Abstract
We developed a solid-phase extraction LC-MS-MS method for the analysis of the four major metabolites of PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) in human urine after a meal of well-done chicken. Ten volunteers each ate either 150 or 200 g of well-done chicken breast containing 9-21 μg of PhIP. Among the individual volunteers there is 8-fold variation in the total amount of metabolites and 20-fold variation in the relative amounts of individual metabolites, showing individual differences in carcinogen metabolism. PhIP metabolites were also detected in urine from a subject consuming chicken in a restaurant meal, demonstrating the method's sensitivity after real-life exposures.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-103 |
| Number of pages | 9 |
| Journal | Journal of Chromatography A |
| Volume | 914 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Apr 20 2001 |
| Externally published | Yes |
Keywords
- Amines
- Aminomethylphenylimidazo[4,5-b]pyridine
- Glucuronides
- Heterocyclic aromatic
- Pyridines
ASJC Scopus subject areas
- Analytical Chemistry
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Cite this
Knize, M. G., Kulp, K. S., Malfatti, M. A., Salmon, C. P., & Felton, J. S. (2001). Liquid chromatography-tandem mass spectrometry method of urine analysis for determining human variation in carcinogen metabolism. Journal of Chromatography A, 914(1-2), 95-103. https://doi.org/10.1016/S0021-9673(01)00522-2