Local Antibody Response in Peyer’s Patches to the Orally Administered Dietary Protein Antigen

Michiko Shimoda, Yuri Inoue, Norihiro Azuma, Choemon Kanno

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


To understand local antibody production to dietary protein antigens in the gut, the reactivity of the monoclonal antibodies (mAbs) from Peyer’s patches of BALB/c mice raised against orally administered hen egg lysozyme (HEL) was studied. These mAbs were of IgG1 (7 clones), IgA (5 clones) and IgM (13 clones) isotypes. Some of the HEL-binding mAbs preferentially reacted with reduced, carboxy-methylated HEL, rather than with native HEL. MAbs of the IgA and IgM isotypes had cross-reactivity with other unrelated environmental antigens such as E. coli, single-strand DNA, and soluble components of mouse food. In contrast, the IgG1 mAbs did not cross-react with these antigens. The average of the Kd values for HEL of these mAbs was in the order of 10-6 M, which is moderately higher than those of mAbs from the preimmune repertoire. These results suggest that, under normal physiological conditions, orally administered dietary proteins predominantly induce the local production of polyreactive IgA/IgM antibodies cross-reacting with environmental luminal antigens.

Original languageEnglish (US)
Pages (from-to)2123-2129
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Issue number12
StatePublished - Jan 1 1999
Externally publishedYes


  • Lysozyme
  • Mucosal IgA response
  • Peyer’s patches
  • Polyreactive antibody

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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