TY - JOUR
T1 - Liver fatty acid composition correlates with body fat and sex in a multigenic mouse model of obesity
AU - Phinney, Stephen D.
AU - Fisler, Janis S.
AU - Tang, Anna B.
AU - Warden, Craig H
PY - 1994/7
Y1 - 1994/7
N2 - To determine whether there is altered liver lipid-fraction fatty acid distribution in a multigenic obese mouse model, we examined livers from eight lean (0.2-4.2% carcass fat), seven intermediate (5.7-13.8%), and five obese (20.2-48.7%) backcross progeny [(C57BL/6J x Mus spretus) x C57BL/6J] aged 2- 3 mo. Thirteen males and seven females were fed a nonpurified stock diet. Liver lipid fractions were separated and fatty acids quantitated by thin- layer and gas chromatography. There was a significant effect of obesity on 18:2ω6 in liver phospholipids (PL), cholesteryl esters, and triglycerides. PL 18:2ω6 was negatively correlated with carcass fat (r = -0.74, P < 0.001); 20:3ω6 was elevated in PL with increased obesity (P < 0.0001), and was correlated with carcass fat (r = 0.92, P < 0.0001); and 20:4ω6 in PL did not differ with obesity status. PL 20:3ω6 and 20:4ω6 were lower in males (P < 0.01 and 0.02, respectively) than in females. We conclude that obesity and sex affect distribution of ω6 essential fatty acids in mouse liver lipid fractions.
AB - To determine whether there is altered liver lipid-fraction fatty acid distribution in a multigenic obese mouse model, we examined livers from eight lean (0.2-4.2% carcass fat), seven intermediate (5.7-13.8%), and five obese (20.2-48.7%) backcross progeny [(C57BL/6J x Mus spretus) x C57BL/6J] aged 2- 3 mo. Thirteen males and seven females were fed a nonpurified stock diet. Liver lipid fractions were separated and fatty acids quantitated by thin- layer and gas chromatography. There was a significant effect of obesity on 18:2ω6 in liver phospholipids (PL), cholesteryl esters, and triglycerides. PL 18:2ω6 was negatively correlated with carcass fat (r = -0.74, P < 0.001); 20:3ω6 was elevated in PL with increased obesity (P < 0.0001), and was correlated with carcass fat (r = 0.92, P < 0.0001); and 20:4ω6 in PL did not differ with obesity status. PL 20:3ω6 and 20:4ω6 were lower in males (P < 0.01 and 0.02, respectively) than in females. We conclude that obesity and sex affect distribution of ω6 essential fatty acids in mouse liver lipid fractions.
UR - http://www.scopus.com/inward/record.url?scp=0028284235&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028284235&partnerID=8YFLogxK
M3 - Article
C2 - 8017339
AN - SCOPUS:0028284235
VL - 60
SP - 61
EP - 67
JO - American Journal of Clinical Nutrition
JF - American Journal of Clinical Nutrition
SN - 0002-9165
IS - 1
ER -