Liposomal Gene Transfer of Keratinocyte Growth Factor Improves Wound Healing by Altering Growth Factor and Collagen Expression

Clifford Pereira, David N. Herndon, Roland Rocker, Marc G. Jeschke

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Background: Growth factors affect the complex cascade of wound healing; however, interaction between different growth factors during dermal and epidermal regeneration are still not entirely defined. In the present study, we thought to determine the interaction between keratinocyte growth factor (KGF) administered as liposomal cDNA with other dermal and epidermal growth factors and collagen synthesis in an acute wound. Materials and methods: Rats received an acute wound and were divided into two groups to receive weekly subcutaneous injections of liposomes plus the Lac-Z gene (0.22 μg, vehicle), or liposomes plus the KGF cDNA (2.2 μg) and Lac-Z gene (0.22 μg). Histological and immunohistochemical techniques were used to determine growth factor, collagen expression, and dermal and epidermal structure. Results: KGF cDNA increased insulin-like growth factor-I (IGF-I), insulin-like growth factor binding protein-3 (IGFBP-3), and fibroblast growth factor (FGF), decreased transforming growth factor-beta (TGF-β), while it had no effect on platelet-derived growth factor (PDGF) levels in the wound. KGF cDNA significantly increased collagen Type IV at both the wound edge as well as the wound bed, while it had no effect on collagen Type I and III. KGF cDNA increased re-epithelialization, improved dermal regeneration, and increased neovascularization. Conclusions: Exogenous administered KGF cDNA causes increases in IGF-I, IGF-BP3, FGF, and collagen IV and decreases TGF-β concentration. KGF gene transfer accelerates wound healing without causing an increase in collagen I or III.

Original languageEnglish (US)
Pages (from-to)222-228
Number of pages7
JournalJournal of Surgical Research
Volume139
Issue number2
DOIs
StatePublished - May 15 2007
Externally publishedYes

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Keywords

  • gene therapy
  • gene transfer
  • growth factors
  • KGF
  • skin
  • wound healing

ASJC Scopus subject areas

  • Surgery

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