3.1) is necessary for mitogen-induced phenotypic modulation in isolated porcine coronary smooth muscle cells (SMCs). The objective of the present study was to determine the role of K Ca3.1 in the regulation of coronary SMC phenotypic modulation in vivo using a swine model of postangioplasty restenosis. Methods and Results-Balloon angioplasty was performed on coronary arteries of swine using either noncoated or balloons coated with the specific K Ca3.1 blocker TRAM-34. Expression of K Ca3.1, c-jun, c-fos, repressor element-1 silencing transcription factor (REST), smooth muscle myosin heavy chain (SMMHC), and myocardin was measured using qRT-PCR in isolated medial cells 2 hours and 2 days postangioplasty. K Ca3.1, c-jun, and c-fos mRNA levels were increased 2 hours postangioplasty, whereas REST expression decreased. SMMHC expression was unchanged at 2 hours, but decreased 2 days postangioplasty. Use of TRAM-34 coated balloons prevented K Ca3.1 upregulation and REST downregulation at 2 hours, SMMHC and myocardin downregulation at 2 days, and attenuated subsequent restenosis 14 and 28 days postangioplasty. Immunohistochemical analysis demonstrated corresponding changes at the protein level. Conclusion-Blockade of K Ca3.1 by delivery of TRAM-34 via balloon catheter prevented smooth muscle phenotypic modulation and limited subsequent restenosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1084-1089 |
| Number of pages | 6 |
| Journal | Arteriosclerosis, Thrombosis, and Vascular Biology |
| Volume | 28 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1 2008 |
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Keywords
- Balloon angioplasty
- Coronary smooth muscle
- K 3.1
- Phenotypic modulation
- TRAM-34
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Local delivery of the K Ca3.1 blocker, TRAM-34, prevents acute angioplasty-induced coronary smooth muscle phenotypic modulation and limits stenosis. / Tharp, D. L.; Wamhoff, B. R.; Wulff, Heike; Raman, G.; Cheong, A.; Bowles, D. K.
In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 28, No. 6, 01.06.2008, p. 1084-1089.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Local delivery of the K Ca3.1 blocker, TRAM-34, prevents acute angioplasty-induced coronary smooth muscle phenotypic modulation and limits stenosis
AU - Tharp, D. L.
AU - Wamhoff, B. R.
AU - Wulff, Heike
AU - Raman, G.
AU - Cheong, A.
AU - Bowles, D. K.
PY - 2008/6/1
Y1 - 2008/6/1
N2 - Objective-We previously demonstrated that upregulation of intermediate-conductance Ca 2+-activated K+ channels (K Ca3.1) is necessary for mitogen-induced phenotypic modulation in isolated porcine coronary smooth muscle cells (SMCs). The objective of the present study was to determine the role of K Ca3.1 in the regulation of coronary SMC phenotypic modulation in vivo using a swine model of postangioplasty restenosis. Methods and Results-Balloon angioplasty was performed on coronary arteries of swine using either noncoated or balloons coated with the specific K Ca3.1 blocker TRAM-34. Expression of K Ca3.1, c-jun, c-fos, repressor element-1 silencing transcription factor (REST), smooth muscle myosin heavy chain (SMMHC), and myocardin was measured using qRT-PCR in isolated medial cells 2 hours and 2 days postangioplasty. K Ca3.1, c-jun, and c-fos mRNA levels were increased 2 hours postangioplasty, whereas REST expression decreased. SMMHC expression was unchanged at 2 hours, but decreased 2 days postangioplasty. Use of TRAM-34 coated balloons prevented K Ca3.1 upregulation and REST downregulation at 2 hours, SMMHC and myocardin downregulation at 2 days, and attenuated subsequent restenosis 14 and 28 days postangioplasty. Immunohistochemical analysis demonstrated corresponding changes at the protein level. Conclusion-Blockade of K Ca3.1 by delivery of TRAM-34 via balloon catheter prevented smooth muscle phenotypic modulation and limited subsequent restenosis.
AB - Objective-We previously demonstrated that upregulation of intermediate-conductance Ca 2+-activated K+ channels (K Ca3.1) is necessary for mitogen-induced phenotypic modulation in isolated porcine coronary smooth muscle cells (SMCs). The objective of the present study was to determine the role of K Ca3.1 in the regulation of coronary SMC phenotypic modulation in vivo using a swine model of postangioplasty restenosis. Methods and Results-Balloon angioplasty was performed on coronary arteries of swine using either noncoated or balloons coated with the specific K Ca3.1 blocker TRAM-34. Expression of K Ca3.1, c-jun, c-fos, repressor element-1 silencing transcription factor (REST), smooth muscle myosin heavy chain (SMMHC), and myocardin was measured using qRT-PCR in isolated medial cells 2 hours and 2 days postangioplasty. K Ca3.1, c-jun, and c-fos mRNA levels were increased 2 hours postangioplasty, whereas REST expression decreased. SMMHC expression was unchanged at 2 hours, but decreased 2 days postangioplasty. Use of TRAM-34 coated balloons prevented K Ca3.1 upregulation and REST downregulation at 2 hours, SMMHC and myocardin downregulation at 2 days, and attenuated subsequent restenosis 14 and 28 days postangioplasty. Immunohistochemical analysis demonstrated corresponding changes at the protein level. Conclusion-Blockade of K Ca3.1 by delivery of TRAM-34 via balloon catheter prevented smooth muscle phenotypic modulation and limited subsequent restenosis.
KW - Balloon angioplasty
KW - Coronary smooth muscle
KW - K 3.1
KW - Phenotypic modulation
KW - TRAM-34
UR - http://www.scopus.com/inward/record.url?scp=44849100851&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44849100851&partnerID=8YFLogxK
U2 - 10.1161/ATVBAHA.107.155796
DO - 10.1161/ATVBAHA.107.155796
M3 - Article
C2 - 18309114
AN - SCOPUS:44849100851
VL - 28
SP - 1084
EP - 1089
JO - Arteriosclerosis, Thrombosis, and Vascular Biology
JF - Arteriosclerosis, Thrombosis, and Vascular Biology
SN - 1079-5642
IS - 6
ER -