Liquid chromatography-tandem mass spectrometry method of urine analysis for determining human variation in carcinogen metabolism

M. G. Knize; K. S. Kulp; M. A. Malfatti; C. P. Salmon; J. S. Felton

doi:10.1016/S0021-9673(01)00522-2

Liquid chromatography-tandem mass spectrometry method of urine analysis for determining human variation in carcinogen metabolism

M. G. Knize, K. S. Kulp, M. A. Malfatti, C. P. Salmon, J. S. Felton

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

We developed a solid-phase extraction LC-MS-MS method for the analysis of the four major metabolites of PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) in human urine after a meal of well-done chicken. Ten volunteers each ate either 150 or 200 g of well-done chicken breast containing 9-21 μg of PhIP. Among the individual volunteers there is 8-fold variation in the total amount of metabolites and 20-fold variation in the relative amounts of individual metabolites, showing individual differences in carcinogen metabolism. PhIP metabolites were also detected in urine from a subject consuming chicken in a restaurant meal, demonstrating the method's sensitivity after real-life exposures.

Original language	English (US)
Pages (from-to)	95-103
Number of pages	9
Journal	Journal of Chromatography A
Volume	914
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(01)00522-2
State	Published - Apr 20 2001
Externally published	Yes

Keywords

Amines
Aminomethylphenylimidazo[4,5-b]pyridine
Glucuronides
Heterocyclic aromatic
Pyridines

ASJC Scopus subject areas

Analytical Chemistry

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10.1016/S0021-9673(01)00522-2

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title = "Liquid chromatography-tandem mass spectrometry method of urine analysis for determining human variation in carcinogen metabolism",

abstract = "We developed a solid-phase extraction LC-MS-MS method for the analysis of the four major metabolites of PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) in human urine after a meal of well-done chicken. Ten volunteers each ate either 150 or 200 g of well-done chicken breast containing 9-21 μg of PhIP. Among the individual volunteers there is 8-fold variation in the total amount of metabolites and 20-fold variation in the relative amounts of individual metabolites, showing individual differences in carcinogen metabolism. PhIP metabolites were also detected in urine from a subject consuming chicken in a restaurant meal, demonstrating the method's sensitivity after real-life exposures.",

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author = "Knize, {M. G.} and Kulp, {K. S.} and Malfatti, {M. A.} and Salmon, {C. P.} and Felton, {J. S.}",

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AU - Knize, M. G.

AU - Kulp, K. S.

AU - Malfatti, M. A.

AU - Salmon, C. P.

AU - Felton, J. S.

PY - 2001/4/20

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N2 - We developed a solid-phase extraction LC-MS-MS method for the analysis of the four major metabolites of PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) in human urine after a meal of well-done chicken. Ten volunteers each ate either 150 or 200 g of well-done chicken breast containing 9-21 μg of PhIP. Among the individual volunteers there is 8-fold variation in the total amount of metabolites and 20-fold variation in the relative amounts of individual metabolites, showing individual differences in carcinogen metabolism. PhIP metabolites were also detected in urine from a subject consuming chicken in a restaurant meal, demonstrating the method's sensitivity after real-life exposures.

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KW - Amines

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KW - Glucuronides

KW - Heterocyclic aromatic

KW - Pyridines

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