Abstract
We developed a solid-phase extraction LC-MS-MS method for the analysis of the four major metabolites of PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) in human urine after a meal of well-done chicken. Ten volunteers each ate either 150 or 200 g of well-done chicken breast containing 9-21 μg of PhIP. Among the individual volunteers there is 8-fold variation in the total amount of metabolites and 20-fold variation in the relative amounts of individual metabolites, showing individual differences in carcinogen metabolism. PhIP metabolites were also detected in urine from a subject consuming chicken in a restaurant meal, demonstrating the method's sensitivity after real-life exposures.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-103 |
| Number of pages | 9 |
| Journal | Journal of Chromatography A |
| Volume | 914 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Apr 20 2001 |
| Externally published | Yes |
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Keywords
- Amines
- Aminomethylphenylimidazo[4,5-b]pyridine
- Glucuronides
- Heterocyclic aromatic
- Pyridines
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Liquid chromatography-tandem mass spectrometry method of urine analysis for determining human variation in carcinogen metabolism. / Knize, M. G.; Kulp, K. S.; Malfatti, M. A.; Salmon, C. P.; Felton, J. S.
In: Journal of Chromatography A, Vol. 914, No. 1-2, 20.04.2001, p. 95-103.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Liquid chromatography-tandem mass spectrometry method of urine analysis for determining human variation in carcinogen metabolism
AU - Knize, M. G.
AU - Kulp, K. S.
AU - Malfatti, M. A.
AU - Salmon, C. P.
AU - Felton, J. S.
PY - 2001/4/20
Y1 - 2001/4/20
N2 - We developed a solid-phase extraction LC-MS-MS method for the analysis of the four major metabolites of PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) in human urine after a meal of well-done chicken. Ten volunteers each ate either 150 or 200 g of well-done chicken breast containing 9-21 μg of PhIP. Among the individual volunteers there is 8-fold variation in the total amount of metabolites and 20-fold variation in the relative amounts of individual metabolites, showing individual differences in carcinogen metabolism. PhIP metabolites were also detected in urine from a subject consuming chicken in a restaurant meal, demonstrating the method's sensitivity after real-life exposures.
AB - We developed a solid-phase extraction LC-MS-MS method for the analysis of the four major metabolites of PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) in human urine after a meal of well-done chicken. Ten volunteers each ate either 150 or 200 g of well-done chicken breast containing 9-21 μg of PhIP. Among the individual volunteers there is 8-fold variation in the total amount of metabolites and 20-fold variation in the relative amounts of individual metabolites, showing individual differences in carcinogen metabolism. PhIP metabolites were also detected in urine from a subject consuming chicken in a restaurant meal, demonstrating the method's sensitivity after real-life exposures.
KW - Amines
KW - Aminomethylphenylimidazo[4,5-b]pyridine
KW - Glucuronides
KW - Heterocyclic aromatic
KW - Pyridines
UR - http://www.scopus.com/inward/record.url?scp=0035918026&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035918026&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)00522-2
DO - 10.1016/S0021-9673(01)00522-2
M3 - Article
C2 - 11358237
AN - SCOPUS:0035918026
VL - 914
SP - 95
EP - 103
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 1-2
ER -