Liquid chromatography-tandem mass spectrometry method of urine analysis for determining human variation in carcinogen metabolism

M. G. Knize, K. S. Kulp, M. A. Malfatti, C. P. Salmon, J. S. Felton

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We developed a solid-phase extraction LC-MS-MS method for the analysis of the four major metabolites of PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) in human urine after a meal of well-done chicken. Ten volunteers each ate either 150 or 200 g of well-done chicken breast containing 9-21 μg of PhIP. Among the individual volunteers there is 8-fold variation in the total amount of metabolites and 20-fold variation in the relative amounts of individual metabolites, showing individual differences in carcinogen metabolism. PhIP metabolites were also detected in urine from a subject consuming chicken in a restaurant meal, demonstrating the method's sensitivity after real-life exposures.

Original languageEnglish (US)
Pages (from-to)95-103
Number of pages9
JournalJournal of Chromatography A
Volume914
Issue number1-2
DOIs
StatePublished - Apr 20 2001
Externally publishedYes

Fingerprint

Liquid chromatography
Metabolites
Tandem Mass Spectrometry
Metabolism
Liquid Chromatography
Carcinogens
Mass spectrometry
Urine
Chickens
Meals
Volunteers
Restaurants
Solid Phase Extraction
Individuality
Breast
2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine

Keywords

  • Amines
  • Aminomethylphenylimidazo[4,5-b]pyridine
  • Glucuronides
  • Heterocyclic aromatic
  • Pyridines

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Liquid chromatography-tandem mass spectrometry method of urine analysis for determining human variation in carcinogen metabolism. / Knize, M. G.; Kulp, K. S.; Malfatti, M. A.; Salmon, C. P.; Felton, J. S.

In: Journal of Chromatography A, Vol. 914, No. 1-2, 20.04.2001, p. 95-103.

Research output: Contribution to journalArticle

Knize, M. G. ; Kulp, K. S. ; Malfatti, M. A. ; Salmon, C. P. ; Felton, J. S. / Liquid chromatography-tandem mass spectrometry method of urine analysis for determining human variation in carcinogen metabolism. In: Journal of Chromatography A. 2001 ; Vol. 914, No. 1-2. pp. 95-103.
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