Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes

Hernan Lorenzi; Asis Khan; Michael S. Behnke; Sivaranjani Namasivayam; Lakshmipuram S. Swapna; Michalis Hadjithomas; Svetlana Karamycheva; Deborah Pinney; Brian P. Brunk; James W. Ajioka; Daniel Ajzenberg; John C. Boothroyd; Jon P. Boyle; Marie L. Dardé; Maria A. Diaz-Miranda; Jitender P. Dubey; Heather Fritz; Solange M. Gennari; Brian D. Gregory; Kami Kim; Jeroen Saeij; Chunlei Su; Michael W. White; Xing Quan Zhu; Daniel K. Howe; Benjamin M. Rosenthal; Michael E. Grigg; John Parkinson; Liang Liu; Jessica C. Kissinger; David S. Roos; L. David Sibley

doi:10.1038/ncomms10147

Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes

Hernan Lorenzi, Asis Khan, Michael S. Behnke, Sivaranjani Namasivayam, Lakshmipuram S. Swapna, Michalis Hadjithomas, Svetlana Karamycheva, Deborah Pinney, Brian P. Brunk, James W. Ajioka, Daniel Ajzenberg, John C. Boothroyd, Jon P. Boyle, Marie L. Dardé, Maria A. Diaz-Miranda, Jitender P. Dubey, Heather Fritz, Solange M. Gennari, Brian D. Gregory, Kami KimJeroen Saeij, Chunlei Su, Michael W. White, Xing Quan Zhu, Daniel K. Howe, Benjamin M. Rosenthal, Michael E. Grigg, John Parkinson, Liang Liu, Jessica C. Kissinger, David S. Roos, L. David Sibley

Pathology, Microbiology and Immunology

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Abstract

Toxoplasma gondii is among the most prevalent parasites worldwide, infecting many wild and domestic animals and causing zoonotic infections in humans. T. gondii differs substantially in its broad distribution from closely related parasites that typically have narrow, specialized host ranges. To elucidate the genetic basis for these differences, we compared the genomes of 62 globally distributed T. gondii isolates to several closely related coccidian parasites. Our findings reveal that tandem amplification and diversification of secretory pathogenesis determinants is the primary feature that distinguishes the closely related genomes of these biologically diverse parasites. We further show that the unusual population structure of T. gondii is characterized by clade-specific inheritance of large conserved haploblocks that are significantly enriched in tandemly clustered secretory pathogenesis determinants. The shared inheritance of these conserved haploblocks, which show a different ancestry than the genome as a whole, may thus influence transmission, host range and pathogenicity.

Original language	English (US)
Article number	10147
Journal	Nature Communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms10147
State	Published - Jan 7 2016

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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Lorenzi, H., Khan, A., Behnke, M. S., Namasivayam, S., Swapna, L. S., Hadjithomas, M., Karamycheva, S., Pinney, D., Brunk, B. P., Ajioka, J. W., Ajzenberg, D., Boothroyd, J. C., Boyle, J. P., Dardé, M. L., Diaz-Miranda, M. A., Dubey, J. P., Fritz, H., Gennari, S. M., Gregory, B. D., ... Sibley, L. D. (2016). Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes. Nature Communications, 7, [10147]. https://doi.org/10.1038/ncomms10147

Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes. / Lorenzi, Hernan; Khan, Asis; Behnke, Michael S.; Namasivayam, Sivaranjani; Swapna, Lakshmipuram S.; Hadjithomas, Michalis; Karamycheva, Svetlana; Pinney, Deborah; Brunk, Brian P.; Ajioka, James W.; Ajzenberg, Daniel; Boothroyd, John C.; Boyle, Jon P.; Dardé, Marie L.; Diaz-Miranda, Maria A.; Dubey, Jitender P.; Fritz, Heather; Gennari, Solange M.; Gregory, Brian D.; Kim, Kami; Saeij, Jeroen; Su, Chunlei; White, Michael W.; Zhu, Xing Quan; Howe, Daniel K.; Rosenthal, Benjamin M.; Grigg, Michael E.; Parkinson, John; Liu, Liang; Kissinger, Jessica C.; Roos, David S.; Sibley, L. David.

In: Nature Communications, Vol. 7, 10147, 07.01.2016.

Research output: Contribution to journal › Article › peer-review

Lorenzi, H, Khan, A, Behnke, MS, Namasivayam, S, Swapna, LS, Hadjithomas, M, Karamycheva, S, Pinney, D, Brunk, BP, Ajioka, JW, Ajzenberg, D, Boothroyd, JC, Boyle, JP, Dardé, ML, Diaz-Miranda, MA, Dubey, JP, Fritz, H, Gennari, SM, Gregory, BD, Kim, K, Saeij, J, Su, C, White, MW, Zhu, XQ, Howe, DK, Rosenthal, BM, Grigg, ME, Parkinson, J, Liu, L, Kissinger, JC, Roos, DS & Sibley, LD 2016, 'Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes', Nature Communications, vol. 7, 10147. https://doi.org/10.1038/ncomms10147

Lorenzi, Hernan ; Khan, Asis ; Behnke, Michael S. ; Namasivayam, Sivaranjani ; Swapna, Lakshmipuram S. ; Hadjithomas, Michalis ; Karamycheva, Svetlana ; Pinney, Deborah ; Brunk, Brian P. ; Ajioka, James W. ; Ajzenberg, Daniel ; Boothroyd, John C. ; Boyle, Jon P. ; Dardé, Marie L. ; Diaz-Miranda, Maria A. ; Dubey, Jitender P. ; Fritz, Heather ; Gennari, Solange M. ; Gregory, Brian D. ; Kim, Kami ; Saeij, Jeroen ; Su, Chunlei ; White, Michael W. ; Zhu, Xing Quan ; Howe, Daniel K. ; Rosenthal, Benjamin M. ; Grigg, Michael E. ; Parkinson, John ; Liu, Liang ; Kissinger, Jessica C. ; Roos, David S. ; Sibley, L. David. / Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes. In: Nature Communications. 2016 ; Vol. 7.

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abstract = "Toxoplasma gondii is among the most prevalent parasites worldwide, infecting many wild and domestic animals and causing zoonotic infections in humans. T. gondii differs substantially in its broad distribution from closely related parasites that typically have narrow, specialized host ranges. To elucidate the genetic basis for these differences, we compared the genomes of 62 globally distributed T. gondii isolates to several closely related coccidian parasites. Our findings reveal that tandem amplification and diversification of secretory pathogenesis determinants is the primary feature that distinguishes the closely related genomes of these biologically diverse parasites. We further show that the unusual population structure of T. gondii is characterized by clade-specific inheritance of large conserved haploblocks that are significantly enriched in tandemly clustered secretory pathogenesis determinants. The shared inheritance of these conserved haploblocks, which show a different ancestry than the genome as a whole, may thus influence transmission, host range and pathogenicity.",

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AU - Saeij, Jeroen

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AU - White, Michael W.

AU - Zhu, Xing Quan

AU - Howe, Daniel K.

AU - Rosenthal, Benjamin M.

AU - Grigg, Michael E.

AU - Parkinson, John

AU - Liu, Liang

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Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes

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