Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega-3 fatty acids

Susanna L. Lundström, Jun Yang, John D. Brannan, Jesper Z. Haeggström, Bruce D. Hammock, Parameswaran Nair, Paul O'Byrne, Sven Erik Dahlén, Craig E. Wheelock

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Scope: In contrast to well-characterized PUFA levels in serum, little is known regarding their downstream metabolic products. However, many of these compounds are lipid mediators with prominent roles during pro- and antiinflammatory processes. Methods and results: In this double blind crossover study on asthmatics, shifts in serum levels of ω-3 and ω-6 PUFA-derived oxidized fatty acids (e.g. eicosanoids, oxylipins) were quantified following dietary fish oil supplementation. Serum was obtained from subjects following fasting at three occasions; (i) prior to supplementation, (ii) following a 3-week supplement intake of either placebo or fish oil, and (iii) following a 3-week washout period with a subsequent 3-week period of either fish oil or placebo supplement. A total of 87 oxylipins representing cyclooxygenase (COX), lipoxygenase (LOX), and cytochrome P450 (CYP) metabolic pathways were screened via LC-MS/MS. The primary alterations observed were in CYP- and 15-LOX-derived EPA- and CYP-derived DHA oxylipins. Conclusion: The results indicate that intake of an ω-3 rich diet alters not only the PUFA ratio, but also the ratio of downstream oxylipins. These data further support that dietary manipulation with ω-3 PUFAs affects not only PUFA levels, but importantly also the downstream metabolic profile.

Original languageEnglish (US)
Pages (from-to)1378-1389
Number of pages12
JournalMolecular Nutrition and Food Research
Volume57
Issue number8
DOIs
StatePublished - Aug 2013

Fingerprint

Oxylipins
oxylipins
Omega-3 Fatty Acids
Dietary Supplements
omega-3 fatty acids
blood lipids
dietary supplements
Fish Oils
fish oils
cytochrome P-450
Cytochrome P-450 Enzyme System
Lipids
lipoxygenase
Serum
placebos
Placebos
Arachidonate 15-Lipoxygenase
Unsaturated Dietary Fats
eicosanoids
Lipoxygenase

Keywords

  • Asthma
  • Eicosanoid
  • Lipidomics
  • Omega-3
  • Oxylipin

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

Cite this

Lundström, S. L., Yang, J., Brannan, J. D., Haeggström, J. Z., Hammock, B. D., Nair, P., ... Wheelock, C. E. (2013). Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega-3 fatty acids. Molecular Nutrition and Food Research, 57(8), 1378-1389. https://doi.org/10.1002/mnfr.201200827

Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega-3 fatty acids. / Lundström, Susanna L.; Yang, Jun; Brannan, John D.; Haeggström, Jesper Z.; Hammock, Bruce D.; Nair, Parameswaran; O'Byrne, Paul; Dahlén, Sven Erik; Wheelock, Craig E.

In: Molecular Nutrition and Food Research, Vol. 57, No. 8, 08.2013, p. 1378-1389.

Research output: Contribution to journalArticle

Lundström, SL, Yang, J, Brannan, JD, Haeggström, JZ, Hammock, BD, Nair, P, O'Byrne, P, Dahlén, SE & Wheelock, CE 2013, 'Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega-3 fatty acids', Molecular Nutrition and Food Research, vol. 57, no. 8, pp. 1378-1389. https://doi.org/10.1002/mnfr.201200827
Lundström, Susanna L. ; Yang, Jun ; Brannan, John D. ; Haeggström, Jesper Z. ; Hammock, Bruce D. ; Nair, Parameswaran ; O'Byrne, Paul ; Dahlén, Sven Erik ; Wheelock, Craig E. / Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega-3 fatty acids. In: Molecular Nutrition and Food Research. 2013 ; Vol. 57, No. 8. pp. 1378-1389.
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