Lipid raft distribution of CD4 depends on its palmitoylation and association with Lck, and evidence for CD4-induced lipid raft aggregation as an additional mechanism to enhance CD3 signaling

Ruben C Fragoso, Dejian Ren, Xiaoping Zhang, Michael Wei-Chih Su, Steven J. Burakoff, Yong Jiu Jin

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

By mutagenesis, we demonstrated that the palmitoylation of the membrane-proximal Cys396 and Cys399 of CD4, and the association of CD4 with Lck contribute to the enrichment of CD4 in lipid rafts. Ab cross-linking of CD4 induces an extensive membrane patching on the T cell surface, which is related to lipid raft aggregation. The lipid raft localization of CD4 is critical for CD4 to induce the aggregation of lipid rafts. The localization of CD4 in lipid rafts also correlates to the ability of CD4 to enhance receptor tyrosine phosphorylation. Thus, our data suggest that CD4-induced aggregation of lipid rafts may play an additional role in CD4 signaling besides its adhesion to MHC molecules and association with Lck.

Original languageEnglish (US)
Pages (from-to)913-921
Number of pages9
JournalJournal of Immunology
Volume170
Issue number2
StatePublished - Jan 15 2003
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Immunology

Cite this