Lipid Membrane Deformation Accompanied by Disk-to-Ring Shape Transition of Cholesterol-Rich Domains

Yong Sang Ryu, Daehan Yoo, Nathan J. Wittenberg, Luke R. Jordan, Sin Doo Lee, Atul N. Parikh, Sang Hyun Oh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

During vesicle budding or endocytosis, biomembranes undergo a series of lipid- and protein-mediated deformations involving cholesterol-enriched lipid rafts. If lipid rafts of high bending rigidities become confined to the incipient curved membrane topology such as a bud-neck interface, they can be expected to reform as ring-shaped rafts. Here, we report on the observation of a disk-to-ring shape morpho-chemical transition of a model membrane in the absence of geometric constraints. The raft shape transition is triggered by lateral compositional heterogeneity and is accompanied by membrane deformation in the vertical direction, which is detected by height-sensitive fluorescence interference contrast microscopy. Our results suggest that a flat membrane can become curved simply by dynamic changes in local chemical composition and shape transformation of cholesterol-rich domains.

Original languageEnglish (US)
Pages (from-to)8692-8695
Number of pages4
JournalJournal of the American Chemical Society
Volume137
Issue number27
DOIs
StatePublished - Jul 15 2015

Fingerprint

Cholesterol
Membrane Lipids
Lipids
Membranes
Interference Microscopy
Endocytosis
Fluorescence Microscopy
Rigidity
Microscopic examination
Neck
Fluorescence
Observation
Topology
Proteins
Chemical analysis

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Ryu, Y. S., Yoo, D., Wittenberg, N. J., Jordan, L. R., Lee, S. D., Parikh, A. N., & Oh, S. H. (2015). Lipid Membrane Deformation Accompanied by Disk-to-Ring Shape Transition of Cholesterol-Rich Domains. Journal of the American Chemical Society, 137(27), 8692-8695. https://doi.org/10.1021/jacs.5b04559

Lipid Membrane Deformation Accompanied by Disk-to-Ring Shape Transition of Cholesterol-Rich Domains. / Ryu, Yong Sang; Yoo, Daehan; Wittenberg, Nathan J.; Jordan, Luke R.; Lee, Sin Doo; Parikh, Atul N.; Oh, Sang Hyun.

In: Journal of the American Chemical Society, Vol. 137, No. 27, 15.07.2015, p. 8692-8695.

Research output: Contribution to journalArticle

Ryu, YS, Yoo, D, Wittenberg, NJ, Jordan, LR, Lee, SD, Parikh, AN & Oh, SH 2015, 'Lipid Membrane Deformation Accompanied by Disk-to-Ring Shape Transition of Cholesterol-Rich Domains', Journal of the American Chemical Society, vol. 137, no. 27, pp. 8692-8695. https://doi.org/10.1021/jacs.5b04559
Ryu, Yong Sang ; Yoo, Daehan ; Wittenberg, Nathan J. ; Jordan, Luke R. ; Lee, Sin Doo ; Parikh, Atul N. ; Oh, Sang Hyun. / Lipid Membrane Deformation Accompanied by Disk-to-Ring Shape Transition of Cholesterol-Rich Domains. In: Journal of the American Chemical Society. 2015 ; Vol. 137, No. 27. pp. 8692-8695.
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