Liquid ventilation attenuates pulmonary oxidative damage

David M. Steinhorn, Michèle C. Papo, Alexandre T. Rotta, Ahmed Aljada, Bradley P. Fuhrman, Paresh Dandona

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Purpose: Liquid perfluorochemicals reduce the production of reaction oxygen species by alveolar macrophages. We sought to determine whether the use of liquid perfluorochemicals in vivo during liquid ventilation would attenuate oxidative damage to the lung. Materials and Methods: Healthy infant piglets (n = 16) were instrumented for mechanical ventilation and received intravenous oleic acid to create an acute lung injury. The animals were assigned to a nontreatment group receiving conventional mechanical ventilation or a treatment group receiving partial liquid ventilation with a liquid perfluorochemical. Following sacrifice, the bronchoalveolar lavage and lung parenchyma were analyzed for evidence of oxidative damage to lipids and proteins by determination of TBARS and carbonylated protein residues, respectively. Results: Mortality in the control group was 50% at the completion of the study compared with no deaths in the partial liquid ventilation group (P = .025). The alveolar-arterial oxygen difference was more favorable following injury in the partial liquid ventilation group. The liquid ventilation group demonstrated a 32% reduction in TBARS (P = .043) and a 14% reduction in carbonylated protein residues (P = .061). Conclusion: These data suggest that partial liquid ventilation supports gas exchange and reduces mortality in association with a reduction in the production of reactive oxygen species and the concomitant attenuation of tissue damage during the early phase of acute lung injury.

Original languageEnglish (US)
Pages (from-to)20-28
Number of pages9
JournalJournal of Critical Care
Volume14
Issue number1
DOIs
StatePublished - Mar 1999
Externally publishedYes

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Liquid Ventilation
Lung
Acute Lung Injury
Artificial Respiration
Oxygen
Proteins
Mortality
Alveolar Macrophages
Bronchoalveolar Lavage
Oleic Acid
Reactive Oxygen Species
Gases
Lipids
Control Groups
Wounds and Injuries

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Steinhorn, D. M., Papo, M. C., Rotta, A. T., Aljada, A., Fuhrman, B. P., & Dandona, P. (1999). Liquid ventilation attenuates pulmonary oxidative damage. Journal of Critical Care, 14(1), 20-28. https://doi.org/10.1016/S0883-9441(99)90004-7

Liquid ventilation attenuates pulmonary oxidative damage. / Steinhorn, David M.; Papo, Michèle C.; Rotta, Alexandre T.; Aljada, Ahmed; Fuhrman, Bradley P.; Dandona, Paresh.

In: Journal of Critical Care, Vol. 14, No. 1, 03.1999, p. 20-28.

Research output: Contribution to journalArticle

Steinhorn, DM, Papo, MC, Rotta, AT, Aljada, A, Fuhrman, BP & Dandona, P 1999, 'Liquid ventilation attenuates pulmonary oxidative damage', Journal of Critical Care, vol. 14, no. 1, pp. 20-28. https://doi.org/10.1016/S0883-9441(99)90004-7
Steinhorn DM, Papo MC, Rotta AT, Aljada A, Fuhrman BP, Dandona P. Liquid ventilation attenuates pulmonary oxidative damage. Journal of Critical Care. 1999 Mar;14(1):20-28. https://doi.org/10.1016/S0883-9441(99)90004-7
Steinhorn, David M. ; Papo, Michèle C. ; Rotta, Alexandre T. ; Aljada, Ahmed ; Fuhrman, Bradley P. ; Dandona, Paresh. / Liquid ventilation attenuates pulmonary oxidative damage. In: Journal of Critical Care. 1999 ; Vol. 14, No. 1. pp. 20-28.
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