Liver fatty acid composition correlates with body fat and sex in a multigenic mouse model of obesity

Stephen D. Phinney, Janis S. Fisler, Anna B. Tang, Craig H Warden

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

To determine whether there is altered liver lipid-fraction fatty acid distribution in a multigenic obese mouse model, we examined livers from eight lean (0.2-4.2% carcass fat), seven intermediate (5.7-13.8%), and five obese (20.2-48.7%) backcross progeny [(C57BL/6J x Mus spretus) x C57BL/6J] aged 2- 3 mo. Thirteen males and seven females were fed a nonpurified stock diet. Liver lipid fractions were separated and fatty acids quantitated by thin- layer and gas chromatography. There was a significant effect of obesity on 18:2ω6 in liver phospholipids (PL), cholesteryl esters, and triglycerides. PL 18:2ω6 was negatively correlated with carcass fat (r = -0.74, P < 0.001); 20:3ω6 was elevated in PL with increased obesity (P < 0.0001), and was correlated with carcass fat (r = 0.92, P < 0.0001); and 20:4ω6 in PL did not differ with obesity status. PL 20:3ω6 and 20:4ω6 were lower in males (P < 0.01 and 0.02, respectively) than in females. We conclude that obesity and sex affect distribution of ω6 essential fatty acids in mouse liver lipid fractions.

Original languageEnglish (US)
Pages (from-to)61-67
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume60
Issue number1
StatePublished - Jul 1994
Externally publishedYes

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body fat
Adipose Tissue
Phospholipids
obesity
Fatty Acids
Obesity
animal models
fatty acid composition
phospholipids
liver
gender
Liver
lipids
Fats
Lipids
Obese Mice
Essential Fatty Acids
Sex Distribution
Cholesterol Esters
fatty acids

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Liver fatty acid composition correlates with body fat and sex in a multigenic mouse model of obesity. / Phinney, Stephen D.; Fisler, Janis S.; Tang, Anna B.; Warden, Craig H.

In: American Journal of Clinical Nutrition, Vol. 60, No. 1, 07.1994, p. 61-67.

Research output: Contribution to journalArticle

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