Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega-3 fatty acids

Susanna L. Lundström, Jun Yang, John D. Brannan, Jesper Z. Haeggström, Bruce D. Hammock, Parameswaran Nair, Paul O'Byrne, Sven Erik Dahlén, Craig E. Wheelock

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Scope: In contrast to well-characterized PUFA levels in serum, little is known regarding their downstream metabolic products. However, many of these compounds are lipid mediators with prominent roles during pro- and antiinflammatory processes. Methods and results: In this double blind crossover study on asthmatics, shifts in serum levels of ω-3 and ω-6 PUFA-derived oxidized fatty acids (e.g. eicosanoids, oxylipins) were quantified following dietary fish oil supplementation. Serum was obtained from subjects following fasting at three occasions; (i) prior to supplementation, (ii) following a 3-week supplement intake of either placebo or fish oil, and (iii) following a 3-week washout period with a subsequent 3-week period of either fish oil or placebo supplement. A total of 87 oxylipins representing cyclooxygenase (COX), lipoxygenase (LOX), and cytochrome P450 (CYP) metabolic pathways were screened via LC-MS/MS. The primary alterations observed were in CYP- and 15-LOX-derived EPA- and CYP-derived DHA oxylipins. Conclusion: The results indicate that intake of an ω-3 rich diet alters not only the PUFA ratio, but also the ratio of downstream oxylipins. These data further support that dietary manipulation with ω-3 PUFAs affects not only PUFA levels, but importantly also the downstream metabolic profile.

Original languageEnglish (US)
Pages (from-to)1378-1389
Number of pages12
JournalMolecular Nutrition and Food Research
Volume57
Issue number8
DOIs
StatePublished - Aug 2013

Keywords

  • Asthma
  • Eicosanoid
  • Lipidomics
  • Omega-3
  • Oxylipin

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

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