Lipid peroxidation biomarkers for evaluating oxidative stress in equine neuroaxonal dystrophy

Carrie J. Finno, Krista E. Estell, Laramie Winfield, Scott Katzman, Matthew H. Bordbari, Erin N. Burns, Andrew D. Miller, Birgit Puschner, Cecilia K. Tran, Libin Xu

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Background: Equine neuroaxonal dystrophy/equine degenerative myeloencephalopathy (eNAD/EDM) is a neurodegenerative disorder affecting genetically predisposed foals maintained on an α-tocopherol (α-TOH) deficient diet. Currently no antemortem diagnostic test for eNAD/EDM is available. Hypothesis: Because α-TOH deficiency is associated with increased lipid peroxidation, it was hypothesized that F2-isoprostanes (F2IsoP), F4-neuroprostanes (F4NP) and oxysterols derived from free radical oxidation would be increased in the cerebrospinal fluid (CSF) and neural tissue of eNAD/EDM affected horses and could serve as potential biomarkers for disease. Animals: Isoprostane Study A: 14 Quarter horse foals (10 healthy foals and 4 eNAD/EDM affected foals) at 1 and 6 months of age. Isoprostane Study B: 17 eNAD/EDM affected and 10 unaffected horses ≥ 1-4 years of age. Oxysterol study: eNAD/EDM affected (n = 14, serum; n = 11, CSF; n = 10, spinal cord [SC]) and unaffected horses 1-4 years of age (n = 12, serum; n = 10, CSF; n = 7, SC). Procedures: Cerebrospinal fluid [F2IsoP] and [F4NP] were assessed using gas chromatography-negative ion chemical ionization mass spectrometry. Serum, CSF, and cervical SC [oxysterols] were quantified using high performance liquid chromatography mass spectrometry. Results were compared with respective α-TOH concentrations. Results: Spinal cord [7-ketocholesterol], [7-hydroxycholesterol], and [7-keto-27-hydrocholesterol] were higher in eNAD/EDM horses whereas [24-ketocholesterol] was lower. No significant difference was found in CSF [F2IsoP] and [F4NP], serum [oxysterols] and CSF [oxysterols] between eNAD/EDM affected and unaffected horses. No correlation was found between [F2IsoP], [F4NP], or [oxysterols] and respective [α-TOH]. Conclusions and Clinical Importance: In the SC, targeted markers of cholesterol oxidation were significantly increased in horses with eNAD/EDM.

Original languageEnglish (US)
Pages (from-to)1740-1747
Number of pages8
JournalJournal of Veterinary Internal Medicine
Volume32
Issue number5
DOIs
StatePublished - Sep 1 2018

Keywords

  • ataxia
  • equine
  • genetics
  • vitamin E

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint

Dive into the research topics of 'Lipid peroxidation biomarkers for evaluating oxidative stress in equine neuroaxonal dystrophy'. Together they form a unique fingerprint.

Cite this