Live cell imaging of messenger RNA co-localization with mitochondria

Philip J. Santangelo; Nitin Nitin; Gang Bao

Live cell imaging of messenger RNA co-localization with mitochondria

Philip J. Santangelo, Nitin Nitin, Gang Bao

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The intracellular distribution and specific organelle association of mRNA may play essential roles in gene translation and RNA-protein interactions. Here we report the direct visualization of subcellular localization of K-ras and GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) mRNAs in live HDF cells using molecular beacons. Unexpectedly, we found that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive positive and negative control studies were performed to confirm that the mRNA localization and colocalization patterns observed in our study are true and specific.

Original language	English (US)
Title of host publication	Proceedings of the 2005 Summer Bioengineering Conference
Pages	701-702
Number of pages	2
Volume	2005
State	Published - 2005
Externally published	Yes
Event	2005 Summer Bioengineering Conference - Vail, CO, United States Duration: Jun 22 2005 → Jun 26 2005

Other

Other	2005 Summer Bioengineering Conference
Country/Territory	United States
City	Vail, CO
Period	6/22/05 → 6/26/05

ASJC Scopus subject areas

Engineering(all)

Cite this

@inproceedings{61d70c40516d40a2b70b01cf70e156c1,

title = "Live cell imaging of messenger RNA co-localization with mitochondria",

abstract = "The intracellular distribution and specific organelle association of mRNA may play essential roles in gene translation and RNA-protein interactions. Here we report the direct visualization of subcellular localization of K-ras and GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) mRNAs in live HDF cells using molecular beacons. Unexpectedly, we found that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive positive and negative control studies were performed to confirm that the mRNA localization and colocalization patterns observed in our study are true and specific.",

author = "Santangelo, {Philip J.} and Nitin Nitin and Gang Bao",

year = "2005",

language = "English (US)",

isbn = "0974249211",

volume = "2005",

pages = "701--702",

booktitle = "Proceedings of the 2005 Summer Bioengineering Conference",

note = "2005 Summer Bioengineering Conference ; Conference date: 22-06-2005 Through 26-06-2005",

}

TY - GEN

T1 - Live cell imaging of messenger RNA co-localization with mitochondria

AU - Santangelo, Philip J.

AU - Nitin, Nitin

AU - Bao, Gang

PY - 2005

Y1 - 2005

N2 - The intracellular distribution and specific organelle association of mRNA may play essential roles in gene translation and RNA-protein interactions. Here we report the direct visualization of subcellular localization of K-ras and GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) mRNAs in live HDF cells using molecular beacons. Unexpectedly, we found that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive positive and negative control studies were performed to confirm that the mRNA localization and colocalization patterns observed in our study are true and specific.

AB - The intracellular distribution and specific organelle association of mRNA may play essential roles in gene translation and RNA-protein interactions. Here we report the direct visualization of subcellular localization of K-ras and GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) mRNAs in live HDF cells using molecular beacons. Unexpectedly, we found that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive positive and negative control studies were performed to confirm that the mRNA localization and colocalization patterns observed in our study are true and specific.

UR - http://www.scopus.com/inward/record.url?scp=33646541047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646541047&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:33646541047

SN - 0974249211

SN - 9780974249216

VL - 2005

SP - 701

EP - 702

BT - Proceedings of the 2005 Summer Bioengineering Conference

T2 - 2005 Summer Bioengineering Conference

Y2 - 22 June 2005 through 26 June 2005

ER -

Live cell imaging of messenger RNA co-localization with mitochondria

Abstract

Other

ASJC Scopus subject areas

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