Abstract
The intracellular distribution and specific organelle association of mRNA may play essential roles in gene translation and RNA-protein interactions. Here we report the direct visualization of subcellular localization of K-ras and GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) mRNAs in live HDF cells using molecular beacons. Unexpectedly, we found that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive positive and negative control studies were performed to confirm that the mRNA localization and colocalization patterns observed in our study are true and specific.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings of the 2005 Summer Bioengineering Conference |
| Pages | 701-702 |
| Number of pages | 2 |
| Volume | 2005 |
| State | Published - 2005 |
| Externally published | Yes |
| Event | 2005 Summer Bioengineering Conference - Vail, CO, United States Duration: Jun 22 2005 → Jun 26 2005 |
Other
| Other | 2005 Summer Bioengineering Conference |
|---|---|
| Country | United States |
| City | Vail, CO |
| Period | 6/22/05 → 6/26/05 |
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ASJC Scopus subject areas
- Engineering(all)
Cite this
Live cell imaging of messenger RNA co-localization with mitochondria. / Santangelo, Philip J.; Nitin, Nitin; Bao, Gang.
Proceedings of the 2005 Summer Bioengineering Conference. Vol. 2005 2005. p. 701-702.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Live cell imaging of messenger RNA co-localization with mitochondria
AU - Santangelo, Philip J.
AU - Nitin, Nitin
AU - Bao, Gang
PY - 2005
Y1 - 2005
N2 - The intracellular distribution and specific organelle association of mRNA may play essential roles in gene translation and RNA-protein interactions. Here we report the direct visualization of subcellular localization of K-ras and GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) mRNAs in live HDF cells using molecular beacons. Unexpectedly, we found that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive positive and negative control studies were performed to confirm that the mRNA localization and colocalization patterns observed in our study are true and specific.
AB - The intracellular distribution and specific organelle association of mRNA may play essential roles in gene translation and RNA-protein interactions. Here we report the direct visualization of subcellular localization of K-ras and GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) mRNAs in live HDF cells using molecular beacons. Unexpectedly, we found that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive positive and negative control studies were performed to confirm that the mRNA localization and colocalization patterns observed in our study are true and specific.
UR - http://www.scopus.com/inward/record.url?scp=33646541047&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646541047&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:33646541047
SN - 0974249211
SN - 9780974249216
VL - 2005
SP - 701
EP - 702
BT - Proceedings of the 2005 Summer Bioengineering Conference
ER -