Abstract
A detailed evaluation of the antioxidant and pro-oxidant properties of lipoic acid (LA) and dihydrolipoic acid (DHLA) was performed. Both compounds are powerful scavengers of hypochlorous acid, able to protect α1-antiproteinase against inactivation by HOCl. LA was a powerful scavenger of hydroxyl radicals (OH-) and could inhibit both iron-dependent OH' generation and peroxidation of ox-brain phospholipid liposomes in the presence of FeCl3 -ascorbate, presumably by binding iron ions and rendering them redox-inactive. By contrast. DHLA accelerated iron-dependent OH- generation and lipid peroxidation, probably by reducing Fe3+ to Fe2+. LA inhibited this pro-oxidant action of DHLA. However, DHLA did not accelerate DNA degradation by a ferric bleomycin complex and slightly inhibited peroxidation of arachidonic acid by the myoglobin-H2O2 system. Under certain circumstances, DHLA accelerated the loss of activity of αantiproteinase exposed to ionizing radiation under a N2O/O2 atmosphere and also the loss of creatine kinase activity in human plasma exposed to gas-phase cigarette smoke. Neither LA nor DHLA reacted with superoxide radical (O2 -) or H2O2 at significant rates, but both were good scavengers of trichloromethylperoxyl radical (CCl3O2). We conclude that LA and DHLA have powerful antioxidant properties. However, DHLA can also exert pro-oxidant properties, both by its iron ion-reducing ability and probably by its ability to generate reactive sulphur-containing radicals that can damage certain proteins, such as α1-antiproteinase and creatine kinase.
Original language | English (US) |
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Pages (from-to) | 119-133 |
Number of pages | 15 |
Journal | Free Radical Research |
Volume | 20 |
Issue number | 2 |
DOIs | |
State | Published - 1994 |
Keywords
- Antioxidant
- Dihydrolipoic acid
- Hydroxyl radical
- Hypochlorous acid
- Lipid peroxidation
- Lipoic acid
- Superoxide
- Trichloromethylperoxyl radical
ASJC Scopus subject areas
- Biochemistry