Lipoxygenase activity in the gills of the freshwater mussel, Ligumia subrostrata

Arthur F. Hagar, Daniel H. Hwang, Thomas H. Dietz

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Isolated gill tissue from freshwater mussels acclimated to pondwater, metabolize exogenous arachidonic acid via the lipoxygenase pathways. 5-Hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE) was identified as the principal metabolite using high-performance liquid chromatography (HPLC), radioimmunoassay (RIA), and gas chromatography mass spectrometry. Using HPLC and RIA, we also found 12-HETE to be produced at about 50% of the level of 5-HETE. Using HPLC, other lipoxygenase metabolites appeared to be synthesized by isolated gills but cyclooxygenase products were notably absent. However, both cyclooxygenase- and lipoxygenase-derived products of arachidonic acid metabolism are demonstrable in crude homogenates of mussel gills.

Original languageEnglish (US)
Pages (from-to)162-169
Number of pages8
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Issue number2
StatePublished - Sep 25 1989
Externally publishedYes


  • (L. subrostrata)
  • 5-Hydroxyeicosatetraenoic acid
  • Arachidonic acid metabolism
  • Lipoxygenase
  • Mollusc
  • Pelecypoda

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology


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