Abstract
We developed a solid-phase extraction LC-MS-MS method for the analysis of the four major metabolites of PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) in human urine after a meal of well-done chicken. Ten volunteers each ate either 150 or 200 g of well-done chicken breast containing 9-21 μg of PhIP. Among the individual volunteers there is 8-fold variation in the total amount of metabolites and 20-fold variation in the relative amounts of individual metabolites, showing individual differences in carcinogen metabolism. PhIP metabolites were also detected in urine from a subject consuming chicken in a restaurant meal, demonstrating the method's sensitivity after real-life exposures.
Original language | English (US) |
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Pages (from-to) | 95-103 |
Number of pages | 9 |
Journal | Journal of Chromatography A |
Volume | 914 |
Issue number | 1-2 |
DOIs | |
State | Published - Apr 20 2001 |
Externally published | Yes |
Keywords
- Amines
- Aminomethylphenylimidazo[4,5-b]pyridine
- Glucuronides
- Heterocyclic aromatic
- Pyridines
ASJC Scopus subject areas
- Analytical Chemistry