Lipoxygenase activity in the gills of the freshwater mussel, Ligumia subrostrata

Arthur F. Hagar, Daniel H. Hwang, Thomas H. Dietz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Isolated gill tissue from freshwater mussels acclimated to pondwater, metabolize exogenous arachidonic acid via the lipoxygenase pathways. 5-Hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE) was identified as the principal metabolite using high-performance liquid chromatography (HPLC), radioimmunoassay (RIA), and gas chromatography mass spectrometry. Using HPLC and RIA, we also found 12-HETE to be produced at about 50% of the level of 5-HETE. Using HPLC, other lipoxygenase metabolites appeared to be synthesized by isolated gills but cyclooxygenase products were notably absent. However, both cyclooxygenase- and lipoxygenase-derived products of arachidonic acid metabolism are demonstrable in crude homogenates of mussel gills.

Original languageEnglish (US)
Pages (from-to)162-169
Number of pages8
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1005
Issue number2
DOIs
StatePublished - Sep 25 1989
Externally publishedYes

Fingerprint

Lipoxygenase
Bivalvia
High performance liquid chromatography
Fresh Water
High Pressure Liquid Chromatography
Prostaglandin-Endoperoxide Synthases
Metabolites
Arachidonic Acid
Radioimmunoassay
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Arachidonate Lipoxygenases
Metabolism
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Tissue
5-hydroxy-6,8,11,14-eicosatetraenoic acid

Keywords

  • (L. subrostrata)
  • 5-Hydroxyeicosatetraenoic acid
  • Arachidonic acid metabolism
  • Lipoxygenase
  • Mollusc
  • Pelecypoda

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

Cite this

Lipoxygenase activity in the gills of the freshwater mussel, Ligumia subrostrata. / Hagar, Arthur F.; Hwang, Daniel H.; Dietz, Thomas H.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 1005, No. 2, 25.09.1989, p. 162-169.

Research output: Contribution to journalArticle

Hagar, Arthur F. ; Hwang, Daniel H. ; Dietz, Thomas H. / Lipoxygenase activity in the gills of the freshwater mussel, Ligumia subrostrata. In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1989 ; Vol. 1005, No. 2. pp. 162-169.
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