LncRNA TUG1 regulates FGF1 to enhance endothelial differentiation of adipose-derived stem cells by sponging miR-143

Ya Nan Xue, Yu Yan, Zi Zi Chen, Jia Chen, Feng Jie Tang, Hui Qing Xie, Shi Jie Tang, Ke Cao, Xiao Zhou, Ai Jun Wang, Jian Da Zhou

Research output: Contribution to journalArticle

Abstract

Adipose-derived stem cells (ADSCs) have emerged as a cell source for regeneration medicine. ADSCs possess the capacity to differentiate into endothelial cells and serve an essential role in vascular development and function. LncRNA taurine upregulated gene 1 (TUG1) has recently been linked with angiogenesis in hepatoblastoma. However, the roles of TUG1 in endothelial differentiation of ADSCs remain unidentified. Human adipose-derived stem cells (hADSCs) were obtained and characterized by flow cytometry, Oil red O and Alizarin Red staining. HADSCs were maintained in the endothelial differentiation medium and the expressions of TUG1, miR-143, and FGF1 were examined by qRT-PCR. To assess endothelial differentiation, the expressions of CD31, von Willebrand factor (vWF), VE-cadherin were examined by Western blot analysis, qRT-PCR, and immunofluorescence. Tube formation in Matrigel was examined. The interactions between TUG1 and miR-143, miR-143 and FGF1 were validated by luciferase assays. During the endothelial differentiation process, TUG1 and FGF1 were upregulated, whereas miR-143 was downregulated. TUG1 overexpression downregulated miR-143, upregulated FGF1, CD31, vWF, and VE-cadherin, and enhanced capillary tube formation. Luciferase assays showed that TUG1 interacted with miR-143, and FGF1 was a direct target of miR-143. Furthermore, the enhancement of endothelial differentiation induced by TUG1 overexpression was abolished by miR-143 overexpression. Our findings implicated that lncRNA TUG1 promoted endothelial differentiation of ADSCs by regulating the miR-143/FGF1 axis.

Original languageEnglish (US)
Pages (from-to)19087-19097
Number of pages11
JournalJournal of Cellular Biochemistry
Volume120
Issue number11
DOIs
StatePublished - Nov 1 2019

Fingerprint

Fibroblast Growth Factor 1
Taurine
Stem cells
Stem Cells
Genes
von Willebrand Factor
Luciferases
Assays
Down-Regulation
Long Noncoding RNA
Hepatoblastoma
Capillary tubes
Polymerase Chain Reaction
Flow cytometry
Endothelial cells
Medicine
Fluorescent Antibody Technique
Blood Vessels
Regeneration
Flow Cytometry

Keywords

  • adipose-deprived stem cells
  • endothelial differentiation
  • FGF1
  • LncRNA TUG1
  • miR-143

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

LncRNA TUG1 regulates FGF1 to enhance endothelial differentiation of adipose-derived stem cells by sponging miR-143. / Xue, Ya Nan; Yan, Yu; Chen, Zi Zi; Chen, Jia; Tang, Feng Jie; Xie, Hui Qing; Tang, Shi Jie; Cao, Ke; Zhou, Xiao; Wang, Ai Jun; Zhou, Jian Da.

In: Journal of Cellular Biochemistry, Vol. 120, No. 11, 01.11.2019, p. 19087-19097.

Research output: Contribution to journalArticle

Xue, YN, Yan, Y, Chen, ZZ, Chen, J, Tang, FJ, Xie, HQ, Tang, SJ, Cao, K, Zhou, X, Wang, AJ & Zhou, JD 2019, 'LncRNA TUG1 regulates FGF1 to enhance endothelial differentiation of adipose-derived stem cells by sponging miR-143', Journal of Cellular Biochemistry, vol. 120, no. 11, pp. 19087-19097. https://doi.org/10.1002/jcb.29232
Xue, Ya Nan ; Yan, Yu ; Chen, Zi Zi ; Chen, Jia ; Tang, Feng Jie ; Xie, Hui Qing ; Tang, Shi Jie ; Cao, Ke ; Zhou, Xiao ; Wang, Ai Jun ; Zhou, Jian Da. / LncRNA TUG1 regulates FGF1 to enhance endothelial differentiation of adipose-derived stem cells by sponging miR-143. In: Journal of Cellular Biochemistry. 2019 ; Vol. 120, No. 11. pp. 19087-19097.
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T1 - LncRNA TUG1 regulates FGF1 to enhance endothelial differentiation of adipose-derived stem cells by sponging miR-143

AU - Xue, Ya Nan

AU - Yan, Yu

AU - Chen, Zi Zi

AU - Chen, Jia

AU - Tang, Feng Jie

AU - Xie, Hui Qing

AU - Tang, Shi Jie

AU - Cao, Ke

AU - Zhou, Xiao

AU - Wang, Ai Jun

AU - Zhou, Jian Da

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KW - adipose-deprived stem cells

KW - endothelial differentiation

KW - FGF1

KW - LncRNA TUG1

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