Abstract
Background Previous reports have shown that lipoprotein lipase (LPL) activity significantly increases in the postprandial plasma associated with the increase of TG-rich lipoproteins. Therefore, we have reexamined those relationships using newly developed LPL assay with the different kinds of food intake. Methods Standard meal (n = 81), 50 g of fat (n = 54), 75 g of glucose (n = 25) and cookie (25 g fat and 75 g carbohydrate fat) (n = 28) were administered in generally healthy volunteers. Plasma LPL, HTGL and TC, TG, LDL-C, HDL-C, RLP-C and RLP-TG were determined at subsequent withdrawal after the food intake. Results Plasma TG, RLP-C and RLP-TG were significantly increased at 8 PM (2 h after dinner of standard meal) compared with 8 AM before breakfast within the same day. Also those parameters were significantly increased in 2–6 h after fat load. However, the concentrations and activities of LPL and HTGL did not significantly increase in association with an increase in the TG and remnant lipoproteins. Also LPL concentration did not significantly increase after glucose and “cookie test” within 4 h. Conclusion No significant increase of LPL activity was found at CM and VLDL overload after different kinds of food intake when reexamined by newly developed assay for LPL activity and concentration.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 204-210 |
| Number of pages | 7 |
| Journal | Clinica Chimica Acta |
| Volume | 464 |
| DOIs | |
| State | Published - Jan 1 2017 |
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Keywords
- Chylomicrons
- Hepatic triglyceride lipase
- Lipoprotein lipase
- Remnant lipoproteins
- RLP-C
- RLP-TG
- Triglycerides
- Very low density lipoproteins
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Biochemistry, medical
Cite this
Lipoprotein lipase does not increase significantly in the postprandial plasma. / Ishiyama, Nobuyoshi; Sakamaki, Kouji; Shimomura, Younosuke; Kotani, Kazuhiko; Tsuzaki, Kokoro; Sakane, Naoki; Miyashita, Kazuya; Fukamachi, Isao; Kobayashi, Junji; Stanhope, Kimber; Havel, Peter J; Kamachi, Keiko; Tanaka, Akira; Tokita, Yoshiharu; Machida, Tetsuo; Murakami, Masami; Nakajima, Katsuyuki.
In: Clinica Chimica Acta, Vol. 464, 01.01.2017, p. 204-210.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lipoprotein lipase does not increase significantly in the postprandial plasma
AU - Ishiyama, Nobuyoshi
AU - Sakamaki, Kouji
AU - Shimomura, Younosuke
AU - Kotani, Kazuhiko
AU - Tsuzaki, Kokoro
AU - Sakane, Naoki
AU - Miyashita, Kazuya
AU - Fukamachi, Isao
AU - Kobayashi, Junji
AU - Stanhope, Kimber
AU - Havel, Peter J
AU - Kamachi, Keiko
AU - Tanaka, Akira
AU - Tokita, Yoshiharu
AU - Machida, Tetsuo
AU - Murakami, Masami
AU - Nakajima, Katsuyuki
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Background Previous reports have shown that lipoprotein lipase (LPL) activity significantly increases in the postprandial plasma associated with the increase of TG-rich lipoproteins. Therefore, we have reexamined those relationships using newly developed LPL assay with the different kinds of food intake. Methods Standard meal (n = 81), 50 g of fat (n = 54), 75 g of glucose (n = 25) and cookie (25 g fat and 75 g carbohydrate fat) (n = 28) were administered in generally healthy volunteers. Plasma LPL, HTGL and TC, TG, LDL-C, HDL-C, RLP-C and RLP-TG were determined at subsequent withdrawal after the food intake. Results Plasma TG, RLP-C and RLP-TG were significantly increased at 8 PM (2 h after dinner of standard meal) compared with 8 AM before breakfast within the same day. Also those parameters were significantly increased in 2–6 h after fat load. However, the concentrations and activities of LPL and HTGL did not significantly increase in association with an increase in the TG and remnant lipoproteins. Also LPL concentration did not significantly increase after glucose and “cookie test” within 4 h. Conclusion No significant increase of LPL activity was found at CM and VLDL overload after different kinds of food intake when reexamined by newly developed assay for LPL activity and concentration.
AB - Background Previous reports have shown that lipoprotein lipase (LPL) activity significantly increases in the postprandial plasma associated with the increase of TG-rich lipoproteins. Therefore, we have reexamined those relationships using newly developed LPL assay with the different kinds of food intake. Methods Standard meal (n = 81), 50 g of fat (n = 54), 75 g of glucose (n = 25) and cookie (25 g fat and 75 g carbohydrate fat) (n = 28) were administered in generally healthy volunteers. Plasma LPL, HTGL and TC, TG, LDL-C, HDL-C, RLP-C and RLP-TG were determined at subsequent withdrawal after the food intake. Results Plasma TG, RLP-C and RLP-TG were significantly increased at 8 PM (2 h after dinner of standard meal) compared with 8 AM before breakfast within the same day. Also those parameters were significantly increased in 2–6 h after fat load. However, the concentrations and activities of LPL and HTGL did not significantly increase in association with an increase in the TG and remnant lipoproteins. Also LPL concentration did not significantly increase after glucose and “cookie test” within 4 h. Conclusion No significant increase of LPL activity was found at CM and VLDL overload after different kinds of food intake when reexamined by newly developed assay for LPL activity and concentration.
KW - Chylomicrons
KW - Hepatic triglyceride lipase
KW - Lipoprotein lipase
KW - Remnant lipoproteins
KW - RLP-C
KW - RLP-TG
KW - Triglycerides
KW - Very low density lipoproteins
UR - http://www.scopus.com/inward/record.url?scp=85002943475&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85002943475&partnerID=8YFLogxK
U2 - 10.1016/j.cca.2016.11.035
DO - 10.1016/j.cca.2016.11.035
M3 - Article
C2 - 27908779
AN - SCOPUS:85002943475
VL - 464
SP - 204
EP - 210
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
ER -