Abstract
To understand local antibody production to dietary protein antigens in the gut, the reactivity of the monoclonal antibodies (mAbs) from Peyer’s patches of BALB/c mice raised against orally administered hen egg lysozyme (HEL) was studied. These mAbs were of IgG1 (7 clones), IgA (5 clones) and IgM (13 clones) isotypes. Some of the HEL-binding mAbs preferentially reacted with reduced, carboxy-methylated HEL, rather than with native HEL. MAbs of the IgA and IgM isotypes had cross-reactivity with other unrelated environmental antigens such as E. coli, single-strand DNA, and soluble components of mouse food. In contrast, the IgG1 mAbs did not cross-react with these antigens. The average of the Kd values for HEL of these mAbs was in the order of 10-6 M, which is moderately higher than those of mAbs from the preimmune repertoire. These results suggest that, under normal physiological conditions, orally administered dietary proteins predominantly induce the local production of polyreactive IgA/IgM antibodies cross-reacting with environmental luminal antigens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2123-2129 |
| Number of pages | 7 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 63 |
| Issue number | 12 |
| DOIs | |
| State | Published - Jan 1 1999 |
| Externally published | Yes |
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Keywords
- Lysozyme
- Mucosal IgA response
- Peyer’s patches
- Polyreactive antibody
ASJC Scopus subject areas
- Analytical Chemistry
- Biotechnology
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Local Antibody Response in Peyer’s Patches to the Orally Administered Dietary Protein Antigen. / Shimoda, Michiko; Inoue, Yuri; Azuma, Norihiro; Kanno, Choemon.
In: Bioscience, Biotechnology and Biochemistry, Vol. 63, No. 12, 01.01.1999, p. 2123-2129.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Local Antibody Response in Peyer’s Patches to the Orally Administered Dietary Protein Antigen
AU - Shimoda, Michiko
AU - Inoue, Yuri
AU - Azuma, Norihiro
AU - Kanno, Choemon
PY - 1999/1/1
Y1 - 1999/1/1
N2 - To understand local antibody production to dietary protein antigens in the gut, the reactivity of the monoclonal antibodies (mAbs) from Peyer’s patches of BALB/c mice raised against orally administered hen egg lysozyme (HEL) was studied. These mAbs were of IgG1 (7 clones), IgA (5 clones) and IgM (13 clones) isotypes. Some of the HEL-binding mAbs preferentially reacted with reduced, carboxy-methylated HEL, rather than with native HEL. MAbs of the IgA and IgM isotypes had cross-reactivity with other unrelated environmental antigens such as E. coli, single-strand DNA, and soluble components of mouse food. In contrast, the IgG1 mAbs did not cross-react with these antigens. The average of the Kd values for HEL of these mAbs was in the order of 10-6 M, which is moderately higher than those of mAbs from the preimmune repertoire. These results suggest that, under normal physiological conditions, orally administered dietary proteins predominantly induce the local production of polyreactive IgA/IgM antibodies cross-reacting with environmental luminal antigens.
AB - To understand local antibody production to dietary protein antigens in the gut, the reactivity of the monoclonal antibodies (mAbs) from Peyer’s patches of BALB/c mice raised against orally administered hen egg lysozyme (HEL) was studied. These mAbs were of IgG1 (7 clones), IgA (5 clones) and IgM (13 clones) isotypes. Some of the HEL-binding mAbs preferentially reacted with reduced, carboxy-methylated HEL, rather than with native HEL. MAbs of the IgA and IgM isotypes had cross-reactivity with other unrelated environmental antigens such as E. coli, single-strand DNA, and soluble components of mouse food. In contrast, the IgG1 mAbs did not cross-react with these antigens. The average of the Kd values for HEL of these mAbs was in the order of 10-6 M, which is moderately higher than those of mAbs from the preimmune repertoire. These results suggest that, under normal physiological conditions, orally administered dietary proteins predominantly induce the local production of polyreactive IgA/IgM antibodies cross-reacting with environmental luminal antigens.
KW - Lysozyme
KW - Mucosal IgA response
KW - Peyer’s patches
KW - Polyreactive antibody
UR - http://www.scopus.com/inward/record.url?scp=0033253941&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033253941&partnerID=8YFLogxK
U2 - 10.1271/bbb.63.2123
DO - 10.1271/bbb.63.2123
M3 - Article
C2 - 10664845
AN - SCOPUS:0033253941
VL - 63
SP - 2123
EP - 2129
JO - Bioscience, biotechnology, and biochemistry
JF - Bioscience, biotechnology, and biochemistry
SN - 0916-8451
IS - 12
ER -