Live cell imaging of messenger RNA co-localization with mitochondria

Philip J. Santangelo, Nitin Nitin, Gang Bao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The intracellular distribution and specific organelle association of mRNA may play essential roles in gene translation and RNA-protein interactions. Here we report the direct visualization of subcellular localization of K-ras and GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) mRNAs in live HDF cells using molecular beacons. Unexpectedly, we found that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive positive and negative control studies were performed to confirm that the mRNA localization and colocalization patterns observed in our study are true and specific.

Original languageEnglish (US)
Title of host publicationProceedings of the 2005 Summer Bioengineering Conference
Pages701-702
Number of pages2
Volume2005
StatePublished - 2005
Externally publishedYes
Event2005 Summer Bioengineering Conference - Vail, CO, United States
Duration: Jun 22 2005Jun 26 2005

Other

Other2005 Summer Bioengineering Conference
CountryUnited States
CityVail, CO
Period6/22/056/26/05

Fingerprint

Mitochondria
Imaging techniques
Phosphates
RNA
Visualization
Genes
Proteins
Messenger RNA
Oxidoreductases

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Santangelo, P. J., Nitin, N., & Bao, G. (2005). Live cell imaging of messenger RNA co-localization with mitochondria. In Proceedings of the 2005 Summer Bioengineering Conference (Vol. 2005, pp. 701-702)

Live cell imaging of messenger RNA co-localization with mitochondria. / Santangelo, Philip J.; Nitin, Nitin; Bao, Gang.

Proceedings of the 2005 Summer Bioengineering Conference. Vol. 2005 2005. p. 701-702.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Santangelo, PJ, Nitin, N & Bao, G 2005, Live cell imaging of messenger RNA co-localization with mitochondria. in Proceedings of the 2005 Summer Bioengineering Conference. vol. 2005, pp. 701-702, 2005 Summer Bioengineering Conference, Vail, CO, United States, 6/22/05.
Santangelo PJ, Nitin N, Bao G. Live cell imaging of messenger RNA co-localization with mitochondria. In Proceedings of the 2005 Summer Bioengineering Conference. Vol. 2005. 2005. p. 701-702
Santangelo, Philip J. ; Nitin, Nitin ; Bao, Gang. / Live cell imaging of messenger RNA co-localization with mitochondria. Proceedings of the 2005 Summer Bioengineering Conference. Vol. 2005 2005. pp. 701-702
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