Lipoprotein lipase does not increase significantly in the postprandial plasma

Nobuyoshi Ishiyama, Kouji Sakamaki, Younosuke Shimomura, Kazuhiko Kotani, Kokoro Tsuzaki, Naoki Sakane, Kazuya Miyashita, Isao Fukamachi, Junji Kobayashi, Kimber Stanhope, Peter J Havel, Keiko Kamachi, Akira Tanaka, Yoshiharu Tokita, Tetsuo Machida, Masami Murakami, Katsuyuki Nakajima

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background Previous reports have shown that lipoprotein lipase (LPL) activity significantly increases in the postprandial plasma associated with the increase of TG-rich lipoproteins. Therefore, we have reexamined those relationships using newly developed LPL assay with the different kinds of food intake. Methods Standard meal (n = 81), 50 g of fat (n = 54), 75 g of glucose (n = 25) and cookie (25 g fat and 75 g carbohydrate fat) (n = 28) were administered in generally healthy volunteers. Plasma LPL, HTGL and TC, TG, LDL-C, HDL-C, RLP-C and RLP-TG were determined at subsequent withdrawal after the food intake. Results Plasma TG, RLP-C and RLP-TG were significantly increased at 8 PM (2 h after dinner of standard meal) compared with 8 AM before breakfast within the same day. Also those parameters were significantly increased in 2–6 h after fat load. However, the concentrations and activities of LPL and HTGL did not significantly increase in association with an increase in the TG and remnant lipoproteins. Also LPL concentration did not significantly increase after glucose and “cookie test” within 4 h. Conclusion No significant increase of LPL activity was found at CM and VLDL overload after different kinds of food intake when reexamined by newly developed assay for LPL activity and concentration.

Original languageEnglish (US)
Pages (from-to)204-210
Number of pages7
JournalClinica Chimica Acta
Volume464
DOIs
StatePublished - Jan 1 2017

Fingerprint

Lipoprotein Lipase
Plasmas
Fats
Meals
Eating
Lipoproteins
Assays
Glucose
Breakfast
Healthy Volunteers
Carbohydrates
Association reactions

Keywords

  • Chylomicrons
  • Hepatic triglyceride lipase
  • Lipoprotein lipase
  • Remnant lipoproteins
  • RLP-C
  • RLP-TG
  • Triglycerides
  • Very low density lipoproteins

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Ishiyama, N., Sakamaki, K., Shimomura, Y., Kotani, K., Tsuzaki, K., Sakane, N., ... Nakajima, K. (2017). Lipoprotein lipase does not increase significantly in the postprandial plasma. Clinica Chimica Acta, 464, 204-210. https://doi.org/10.1016/j.cca.2016.11.035

Lipoprotein lipase does not increase significantly in the postprandial plasma. / Ishiyama, Nobuyoshi; Sakamaki, Kouji; Shimomura, Younosuke; Kotani, Kazuhiko; Tsuzaki, Kokoro; Sakane, Naoki; Miyashita, Kazuya; Fukamachi, Isao; Kobayashi, Junji; Stanhope, Kimber; Havel, Peter J; Kamachi, Keiko; Tanaka, Akira; Tokita, Yoshiharu; Machida, Tetsuo; Murakami, Masami; Nakajima, Katsuyuki.

In: Clinica Chimica Acta, Vol. 464, 01.01.2017, p. 204-210.

Research output: Contribution to journalArticle

Ishiyama, N, Sakamaki, K, Shimomura, Y, Kotani, K, Tsuzaki, K, Sakane, N, Miyashita, K, Fukamachi, I, Kobayashi, J, Stanhope, K, Havel, PJ, Kamachi, K, Tanaka, A, Tokita, Y, Machida, T, Murakami, M & Nakajima, K 2017, 'Lipoprotein lipase does not increase significantly in the postprandial plasma', Clinica Chimica Acta, vol. 464, pp. 204-210. https://doi.org/10.1016/j.cca.2016.11.035
Ishiyama N, Sakamaki K, Shimomura Y, Kotani K, Tsuzaki K, Sakane N et al. Lipoprotein lipase does not increase significantly in the postprandial plasma. Clinica Chimica Acta. 2017 Jan 1;464:204-210. https://doi.org/10.1016/j.cca.2016.11.035
Ishiyama, Nobuyoshi ; Sakamaki, Kouji ; Shimomura, Younosuke ; Kotani, Kazuhiko ; Tsuzaki, Kokoro ; Sakane, Naoki ; Miyashita, Kazuya ; Fukamachi, Isao ; Kobayashi, Junji ; Stanhope, Kimber ; Havel, Peter J ; Kamachi, Keiko ; Tanaka, Akira ; Tokita, Yoshiharu ; Machida, Tetsuo ; Murakami, Masami ; Nakajima, Katsuyuki. / Lipoprotein lipase does not increase significantly in the postprandial plasma. In: Clinica Chimica Acta. 2017 ; Vol. 464. pp. 204-210.
@article{f57be953650a4d0e8ee9e0efb9ff4866,
title = "Lipoprotein lipase does not increase significantly in the postprandial plasma",
abstract = "Background Previous reports have shown that lipoprotein lipase (LPL) activity significantly increases in the postprandial plasma associated with the increase of TG-rich lipoproteins. Therefore, we have reexamined those relationships using newly developed LPL assay with the different kinds of food intake. Methods Standard meal (n = 81), 50 g of fat (n = 54), 75 g of glucose (n = 25) and cookie (25 g fat and 75 g carbohydrate fat) (n = 28) were administered in generally healthy volunteers. Plasma LPL, HTGL and TC, TG, LDL-C, HDL-C, RLP-C and RLP-TG were determined at subsequent withdrawal after the food intake. Results Plasma TG, RLP-C and RLP-TG were significantly increased at 8 PM (2 h after dinner of standard meal) compared with 8 AM before breakfast within the same day. Also those parameters were significantly increased in 2–6 h after fat load. However, the concentrations and activities of LPL and HTGL did not significantly increase in association with an increase in the TG and remnant lipoproteins. Also LPL concentration did not significantly increase after glucose and “cookie test” within 4 h. Conclusion No significant increase of LPL activity was found at CM and VLDL overload after different kinds of food intake when reexamined by newly developed assay for LPL activity and concentration.",
keywords = "Chylomicrons, Hepatic triglyceride lipase, Lipoprotein lipase, Remnant lipoproteins, RLP-C, RLP-TG, Triglycerides, Very low density lipoproteins",
author = "Nobuyoshi Ishiyama and Kouji Sakamaki and Younosuke Shimomura and Kazuhiko Kotani and Kokoro Tsuzaki and Naoki Sakane and Kazuya Miyashita and Isao Fukamachi and Junji Kobayashi and Kimber Stanhope and Havel, {Peter J} and Keiko Kamachi and Akira Tanaka and Yoshiharu Tokita and Tetsuo Machida and Masami Murakami and Katsuyuki Nakajima",
year = "2017",
month = "1",
day = "1",
doi = "10.1016/j.cca.2016.11.035",
language = "English (US)",
volume = "464",
pages = "204--210",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",

}

TY - JOUR

T1 - Lipoprotein lipase does not increase significantly in the postprandial plasma

AU - Ishiyama, Nobuyoshi

AU - Sakamaki, Kouji

AU - Shimomura, Younosuke

AU - Kotani, Kazuhiko

AU - Tsuzaki, Kokoro

AU - Sakane, Naoki

AU - Miyashita, Kazuya

AU - Fukamachi, Isao

AU - Kobayashi, Junji

AU - Stanhope, Kimber

AU - Havel, Peter J

AU - Kamachi, Keiko

AU - Tanaka, Akira

AU - Tokita, Yoshiharu

AU - Machida, Tetsuo

AU - Murakami, Masami

AU - Nakajima, Katsuyuki

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background Previous reports have shown that lipoprotein lipase (LPL) activity significantly increases in the postprandial plasma associated with the increase of TG-rich lipoproteins. Therefore, we have reexamined those relationships using newly developed LPL assay with the different kinds of food intake. Methods Standard meal (n = 81), 50 g of fat (n = 54), 75 g of glucose (n = 25) and cookie (25 g fat and 75 g carbohydrate fat) (n = 28) were administered in generally healthy volunteers. Plasma LPL, HTGL and TC, TG, LDL-C, HDL-C, RLP-C and RLP-TG were determined at subsequent withdrawal after the food intake. Results Plasma TG, RLP-C and RLP-TG were significantly increased at 8 PM (2 h after dinner of standard meal) compared with 8 AM before breakfast within the same day. Also those parameters were significantly increased in 2–6 h after fat load. However, the concentrations and activities of LPL and HTGL did not significantly increase in association with an increase in the TG and remnant lipoproteins. Also LPL concentration did not significantly increase after glucose and “cookie test” within 4 h. Conclusion No significant increase of LPL activity was found at CM and VLDL overload after different kinds of food intake when reexamined by newly developed assay for LPL activity and concentration.

AB - Background Previous reports have shown that lipoprotein lipase (LPL) activity significantly increases in the postprandial plasma associated with the increase of TG-rich lipoproteins. Therefore, we have reexamined those relationships using newly developed LPL assay with the different kinds of food intake. Methods Standard meal (n = 81), 50 g of fat (n = 54), 75 g of glucose (n = 25) and cookie (25 g fat and 75 g carbohydrate fat) (n = 28) were administered in generally healthy volunteers. Plasma LPL, HTGL and TC, TG, LDL-C, HDL-C, RLP-C and RLP-TG were determined at subsequent withdrawal after the food intake. Results Plasma TG, RLP-C and RLP-TG were significantly increased at 8 PM (2 h after dinner of standard meal) compared with 8 AM before breakfast within the same day. Also those parameters were significantly increased in 2–6 h after fat load. However, the concentrations and activities of LPL and HTGL did not significantly increase in association with an increase in the TG and remnant lipoproteins. Also LPL concentration did not significantly increase after glucose and “cookie test” within 4 h. Conclusion No significant increase of LPL activity was found at CM and VLDL overload after different kinds of food intake when reexamined by newly developed assay for LPL activity and concentration.

KW - Chylomicrons

KW - Hepatic triglyceride lipase

KW - Lipoprotein lipase

KW - Remnant lipoproteins

KW - RLP-C

KW - RLP-TG

KW - Triglycerides

KW - Very low density lipoproteins

UR - http://www.scopus.com/inward/record.url?scp=85002943475&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85002943475&partnerID=8YFLogxK

U2 - 10.1016/j.cca.2016.11.035

DO - 10.1016/j.cca.2016.11.035

M3 - Article

C2 - 27908779

AN - SCOPUS:85002943475

VL - 464

SP - 204

EP - 210

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

ER -